Detection method for detecting prostate cancer circulating tumor cells
A technology for prostate cancer and tumor cells, which is applied in the preparation of test samples, measuring devices, sampling, etc., can solve problems such as no detection methods, and achieve good results.
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Embodiment 1
[0021] Stain LNCaP human prostate cancer cells with antibodies according to the above method. The antibodies are prepared in antibody diluent. The concentrations of CD45-Alexa 594 antibody and PSMA-Alexa 488 antibody are both 1 mg / ml. Take 1 μl of each and add 198 μl of antibody diluent . Manual reading at room temperature, the equipment used includes low-speed large-capacity centrifuge, miniature desktop vacuum pump, miniature horizontal decolorization shaker, balance balance, electric drying oven, digital display constant temperature water bath, 50 ml large magnetic frame, manual single channel Conditioning pipettes, micro-hair dryers, large-capacity electronic pipettes, microcentrifuges, vortex mixers, in situ hybridization instruments and fluorescence microscopes, software with free scanning software for Nikon microscopes and using NIS-Elements F 4.00.00 take pictures.
[0022] The most critical judgment basis for CTC is CD45 negativity and chromosome 8 non-diploidy. CD4...
Embodiment 2
[0028] The patient's peripheral blood CTC test results and relevant medical history are as follows: the patient, male, 73 years old. The patient underwent radical prostatectomy for prostate cancer 5 years ago, and received photon radiation therapy for local recurrent prostatic fossa 2 years later, with a total dose of 68Gy / 34Fx. After radiotherapy, endocrine therapy and chemotherapy were given successively, but PSA increased progressively, in February 2016 .26 lines of SPECT (99mTc-PSMA) systemic examination showed: after prostate cancer surgery, multiple lymph nodes in the left iliac vessels and retroperitoneum were enlarged, radioactive uptake increased, and metastasis was considered; March 3, 2016 based on prostate-specific membrane antigen (PSMA) ) is a negative enrichment method for molecular markers to detect circulating tumor cells in peripheral blood. The results show that in 7.5ml of peripheral blood, a total of 15 CTCs were detected, of which 3 PSMA+ CTCs (3+, 4+, 5 ...
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