Method for promoting function and characteristic of corneal endothelial cells

Abstract

The invention discloses a method for promoting the function and characteristic of corneal endothelial cells. The method includes the following steps that human orbit adipose-derived stem cells are separated and cultured, and a condition culture medium is extracted; primary human corneal endothelial cells are separated and cultured; the condition culture medium is added into a basic culture medium for culturing the human corneal endothelial cells to culture and proliferate the human corneal endothelial cells. The wall adherence capacity and proliferation capacity of the human corneal endothelial cells cultured by the condition culture medium extracted from the human orbit adipose-derived stem cells are high. Through tests, by the utilization of the method, the human corneal endothelial cells cultured in an in-vitro mode can do down to future ten or more generations and are amplified by more times, and the shape and functions of the human corneal endothelial cells can be kept. Animal experiments verify that by the utilization of the method, the human corneal endothelial cells cultured in the in-vitro mode have an excellent cell repairing function.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering and ophthalmic repair, and in particular relates to a method for promoting the functions and properties of corneal endothelial cells. Background technique [0002] The cornea is divided into 5 layers, from front to back: epithelial layer, Descemet's layer, stroma, Descemet's layer and endothelial layer. Among them, the innermost endothelial cell layer is of great significance for maintaining corneal transparency and its normal physiological function. Endothelial cells are a single layer of cells with a height of about 50um and a width of about 20um. Human corneal endothelial cells (Hcecs) regulate corneal transparency through pump function and barrier function. With the increase of age, the density of corneal endothelial cells gradually decreases, and when the cell density is less than 500-800 cells / mm 2 Decompensation of the corneal endothelium will result, resulting in persistent c...

AI Technical Summary

Problems solved by technology

[0004] However, the in vitro culture of corneal endothelial cells has the following disadvantages: it cannot maintain its normal cell morphology and function after multiple passages, and it cannot be applied to clinical treatment and related research.

Therefore, there is an urgent need for a suitable culture method to solve the problem that human corneal endothelial cells cannot be passaged many times and maintain cell morphology and function.

Images