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A method for rapid cultivation of small commercial pot plants of the genus Echeveria which is difficult to propagate by cuttings in a bottle

A technology of cutting propagation and imitation stone lotus, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of uneven quality of reorganized or plastic potted plants, low effective seedling rate, and difficulty in seedling breeding, etc. Achieve the effect of excellent product properties, beautiful shape, and neat seedling age

Active Publication Date: 2018-08-14
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the difficulties in seedling breeding of the above-mentioned famous and precious varieties of the genus Pseudomonas, the effective seedling rate is low, the seedling cycle is long, and the conventional breeding seedlings need to be reorganized or reshaped after they come out of the nursery, and the quality of the seedlings and potted plants is uneven, and the production High cost and other defects, the present invention provides a method for quickly cultivating small potted plants of the genus Echeveria in a bottle that is difficult to propagate by cuttings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Induction and cultivation of high-quality plastic rooted bush seedlings

[0032] ① Selection and disinfection of explants

[0033] Using the imported Echeveria 'Colorate Special', a high-end famous product of the genus Echeveria, as the material, the detached leaves of the genus Echeveria without pests and diseases or damage were selected as explants, and dried overnight in a cool and dry place at room temperature. Two days after washing once with 1% v / v detergent aqueous solution, rinse it under tap water for 0.5h, drain the surface water, place it on a super-clean workbench, soak it in 75% v / v alcohol for 12s, Then soak in 0.05% w / w mercuric chloride solution with Tween-20 for 5 minutes, wash with sterile water for 3 minutes, and finally soak with 2.5% w / w sodium hypochlorite solution with Tween-20 for 3 minutes, wash with sterile water 4 times, each Wash for 3 minutes to obtain sterile detached leaves. Under aseptic conditions, cut off the middle and upper tissu...

Embodiment 2

[0050] Embodiment 2 is the same as Embodiment 1 except that the following steps are different.

[0051] (1) Induction and cultivation of high-quality plastic rooted bush seedlings

[0052] ① Selection and disinfection of explants

[0053] Take the high-end famous product of Echeveria 'Agavoides Red' as the material, soak in 75% v / v alcohol for 15s, and use 0.05% w / w mercuric chloride added with Tween-20 Soak in the solution for 6 minutes, soak in 2.5% w / w sodium hypochlorite solution with Tween-20 for 4 minutes, then wash with sterile water for 5 times, each time for 3 minutes, to obtain sterile leaves in vitro, and remove the leaves under aseptic conditions In the upper part of the tissue, the leaves from the base of the leaves to the top, with a length of 1.5cm to 1.8cm, containing the axillary bud meristems at the base of the leaves, are kept as sterile induced explants, and are set aside; the 0.05% w / w of Tween-20 is added The mercuric chloride solution is 0.05% w / w merc...

Embodiment 3

[0068] Embodiment 3 is the same as Embodiment 1 except that the following steps are different.

[0069] (1) Induction and cultivation of high-quality plastic rooted bush seedlings

[0070] ① Selection and disinfection of explants

[0071] Use the high-end famous product "Echeveria 'Elegance'" of the genus Echeveria, which is difficult to propagate by cuttings, as the material. After washing once with an aqueous solution of detergent with a concentration of 1% v / v, rinse it under tap water for 1.0h, 75% Soak in v / v alcohol for 12s, soak in 0.05% w / w mercuric chloride solution added with Tween-20 for 5 minutes, wash with sterile water for 2 minutes, soak in 2.5% w / w sodium hypochlorite solution added with Tween-20 for After that, wash 5 times with sterile water for 2 minutes each time to obtain sterile isolated leaves. Under aseptic conditions, cut off the middle and upper parts of the leaves, and keep the leaves from the base to the top, with a length of 2.0cm to 2.3cm. The l...

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PUM

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Abstract

The invention discloses a method for quickly cultivating small potted Echeveria plant goods difficult to cut and propagate on the basis of in-bottle molding. The small potted plant goods are obtained through two steps. The method includes step 1, inducing and cultivating high-quality molded rooting seedlings, and step 2, transplanting the molded rooting seedlings to pots for acclimation, and cultivating the small potted plant goods according to standards in later period. The step 1 includes selection and sterilization of explants, induction of regeneration bud seedlings, molding, proliferation and cultivation of the regeneration bud seedlings, secondary molding and rooting of strong seedlings. The method has the advantages that the technical problems of seeding propagation difficulty of the small potted Echeveria plant goods difficult to cut and propagate, low effective seed feeding rate, recombination or shape modification after single sprout potting, long operation cycle and the like are solved; effective seed feeding rate of tissue culture reaches more than 89%, duration is shortened by 90-100 days as compared with conventional methods, cost is reduced, and uniformity of potted plants for sale is high; particularly, the method has high economic benefit and practical utilization value in development of high-end famous Echeveria seedling products which are difficult to obtain through field cutting.

Description

technical field [0001] The invention discloses a method for quickly cultivating commercial small potted plants of the genus Echeveria that are difficult to propagate by cutting and propagating by plastic molding in a bottle, and belongs to the technical field of tissue culture rapid propagation and cultivation. Background technique [0002] Crassulaceae Echeveria is a perennial succulent ornamental herb, native to Pakistan, which can be cultivated in many places in the world. It has a beautiful appearance and rich colors. In the past two years, large-scale planting has developed rapidly. The rapid increase, the supply and marketing of commercial potted plants are booming. Especially since 2015, the demand for commercial potted plants of imported high-end varieties such as Echeveria 'Colorate Special', Echeveria 'Agavoides Red' and Echeveria 'Elegance' has increased rapidly, and the prices have remained high On the one hand, the market prospect and economic value are broad. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 王丽花瞿素萍吴学尉王继华张艺萍杨秀梅张丽芳苏艳许凤
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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