Anthocyanin lipidosome lyophilized powder and preparation method thereof

A technology of freeze-dried powder and liposome, applied in the biological field, can solve the problems of low bioavailability, unstable liposome properties, and single type of wall material, and achieve high bioavailability, simple method, and good stability Effect

Active Publication Date: 2017-05-10
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the liposomes are now prepared by using natural phospholipids and cholesterol to compound as wall materials according to a certain ratio, but the resulting liposomes are unstable in nature, low in bioavailability, and the wall materials are of a single type.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] Preparation of mannose erythritol lipid MEL-A:

[0035] MELs are fermented and synthesized by bacteria of the genus Ustligo maydis or Pseudazyma, and then separated and purified to obtain mannose erythritol lipid MEL-A with a purity of more than 95%. Specifically include the following steps:

[0036] The strain Pseudozyma aphidis DSMZ70725 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen; Ustligo maydis (No. 5.203) was purchased from China General Microbiological Culture Collection Center (CGMCC). Transfer one of the above purchased strains to slant medium (3.0g / L yeast extract, 3.0g / L malt extract, 5.0g / L peptone, 10.0g / L glucose, 15.0g / L agar, distilled water) , activated at 28°C for 2 days, and then transferred to liquid medium (4.0g / L glucose, 3.0g / L NaNO 3 , 0.3g / L MgSO4 ·7H 2 O, 0.3g / L KH 2 PO 4 , 1.0g / L yeast extract, distilled water, pH 6.0), cultured at 28°C and 220rpm for 3d. Insert the cultured seed liquid into the fermentation ...

Embodiment 1

[0047] A preparation method of anthocyanin liposome freeze-dried powder, comprising the following steps:

[0048] (1) Dissolving the prepared mannose erythritol lipid MEL-A in n-hexane to obtain a solution with a concentration of 15.0 mg / L;

[0049] (2) dissolving anthocyanins in distilled water to obtain a solution B with a concentration of 100.0 mg / L;

[0050] (3) Mix the A solution obtained in step (1) and the B solution obtained in step (2) according to a volume ratio of 1:1, ultrasonically treat it at 30°C with a power of 100W for 5 minutes, and let it stand for 15 minutes; then place Evaporate under reduced pressure in a rotary evaporator (vacuum degree is 0.1MPa, rotation speed is 120rpm, temperature is 55°C) to obtain lipid film;

[0051] (4) adding pH 5.0 to 7.0 and phosphate buffer solution with an ion concentration of 0.05 to 0.1M to the lipid film obtained in step (3), stirring and dissolving and passing through a 0.22 μm filter membrane to obtain a liposome suspe...

Embodiment 2

[0055] A preparation method of anthocyanin liposome freeze-dried powder, comprising the following steps:

[0056] (1) Dissolving the prepared mannose erythritol lipid MEL-A in chloroform to obtain a solution A with a concentration of 20.0 mg / L;

[0057] (2) dissolving anthocyanins in distilled water to obtain a solution B with a concentration of 300.0 mg / L;

[0058] (3) Mix the A solution obtained in step (1) and the B solution obtained in step (2) according to a volume ratio of 3:1, ultrasonically treat it at 40°C with a power of 300W for 10 minutes, and let it stand for 20 minutes; then place Evaporate under reduced pressure in a rotary evaporator (vacuum degree is 0.1MPa, rotation speed is 100rpm, temperature is 45°C) to obtain lipid film;

[0059] (4) adding pH 5.0 to 7.0 and phosphate buffer solution with an ion concentration of 0.05 to 0.1M to the lipid film obtained in step (3), stirring and dissolving and passing through a 0.45 μm filter membrane to obtain a liposome ...

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Abstract

The invention discloses novel anthocyanin lipidosome lyophilized powder. The novel anthocyanin lipidosome lyophilized powder is prepared from mannosylerythritol lipid or a mannosylerythritol lipid / phospholipid mixture as a wall material. A specific preparation method comprises following steps: (1), mannosylerythritol lipid is dissolved in an organic solvent, and a solution A is obtained; (2), anthocyanin is dissolved in a solvent, and a solution B is obtained; (3), the solution A obtained in the step (1) and the solution B obtained in the step (2) are mixed uniformly, subjected to ultrasonic treatment and left to stand for balance, a mixture is put in a rotary evaporator and subjected to reduced-pressure evaporation, and a lipid membrane is obtained; (4), a phosphate buffer solution is added to the lipid membrane obtained in the step (3), membrane filtration is performed after stirring and dissolving, and a lipidosome suspension is obtained; (5), a lyophilized protective agent is added to the lipidosome suspension obtained in the step (4), and the novel anthocyanin lipidosome lyophilized powder is obtained through lyophilization. The prepared anthocyanin lipidosome is good in stability; the preparation method is simple and low in cost; the preparation wall material of the lipidosome is enriched, and the novel anthocyanin lipidosome lyophilized powder can be applied to the fields of food and cosmetics.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a novel anthocyanin liposome freeze-dried powder and a preparation method thereof. Background technique [0002] Anthocyanin is a safe and non-toxic natural food coloring, which is rich in sources and has high nutritional and pharmacological value, and has great application potential in the fields of food, cosmetics and medicine. However, due to the poor stability, easy degradation and low bioavailability of anthocyanins, their wide application is limited. In recent years, the steady-state technology of anthocyanins, especially the liposome preparation technology has become a research hotspot in this field. By embedding the anthocyanin in the liposome, it can not only prevent the inactivation of the anthocyanin in contact with water molecules in space, but also stabilize the biological activity of the anthocyanin, improve the release speed of the anthocyanin, and increas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/60A61K8/49A61K8/14A61Q19/00A23L5/40
CPCA61K8/14A61K8/498A61K8/602A61K2800/85A61Q19/00
Inventor 范琳琳周剑忠夏秀东王英李莹刘小莉黄自苏
Owner JIANGSU ACAD OF AGRI SCI
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