A method for rapid cultivation of small commercial pot plants of the genus Echeveria which is difficult to propagate by cuttings in a bottle
A technology of cutting propagation and imitation stone lotus, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of uneven quality of reorganized or plastic potted plants, low effective seedling rate, and difficulty in seedling breeding, etc. Achieve the effect of excellent product properties, beautiful shape, and neat seedling age
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Embodiment 1
[0031] (1) Induction and cultivation of high-quality plastic rooted bush seedlings
[0032] ① Selection and disinfection of explants
[0033] Using the imported Echeveria 'Colorate Special', a high-end famous product of the genus Echeveria, as the material, the detached leaves of the genus Echeveria without pests and diseases or damage were selected as explants, and dried overnight in a cool and dry place at room temperature. Two days after washing once with 1% v / v detergent aqueous solution, rinse it under tap water for 0.5h, drain the surface water, place it on a super-clean workbench, soak it in 75% v / v alcohol for 12s, Then soak in 0.05% w / w mercuric chloride solution with Tween-20 for 5 minutes, wash with sterile water for 3 minutes, and finally soak with 2.5% w / w sodium hypochlorite solution with Tween-20 for 3 minutes, wash with sterile water 4 times, each Wash for 3 minutes to obtain sterile detached leaves. Under aseptic conditions, cut off the middle and upper tissu...
Embodiment 2
[0050] Embodiment 2 is the same as Embodiment 1 except that the following steps are different.
[0051] (1) Induction and cultivation of high-quality plastic rooted bush seedlings
[0052] ① Selection and disinfection of explants
[0053] Take the high-end famous product of Echeveria 'Agavoides Red' as the material, soak in 75% v / v alcohol for 15s, and use 0.05% w / w mercuric chloride added with Tween-20 Soak in the solution for 6 minutes, soak in 2.5% w / w sodium hypochlorite solution with Tween-20 for 4 minutes, then wash with sterile water for 5 times, each time for 3 minutes, to obtain sterile leaves in vitro, and remove the leaves under aseptic conditions In the upper part of the tissue, the leaves from the base of the leaves to the top, with a length of 1.5cm to 1.8cm, containing the axillary bud meristems at the base of the leaves, are kept as sterile induced explants, and are set aside; the 0.05% w / w of Tween-20 is added The mercuric chloride solution is 0.05% w / w merc...
Embodiment 3
[0068] Embodiment 3 is the same as Embodiment 1 except that the following steps are different.
[0069] (1) Induction and cultivation of high-quality plastic rooted bush seedlings
[0070] ① Selection and disinfection of explants
[0071] Use the high-end famous product "Echeveria 'Elegance'" of the genus Echeveria, which is difficult to propagate by cuttings, as the material. After washing once with an aqueous solution of detergent with a concentration of 1% v / v, rinse it under tap water for 1.0h, 75% Soak in v / v alcohol for 12s, soak in 0.05% w / w mercuric chloride solution added with Tween-20 for 5 minutes, wash with sterile water for 2 minutes, soak in 2.5% w / w sodium hypochlorite solution added with Tween-20 for After that, wash 5 times with sterile water for 2 minutes each time to obtain sterile isolated leaves. Under aseptic conditions, cut off the middle and upper parts of the leaves, and keep the leaves from the base to the top, with a length of 2.0cm to 2.3cm. The l...
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