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Nano probe having apoptosis targeting function as well as preparation method and application of nano probe

A nano-probe and targeting technology, which is applied in the field of tumor apoptosis nuclear magnetic resonance imaging nano-probe and its preparation and application, can solve the problems of complex operation and high price of polypeptides, and achieve uniform particle size and high biocompatibility , good biocompatibility

Inactive Publication Date: 2017-03-22
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The operation of the polypeptide used in this patent is complex and expensive

Method used

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  • Nano probe having apoptosis targeting function as well as preparation method and application of nano probe
  • Nano probe having apoptosis targeting function as well as preparation method and application of nano probe
  • Nano probe having apoptosis targeting function as well as preparation method and application of nano probe

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Embodiment 1

[0031] A nanoprobe with apoptosis targeting function for nuclear magnetic resonance imaging of tumor apoptosis, including Fe@Fe 3 o 4 Nanoparticles, coupled in Fe@Fe 3 o 4 MNPs-DSPE-PEG-DPA-Zn nanoprobe composed of liposome DSPE-PEG on the surface of nanoparticles and apoptosis targeting probe DPA-Zn. The diameter of the MNPs-DSPE-PEG-DPA-Zn nanometer probe is 12-13nm.

[0032] The preparation method of the above-mentioned nanoprobe with apoptosis targeting function for nuclear magnetic resonance imaging of tumor apoptosis comprises the following steps: take 2 mg DSPE-PEG-DBCO and 8 mg DSPE-PEG dissolved in 2 mL chloroform, add 5 mg Fe@Fe 3 o 4 Nanoparticles were shaken at room temperature overnight, chloroform was removed by rotary evaporation at room temperature, and then rotary steamed in a water bath at 80 ° C for 1 min, then 5 mL of water was added, and N 3 -DPA-Zn 2mg, dialyzed with 8000~14000Da dialysis bag for 48h, filtered with 0.22μm filter membrane to obtain 1m...

Embodiment 2

[0034] A nanoprobe with apoptosis targeting function for nuclear magnetic resonance imaging of tumor apoptosis, including Fe@Fe 3 o 4 Nanoparticles, coupled in Fe@Fe 3 o 4 MNPs-DSPE-PEG-DPA-Zn nanoprobe composed of liposome DSPE-PEG on the surface of nanoparticles, liposome DSPE-PEG-DBCO and apoptosis targeting probe DPA-Zn. The diameter of the MNPs-DSPE-PEG-DPA-Zn nanometer probe is 12-13nm.

[0035] The preparation method of the above-mentioned nanoprobe with apoptosis targeting function for nuclear magnetic resonance imaging of tumor apoptosis comprises the following steps: take 1mg DSPE-PEG-DBCO and 9mg DSPE-PEG dissolved in 2mL chloroform, add 5mg Fe@Fe 3 o 4 Nanoparticles, shake the bed overnight at room temperature, remove chloroform by rotary evaporation at room temperature, then add 5 mL of water, add N 3 -DPA-Zn 1mg, dialyzed with 8000~14000Da dialysis bag for 48h, filtered with 0.22μm filter membrane to obtain 1mg / mL nanoprobe MNPs-DSPE-PEG-DPA-Zn solution, sto...

Embodiment 3

[0037] A nanoprobe with apoptosis targeting function for nuclear magnetic resonance imaging of tumor apoptosis, including Fe@Fe 3 o 4 Nanoparticles, coupled in Fe@Fe 3 o 4 MNPs-DSPE-PEG-DPA-Zn nanoprobe composed of liposome DSPE-PEG on the surface of nanoparticles, liposome DSPE-PEG-DBCO and apoptosis targeting probe DPA-Zn. The diameter of the MNPs-DSPE-PEG-DPA-Zn nanometer probe is 12-13nm.

[0038] The above method for preparing nanoprobes with apoptosis targeting function for nuclear magnetic resonance imaging of tumor apoptosis comprises the following steps: dissolve 3 mg DSPE-PEG-DBCO and 7 mg DSPE-PEG in 2 mL chloroform, add 5 mg Fe@Fe 3 o 4 Nanoparticles, shake the bed overnight at room temperature, remove chloroform by rotary evaporation at room temperature, then add 5 mL of water, add N 3 -DPA-Zn3mg, dialyzed with 8000~14000Da dialysis bag for 48h, filtered with 0.22μm filter membrane to obtain 1mg / mL nanoprobe MNPs-DSPE-PEG-DPA-Zn solution, stored in refrigerat...

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Abstract

The invention relates to a nano probe having an apoptosis targeting function as well as a preparation method and an application of the nano probe. The MNPs DSPE PEG DPA Zn nano probe is composed of Fe@Fe3O4 nanoparticles, liposomes DSPE PEG coupled on the surface layers of the Fe@Fe3O4 nanoparticles and DPA Zn modified liposomes, wherein the prepared nano probe MNPs DSPE PEG DPA Zn is applicable to tumor apoptosis MRI (magnetic resonance imaging). In comparison with the prior art, the nano probe provided by the invention has the advantages that annexin or related polypeptides are replaced by cheap DPA-Zn, the DPA-Zn is grated to the surfaces of the nanoparticles by virtue of a simple and convenient non-catalysis Click reaction, tum apoptosis is detected by virtue of a non-invasive MRI means, and the like.

Description

technical field [0001] The invention relates to a multifunctional nanoprobe, in particular to a nanoprobe with apoptosis targeting function for nuclear magnetic resonance imaging of tumor apoptosis and its preparation and application. Background technique [0002] Apoptosis is the autonomous and orderly death of cells controlled by a series of genes, or programmed cell death. Apoptosis has been one of the hottest areas of biological research since it was first described by Kerr et al. in 1970. The initiation and implementation of the apoptosis process involves a series of complex biochemical reactions and is regulated by a variety of molecules. Certain characteristics of apoptosis genes and proteins make them attractive targets for tumor therapy. There are two main apoptotic pathways: the exogenous pathway and the intrinsic pathway, also known as the death receptor pathway and the mitochondrial pathway, respectively. The cascade reaction of the caspase family (Caspases) is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/12A61K49/10A61K49/08
Inventor 周治国赵恒周萍王丹利潘立星杨仕平
Owner SHANGHAI NORMAL UNIVERSITY
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