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Process for preparing selenium yeast protein peptide powder from selenium-rich yeast

A technology of selenium-enriched yeast and selenium yeast, which is applied in the direction of fermentation, skin care preparations, cosmetics, etc., can solve the problems of low ultrasonic crushing efficiency, limited application of selenoprotein, easy protein inactivation, etc., to enhance human immunity, The effect of unique production process and high technical content

Inactive Publication Date: 2017-03-22
安徽省华信生物药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing methods for preparing selenoproteins in selenium-enriched yeast usually use ultrasound, acid extraction or repeated freezing and thawing to break the yeast cells and then extract the selenoproteins. Inactivation, less purified protein in the later stage, which greatly limits the application of selenoproteins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A process for preparing selenium yeast protein peptide powder using selenium-enriched yeast, the specific method steps are as follows:

[0032] (1) Using selenium-enriched yeast products as raw materials, first dissolving selenium-enriched yeast in water to make a suspension with a concentration of 10% (w / w);

[0033] (2) Autolyze the suspension containing selenium-enriched yeast at a temperature of 53°C and a natural pH value for 2 hours, and add a plasmolytic agent during the autolysis stage, wherein the amount of the plasmolytic agent is 1% of the weight of the suspension ;

[0034] Autolysis is the use of endogenous enzymes (protease, nuclease, carbohydrate hydrolase, etc.) in the yeast to partially enzymolyze macromolecular substances such as the cell wall of the yeast to reduce production costs and produce flavor components.

[0035] The above-mentioned plasmolytic agent is one of sodium chloride or phosphate buffer or a mixture of both.

[0036] (3) Adjust the ...

Embodiment 2

[0044] A process for preparing selenium yeast protein peptide powder using selenium-enriched yeast, the specific method steps are as follows:

[0045](1) Using selenium-enriched yeast products as raw materials, first dissolving selenium-enriched yeast in water to make a suspension with a concentration of 15% (w / w);

[0046] (2) Autolyze the suspension containing selenium-enriched yeast at a temperature of 55°C and a natural pH value for 2 hours, and add a plasmolytic agent during the autolysis stage, wherein the amount of the plasmolytic agent is 2% of the weight of the suspension ;

[0047] Autolysis is the use of endogenous enzymes (protease, nuclease, carbohydrate hydrolase, etc.) in the yeast to partially enzymolyze macromolecular substances such as the cell wall of the yeast to reduce production costs and produce flavor components.

[0048] The above-mentioned plasmolytic agent is one of sodium chloride or phosphate buffer or a mixture of both.

[0049] (3) Adjust the p...

Embodiment 3

[0057] A process for preparing selenium yeast protein peptide powder using selenium-enriched yeast, the specific method steps are as follows:

[0058] (1) Using selenium-enriched yeast products as raw materials, first dissolving selenium-enriched yeast in water to make a suspension with a concentration of 12% (w / w);

[0059] (2) Autolyze the suspension containing selenium-enriched yeast at a temperature of 54°C and a natural pH value for 2 hours, and add a plasmolytic agent during the autolysis stage, wherein the amount of the plasmolytic agent is 1.5% of the weight of the suspension ;

[0060] Autolysis is the use of endogenous enzymes (protease, nuclease, carbohydrate hydrolase, etc.) in the yeast to partially enzymolyze macromolecular substances such as the cell wall of the yeast to reduce production costs and produce flavor components.

[0061] The above-mentioned plasmolytic agent is one of sodium chloride or phosphate buffer or a mixture of both.

[0062] (3) Adjust th...

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Abstract

The invention provides a process for preparing selenium yeast protein peptide powder from selenium-rich yeast. The process comprises the following steps: (1) with a selenium-rich yeast product as a raw material, dissolving selenium-rich yeast in water to prepare a suspension with a concentration of 10 to 15%; (2) subjecting the suspension containing the selenium-rich yeast to autolysis at a temperature of 53 to 55 DEG C under the condition of a natural pH value and adding plasmolyticum in the phase of autolysis; (3) adjusting the pH value of the suspension having undergone autolysis to 6 to 7 and adding a composite bio-enzyme preparation so as to prepare enzymatic hydrolysate for subsequent usage; (4) subjecting the enzymatic hydrolysate to hydrolysis at a temperature of 55 + / - 2 DEG C for 20 to 24 h, heating hydrolysate to 85 to 90 DEG C after hydrolysis, maintaining the temperature for 2 h, inactivating enzyme in the solution so as to guarantee stable product quality and high yield, then carrying out ultrafiltration and collecting a filtrate so as to obtain a polypeptide filtrate; (5) subjecting the polypeptide filtrate obtained after ultrafiltration to nanofiltration and desalination so as to obtain a desalinized peptide solution; and (6) subjecting the desalinized peptide solution to concentration and drying so as to obtain the selenium yeast protein peptide powder.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a process for preparing selenium yeast protein peptide powder by utilizing selenium-enriched yeast. Background technique [0002] Protein is the material basis of life and an important substance in the life activities of the human body. Every tissue cell and all important physiological activities in the body have protein participation. Without protein, there is no life. [0003] A protein is a biomacromolecule composed of one or more polypeptide chains. Each polypeptide chain contains several to hundreds of amino acid residues. The peptide chains are arranged in a certain order to form proteins with various functions. [0004] Protein oligopeptide (composed of 3-9 amino acid molecules) is a protein hydrolyzate obtained after the protein is hydrolyzed by protease and other biological enzymes, and then processed by a special process. The hydrolyzate also contains a small amo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A61K8/64A61Q19/08
CPCC12P21/06A61K8/64A61Q19/08C07K1/34
Inventor 杨思林魏春厅倪志祥
Owner 安徽省华信生物药业股份有限公司
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