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Method for preparing double drug-carrying liposomes for treating Parkinson's disease

A technology of Parkinson's disease and double drug loading is applied in the field of preparation of drug-loaded liposomes, which can solve the problems of difficult absorption, poor drug effect, and no Parkinson's disease therapeutic preparations, etc. safety effect

Inactive Publication Date: 2017-04-26
温州市中医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its fat solubility is too strong, it is difficult to absorb when taken orally, and the drug effect is not good.
[0005] There is no Parkinson's disease treatment agent that can simultaneously stimulate endogenous bFGF production and supplement exogenous bFGF

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Weigh out 2mg of icariin, 30mg of soybean phospholipids, and 8mg of cholesterol, and dissolve them in 15ml of chloroform / methanol (2:1) mixed solvent. Place the solution in a 100ml ground-bottom flask and place it on a 30℃ constant temperature water bath. Dissolve uniformly and fully, take bFGF (2mg dissolved in PBS solution) solution and add dropwise to the above solution, ultrasonic probe ultrasonic probe for 3s, interval 10s, cycle 5 times, power 200W, to yellow emulsion, rotary vacuum evaporation to remove The organic solvent appeared gelatinous at the bottom of the round-bottom flask, and continued to evaporate under reduced pressure until the gel collapsed. Add an appropriate amount of PBS and mix well to obtain the icariin-bFGF-liposome complex.

Embodiment 2

[0017] Weigh 2mg of icariin, 60mg of soybean phospholipids, and 16mg of cholesterol, and dissolve them in 15ml of chloroform / methanol (2:1) mixed solvent. Place the solution in a 100ml ground-bottom flask and place it on a constant temperature water bath at 30°C. Dissolve uniformly and fully. Take the bFGF (2mg dissolved in PBS solution) solution and add it dropwise to the above solution. Ultrasound the probe for 3 seconds, with an interval of 10 seconds, and circulate 5 times. The power is 200W. Ultrasound to yellow emulsion. Rotary vacuum evaporation to remove organic The solvent appeared gelatinous at the bottom of the round-bottom flask, and continued to evaporate under reduced pressure until the gel collapsed. Add an appropriate amount of PBS and mix well to obtain the icariin-bFGF-liposome complex.

Embodiment 3

[0019] Weigh 2mg of icariin, 30mg of soybean phospholipids, and 16mg of cholesterol, and dissolve them in 15ml of chloroform / methanol (2:1) mixed solvent. Place the solution in a 100ml ground-bottom flask and place it on a constant temperature water bath at 30°C. Dissolve uniformly and fully, take bFGF (2mg dissolved in PBS solution) solution and add dropwise to the above solution, ultrasonic probe ultrasonic probe for 3s, interval 10s, cycle 5 times, power 200W, to yellow emulsion, rotary vacuum evaporation to remove The organic solvent appeared gelatinous at the bottom of the round-bottom flask, and continued to evaporate under reduced pressure until the gel collapsed. Add an appropriate amount of PBS and mix well to obtain the icariin-bFGF-liposome complex.

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PUM

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Abstract

The invention provides a method for preparing double drug-carrying liposomes. By adjusting the ratio of drugs, phospholipid and cholesterol and selecting preparation processes, the encapsulation efficiency of bFGF macromolecule proteins and icariin lipid-soluble drugs is improved, the liposomes delay the half-life period of bFGF, entrapped icariin can play a role in promoting the generation of endogenous bFGF, promoting survival of dopaminergic neurons and treating the Parkinson's disease through double drugs. The process for preparing the double drug-carrying liposomes is simple, low in cost, good in biocompatibility and non-toxic, and can be used as a nasal drug administration mode to treat the Parkinson's disease.

Description

Technical field [0001] The invention relates to a method for preparing drug-loaded liposomes, in particular to a method for preparing dual drug-loaded liposomes for treating Parkinson's disease. Background technique [0002] Parkinson’s disease is a common neurodegenerative disease. The relative hyperfunction of cholinergic nerve cells caused by striatal-nigral neurotransmitter metabolism disorder, loss of a large number of dopaminergic neurons, and decreased dopamine content is One of the main causes of Parkinson's disease. Currently, the drugs used clinically cannot effectively treat Parkinson's disease, but can only alleviate the deterioration of the disease. [0003] In the substantia nigra pathway of the midbrain, exogenous administration of basic fibroblast growth factor (bFGF) can significantly promote the survival of dopaminergic neurons, promote the migration of dopaminergic neurons, and have a good effect on Parkinson's disease. treatment effect. But bFGF has short hal...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61K9/127A61K47/28A61P25/16A61K31/7048
CPCA61K38/1825A61K9/127A61K31/7048A61K47/28A61K2300/00
Inventor 赵应征黄建平徐荷林陈翩翩朱文宗程志清陈斌杨运俊许洁
Owner 温州市中医院
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