MS medium for tissue culture of rouge radish, clustering bud medium and tissue culture rapid propagation method of rouge radish

Abstract

The invention provides an MS culture medium used for carmine radish tissue culturing, a multiple shoot culture medium and a carmine radish tissue culture rapid breeding method. The MS culture medium comprises NH4NO3, KNO3, CaCl2.2H2O, MgSO4.7H2O, KH2PO4, KI, H2BO3, MnSO4.4H2O, ZnSO4.7H2O, Na2MoO4.5H2O, CuSO4.5H2O, CoCl2.6H2O, FeSO4.7H2O, Na2-EDTA.2H2O, inositol, niacin, glycine, pyridoxine hydrochloride and thiamine hydrochloride. On the basis of the MS culture medium, the multiple shoot culture medium further comprises 6-BA, NAA, 2,4-D, TDZ, KT, saccharose and agar. The method comprises the steps of sterile explant obtaining, primary culture, subculture, strong seedling culture, rooting culture and acclimatization and transplant.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to an MS medium for tissue culture of rouge radish, a culture medium for clustered buds and a rapid propagation method for tissue culture of rouge radish. Background technique [0002] Rouge radish is a biennial vegetable crop of the genus Raphanus in the family Brassicaceae, also known as Fuling red radish. It is a specialty product in Fuling, Chongqing. It has a dense texture and a red carpel. It is a natural radish red pigment extracted from its fleshy roots The content, quality and solubility are all better than other carrots, and the color value is much higher than the national standard level. It has broad application prospects in the fields of food, wine, beverages and cosmetics. [0003] At present, carmine radish mainly reproduces sexually, and the endogenous bacteria infection is serious, the yield is low, and the variety is degraded, resulting in i...

AI Technical Summary

Problems solved by technology

The present invention aims at the technical problems that radish tissue culture is difficult to form callus, not easy to sprout, and the components and the concentration of each component in the existing MS medium are difficult to induce radish to produce callus, and the induced budding rate is low, and provides An MS medium for tissue culture of rouge radish, specifically, the type selection of mineral nutrients in the MS medium of the present invention is more suitable for the growth of rouge radish, and can better ensure the growth requirements of radish tissue, and the MS medium of the present invention passes nitrogen , phosphorus, potassium, cobalt, and vitamins can meet the nutritional and physiological requirements of rouge radish for cell dedifferentiation, provide energy for the generation of rouge radish callus, and can promote the acceleration of rouge radish callus growth, the use of the MS medium of the present invention has a callus induction effect on various types of rouge radish, and solves the technical problem that the existing MS medium cannot make rouge radish produce callus