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A kind of high-efficiency photoautotrophic culture method of Chlorella

A technology for photoautotrophic culture and chlorella, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve problems such as energy consumption, consumption, and discomfort of algae, and achieve vitality and improve Growth rate, the effect of prolonging the continuous culture period

Active Publication Date: 2019-05-31
HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Autotrophic culture has the disadvantages of low culture density and long growth cycle; combined culture has the problem of energy consumption caused by the need to provide ready-made organic carbon sources as the energy source for its growth. body discomfort, resulting in the death of some of the algae and other disadvantages; heterotrophic culture also consumes the ready-made organic carbon source, and the nutrients accumulated in the heterotrophic Chlorella cells, such as protein, chlorophyll, vitamins, etc. Low accumulation of related substances in autotrophic Chlorella

Method used

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  • A kind of high-efficiency photoautotrophic culture method of Chlorella
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  • A kind of high-efficiency photoautotrophic culture method of Chlorella

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Effect test

Embodiment 1

[0016] First, add the nutrient salts in the following table 1 into the tap water. After the components are fully dissolved, they are sterilized by ultraviolet light and added to a 5-ton photobioreactor to inoculate Chlorella, and ensure that the algae cell density is 1 million / milliliters, start chlorella photoautotrophy; autotrophic culture conditions are: use natural light, daytime light intensity is 10-130Klx, photoperiod 12 / 12, air flow rate 1.0L / min, carbon dioxide gas flow rate 0.05L / min, the temperature of the algae liquid is 26-33°C, and the pH is maintained at 7.0-9.0; from the 6th day of cultivation, harvest once every other day, harvest 50% of the algae liquid each time, and then add fresh culture liquid to continue the cultivation , until the 20th day, the culture ended. The harvested chlorella was centrifuged, cleaned, and dried to obtain dry algae powder, and the dry base of the algae and the protein content in the algae were measured. During the entire cultur...

Embodiment 2

[0021] First, add the nutrient salts in the following table 2 components into the tap water. After the components are fully dissolved, they are sterilized by ultraviolet light and added to a 5-ton photobioreactor to inoculate chlorella, and ensure that the algae cell density is 1.2 million / milliliters, start chlorella photoautotrophy; autotrophic culture conditions are: use natural light, daytime light intensity is 10-130Klx, photoperiod 12 / 12, air flow rate 10.0L / min, carbon dioxide gas flow rate 0.5L / min, the temperature of the algae liquid is 26-33°C, and the pH is maintained at 7.0-9.0; from the 6th day of cultivation, harvest once every other day, harvest 50% of the algae liquid each time, and then add fresh culture liquid to continue the cultivation , until the 20th day, the culture ended. The harvested chlorella was centrifuged, cleaned, and dried to obtain dry algae powder, and the dry base of the algae and the protein content in the algae were measured. During the ...

Embodiment 3

[0026] First, add the nutrient salts of the following table 3 components into the tap water. After the components are fully dissolved, they are sterilized by ultraviolet light and added to a 5-ton photobioreactor to inoculate Chlorella, and ensure that the algae cell density is 1.4 million / milliliters, start chlorella photoautotrophy; autotrophic culture conditions are: use natural light, daytime light intensity is 10-130Klx, photoperiod 12 / 12, air volume 5.0L / min, carbon dioxide gas inflow 0.2L / min, the temperature of the algae liquid is 26-33°C, and the pH is maintained at 7.0-9.0; from the 6th day of cultivation, harvest once every other day, harvest 50% of the algae liquid each time, and then add fresh culture liquid to continue the cultivation , until the 20th day, the culture ended. The harvested chlorella was centrifuged, cleaned, and dried to obtain dry algae powder, and the dry base of the algae and the protein content in the algae were measured. During the entire ...

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Abstract

The invention provides a high-efficiency photoautotrophic culture method for chlorella, adding culture solution into a photobioreactor, inserting chlorella, and ensuring that the algae cell density is ≥ 1 million / ml, using natural light, and the light intensity during the day At 10-130Klx, photoperiod 12 / 12, air flow rate 1.0-10.0L / min, carbon dioxide gas flow rate 0.05-0.5L / min, algae liquid temperature 26-33℃, pH maintained at 7.0-9.0; The invention realizes high-efficiency large-scale production with long continuous culture time, high yield, and high utilization rate of nutrients. During the entire culture period, the dry base of algae harvested is 22.131-23.181 kg, the protein content of algae reaches more than 55%, and the nutrients The utilization rate reaches more than 90%.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, in particular to a high-efficiency photoautotrophic culture method for chlorella. Background technique [0002] Chlorella is a general unicellular green algae belonging to the phylum Chlorella. It is a spherical and elliptical single-celled algae with a diameter of 3 to 8 microns. It is one of the earliest life on earth and appeared in more than 2 billion years. Formerly, it is a highly efficient photosynthetic plant that grows and reproduces by photosynthetic autotrophy. It is distributed all over the world, mostly living in small shallow water, and there are also seafood species. There are few individuals under natural conditions, and they are reproduced in large numbers in artificial culture. The protein, fat and carbohydrate content in the cells are very high, and there are many kinds of vitamins, which can be eaten and used as bait. [0003] Photosynthesis can be simply summarized a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12M21/02
Inventor 张俊杰云天颖黄循杰李汉仁倪王碧
Owner HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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