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Detection method for detecting prostate cancer circulating tumor cells

A technology for prostate cancer and tumor cells, which is applied in the preparation of test samples, measuring devices, sampling, etc., can solve problems such as no detection methods, and achieve good results.

Inactive Publication Date: 2017-04-26
上海市质子重离子医院有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the deepening of the research, the results show that PSMA is expected to become a tumor marker for prostate disease monitoring with high specificity and sensitivity, but there is no good detection method yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Stain LNCaP human prostate cancer cells with antibodies according to the above method. The antibodies are prepared in antibody diluent. The concentrations of CD45-Alexa 594 antibody and PSMA-Alexa 488 antibody are both 1 mg / ml. Take 1 μl of each and add 198 μl of antibody diluent . Manual reading at room temperature, the equipment used includes low-speed large-capacity centrifuge, miniature desktop vacuum pump, miniature horizontal decolorization shaker, balance balance, electric drying oven, digital display constant temperature water bath, 50 ml large magnetic frame, manual single channel Conditioning pipettes, micro-hair dryers, large-capacity electronic pipettes, microcentrifuges, vortex mixers, in situ hybridization instruments and fluorescence microscopes, software with free scanning software for Nikon microscopes and using NIS-Elements F 4.00.00 take pictures.

[0022] The most critical judgment basis for CTC is CD45 negativity and chromosome 8 non-diploidy. CD4...

Embodiment 2

[0028] The patient's peripheral blood CTC test results and relevant medical history are as follows: the patient, male, 73 years old. The patient underwent radical prostatectomy for prostate cancer 5 years ago, and received photon radiation therapy for local recurrent prostatic fossa 2 years later, with a total dose of 68Gy / 34Fx. After radiotherapy, endocrine therapy and chemotherapy were given successively, but PSA increased progressively, in February 2016 .26 lines of SPECT (99mTc-PSMA) systemic examination showed: after prostate cancer surgery, multiple lymph nodes in the left iliac vessels and retroperitoneum were enlarged, radioactive uptake increased, and metastasis was considered; March 3, 2016 based on prostate-specific membrane antigen (PSMA) ) is a negative enrichment method for molecular markers to detect circulating tumor cells in peripheral blood. The results show that in 7.5ml of peripheral blood, a total of 15 CTCs were detected, of which 3 PSMA+ CTCs (3+, 4+, 5 ...

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Abstract

The invention discloses a detection method for detecting prostate cancer circulating tumor cells, wherein the detection method comprises the following specific steps: step one, collection of a peripheral blood sample; step two, blood cell separation; step three, antibody staining; step four, cell immobilization; step five, ethanol dehydration; step six, probe incubation; step seven, cleaning; and step eight, sheet sealing. The method can be used for early detection of prostate cancer, so as to help diagnosis and treatment; the method can specifically detect free tumor cells in a circulating blood system, calculates out the positive rate, and guides doctors to select a treatment scheme according to the positive rate and the cell number condition of patients at different time nodes of a treatment course; the method can perform subsequent detection work, such as single cell sequencing, targeted drug screening and the like, detects other tumor substances simultaneously, is more comprehensive in detection of tumor metastasis, drug resistance, differentiation and other conditions, and has good use effect.

Description

technical field [0001] The invention relates to the field of detection of peripheral blood tumor cells, in particular to a detection method for detecting circulating tumor cells of prostate cancer. Background technique [0002] Prostate cancer is one of the most common malignant tumors in men today. Its incidence rate ranks fourth among malignant tumors in the world and sixth in China, and its incidence is increasing year by year. In 2012, the incidence rate of prostate cancer in cancer registration areas in my country was 9.92 / 100,000. The onset age of prostate cancer is at a low level before the age of 55, and gradually increases after the age of 55. The incidence rate increases with age, and the peak age is 70-80 years old. It has become an important killer that seriously endangers the health of men. [0003] Circulating tumor cells (CTCs) refer to tumor cells that are released into the peripheral blood circulation from the primary tumor or metastases of solid tumors spo...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/543G01N1/28
CPCG01N33/57434G01N1/2813G01N33/54326G01N2001/2846
Inventor 傅深李萍章青陈昌岳马超张祥林
Owner 上海市质子重离子医院有限公司
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