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Sample preparation and detection of oil fatty acids and accompanied components

A sample preparation and fatty acid technology, applied in the direction of measuring devices, material separation, analysis of materials, etc., can solve the problems of difficult to obtain samples to be tested, cumbersome processing methods, etc., and achieve the effect of good repeatability, simple operation, and short time

Inactive Publication Date: 2017-04-19
NANYANG INST OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sample processing method for the detection of accompanying components and fatty acids in vegetable oils by gas chromatography is relatively cumbersome, and it is difficult to obtain a rapid processing method to simultaneously separate the fatty acids of the oil and the main accompanying components to be tested

Method used

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  • Sample preparation and detection of oil fatty acids and accompanied components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The preparation and detection of a kind of fatty acid of oil and main accompanying component, specifically operate according to the following steps:

[0014] 1. Take 0.5 grams of gardenia oil into a 10 mL stoppered test tube.

[0015] 2. Add 3 mL of 0.5 mol / L KOH-methanol solution, shake gently, place in a 65°C water bath for 45 minutes for saponification, and take it out.

[0016] 3. Add 3 mL of petroleum ether or n-hexane for extraction, stand still for 1 hour and draw the supernatant into a sample bottle for determination of accompanying components (according to claim 3). As a result, the vitamin E content was detected to be 0.05%, the sterol content was 0.2%, and the squalene content was 4%.

[0017] 4. After removing excess petroleum ether, add 0.4 mL BF to the lower layer 3 - Diethyl ether solution, placed in a water bath at 65°C, shaken gently, methylated for 10 min, rinsed with tap water to cool immediately after taking it out.

[0018] 5. Add 3 mL of petrole...

Embodiment 2

[0020] The preparation and detection of a kind of fatty acid of oil and main accompanying component, specifically operate according to the following steps:

[0021] 1. Take 0.5 g of seabuckthorn seed oil and put it into a 10 mL stoppered test tube.

[0022] 2. Add 3 mL of 0.5 mol / L KOH-methanol solution, shake gently, place in a 65°C water bath for 45 minutes for saponification, and take it out.

[0023] 3. Add 3 mL of petroleum ether or n-hexane for extraction, stand still for 1 hour and draw the supernatant into a sample bottle for accompanying component determination (according to claim 3). As a result, 0.08% vitamin E and 0.5% sterol were detected.

[0024] 4. After removing excess petroleum ether, add BF to the lower layer 3 - Diethyl ether solution 0.4 mL, placed in a water bath at 65°C, shaken gently, methylated for 10 min, rinsed with tap water to cool immediately after taking it out.

[0025] 5. Add 3 mL of petroleum ether or n-hexane for extraction, draw the supern...

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Abstract

The invention relates to sample preparation and detection of oil fatty acids and main accompanied components, and in particular, for the problems that when plant oil fatty acids and oil accompanied components of sterols, squalene, vitamin E and the like have different treatment methods during determination, gas chromatography detection can be performed after the fatty acids and the accompanied components need to be treated separately and the requiring time is longer, according to the properties of the fatty acids and the oil accompanied components of sterols, squalene and vitamin E, a sample treatment method which not only can extract saponified substances (fatty acids) but also can extract the accompanied components (non-saponified substances of sterols, squalene, vitamin E and the like) is determined, and the reliable method which can adopt gas phase chromatography for rapidly detecting the fatty acids and the accompanied components is provided for enterprises.

Description

technical field [0001] The invention relates to the field of determination and analysis of fatty acids and accompanying components in the oil industry, in particular to sample preparation and detection of fatty acids and accompanying components in oil and fat. Background technique [0002] Fatty acids in vegetable oils are a class of compounds composed of carbon, hydrogen, and oxygen, and are the main components of phospholipids, neutral fats, and glycolipids. The main components of fatty acids in oil are linolenic acid, linoleic acid, oleic acid, palmitic acid, stearic acid and so on. To evaluate the quality of vegetable oil, the amount of unsaturated fatty acid is often used as an index. Therefore, gas chromatography and gas chromatography-mass spectrometry detection methods are used to identify the type and content of fatty acid in oil, and the sample processing is relatively mature. A large number of studies have shown that the non-saponifiable components in vegetable o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8813
Inventor 薛刚宋伟翟润孙连贵王莹刘凤霞王伟锋杨明超王文伟
Owner NANYANG INST OF TECH
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