3D-printing artificial skin and preparation method thereof
A 3D printing and 3D printer technology, applied in the field of medical devices, achieves the effects of low price, short preparation time and abundant raw materials
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Embodiment 1
[0027] A preparation method for 3D printing artificial leather, comprising the following steps:
[0028] S1: Prepare 0.5mol / L acetic acid solution: dilute acetic acid with deionized water to obtain 0.5mol / L acetic acid solution;
[0029] S2: Preparation of collagen and sodium citrate mixed gel: take 100mL of acetic acid solution prepared in S1, add 4.9g of collagen, and then add 0.1g of anticoagulant composed of sodium citrate and sodium citrate to obtain collagen / anticoagulant Coagulant mixed gel, the mass ratio of described sodium citrate and sodium citrate is 1:1;
[0030] S3: Add the collagen / sodium citrate mixed gel prepared in S2 into the trough of the 3D printer, set the program of the 3D printer according to the three-dimensional data of the damaged skin area obtained by CT scanning, and start the printer to obtain a three-dimensional The dermis layer with a porous structure is solidified after freeze-drying, and the porosity of the three-dimensional porous structure ...
Embodiment 2
[0034] S1: Prepare 0.5mol / L acetic acid solution: dilute acetic acid with deionized water to obtain 0.5mol / L acetic acid solution;
[0035] S2: Preparation of collagen and sodium citrate mixed gel: take 100mL of acetic acid solution prepared in S1, add 5.9g of collagen, and then add 0.1g of anticoagulant composed of sodium citrate, sodium citrate and EDTA to obtain collagen / anticoagulant mixed gel, the mass ratio of the sodium citrate, sodium citrate and EDTA is 1:1:1;
[0036] S3: Add the collagen / sodium citrate mixed gel prepared in S2 into the trough of the 3D printer, set the program of the 3D printer according to the three-dimensional data of the damaged skin area obtained by CT scanning, and start the printer to obtain a three-dimensional The dermis layer with a porous structure is solidified after freeze-drying, and the porosity of the three-dimensional porous structure is 85%, and the pore size is 250 μm;
[0037] S4: Coating one layer of silicone gel layer on the co...
Embodiment 3
[0040] S1: Prepare 0.5mol / L acetic acid solution: dilute acetic acid with deionized water to obtain 0.5mol / L acetic acid solution;
[0041] S2: Preparation of collagen and sodium citrate mixed gel: take 100mL of acetic acid solution prepared in S1, add 6.9g of collagen, and then add 0.1g of anticoagulant composed of sodium citrate, sodium citrate and sodium oxalate to obtain Collagen / anticoagulant mixed gel, the mass ratio of described sodium citrate, sodium citrate and sodium oxalate is 1:1:1;
[0042] S3: Add the collagen / sodium citrate mixed gel prepared in S2 into the trough of the 3D printer, set the program of the 3D printer according to the three-dimensional data of the damaged skin area obtained by CT scanning, and start the printer to obtain a three-dimensional The dermis layer with a porous structure is solidified after freeze-drying, and the porosity of the three-dimensional porous structure is 85%, and the pore size is 250 μm;
[0043] S4: Coating one layer of sil...
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