Culture medium for rooting of tissue-cultured blueberry seedling and culturing method using same
A culture method and a technology for tissue culture seedlings are applied in the field of medium for rooting of blueberry tissue culture seedlings, which can solve the problems of affecting seedling raising efficiency, long rooting time, low rooting rate, etc., so as to improve rooting rate, strong pertinence and adaptability. Good results
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Embodiment 1
[0028] The medium for the rooting of blueberry tissue culture seedlings in this embodiment includes nutrient solution I, support I and hormones, wherein nutrient solution I is prepared with distilled water, and the nutrient solution I includes the following components and contents:
[0029] (NH 4 ) 2 SO 4 380mg / L, K 2 SO 4 450mg / L, KH 2 PO 4 170mg / L, Ca(NO 3 ) 2 .4H 2 O 150mg / L, MgSO 4 .7H 2 O 180mg / L, FeSO 4 ·7H 2 O 27.8mg / L, EDTA·Na 2 37.3mg / L, ZnSO 4 ·7H 2 O 4.3mg / L, MnSO 4 ·H 2 O 8.5mg / L, CuSO 4 .5H 2 O 0.1mg / L, Na 2 MoO 4 .2H 2 O 0.1mg / L, H 3 BO 3 6.2mg / L, VB 1 1.0mg / L, VB 6 0.5mg / L, V C 0.2mg / L, white sugar 20g / L; the pH value of the nutrient solution I is 5.2;
[0030] The support I is perlite and agar, the content of perlite in the above nutrient solution I is 60g / L, and the content of agar in the above nutrient solution I is 3.5g / L;
[0031] The hormone is IBA, and the content of this hormone in the nutrient solution I is 0.2mg / L.
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Embodiment 2
[0041] The medium for the rooting of blueberry tissue culture seedlings in this embodiment includes nutrient solution I, support I and hormones, wherein nutrient solution I is prepared with distilled water, and the nutrient solution I includes the following components and contents:
[0042] (NH 4 ) 2 SO 4 375mg / L, K 2 SO 4 445mg / L, KH 2 PO 4 165mg / L, Ca(NO 3 ) 2 .4H 2 O 145mg / L, MgSO 4 .7H 2 O 175mg / L, FeSO 4 ·7H 2 O 27mg / L, EDTA Na 2 37mg / L, ZnSO 4 ·7H 2 O 4mg / L, MnSO 4 ·H 2 O 8mg / L, CuSO 4 .5H 2 O 0.05mg / L, Na 2 MoO 4 .2H 2 O 0.05mg / L, H 3 BO 3 6mg / L, VB 1 0.5mg / L, VB 6 0.2mg / L, V C 0.1mg / L, white sugar 20g / L; the pH value of the nutrient solution I is 5.3;
[0043] The support I is perlite and agar, the content of perlite in the above nutrient solution I is 55g / L, and the content of agar in the above nutrient solution I is 4g / L;
[0044] The hormone is IBA, and the content of this hormone in nutrient solution Ⅰ is 0.05 mg / L.
[0045] The cu...
Embodiment 3
[0054] The medium for the rooting of blueberry tissue culture seedlings in this embodiment includes nutrient solution I, support I and hormones, wherein nutrient solution I is prepared with distilled water, and the nutrient solution I includes the following components and contents:
[0055] (NH 4 ) 2 SO 4 385mg / L, K 2 SO 4 455mg / L, KH 2 PO 4 175mg / L, Ca(NO 3 ) 2 .4H 2 O 155mg / L, MgSO 4 .7H 2 O185mg / L, FeSO 4 ·7H 2 O28mg / L, EDTA·Na 2 38mg / L, ZnSO 4 ·7H 2 O 4.5mg / L, MnSO 4 ·H 2 O9mg / L, CuSO 4 .5H 2 O 0.15mg / L, Na 2 MoO 4 .2H 2 O 0.1mg / L, H 3 BO 3 6.5mg / L, VB 1 1.5mg / L, VB 6 0.6mg / L, V C 0.3mg / L, white sugar 22g / L; the pH value of the nutrient solution I is 5.5;
[0056] The support I is perlite and agar, the content of perlite in the above nutrient solution I is 65g / L, and the content of agar in the above nutrient solution I is 3g / L;
[0057] The hormone is IBA, and the content of this hormone in nutrient solution I is 0.5 mg / L.
[0058] The cult...
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