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Applications of picroside II in treating cerebral arterial thrombosis

A technology of ischemic stroke and berberine, which is applied in the application field of berberine II to treat ischemic stroke, to improve neurological dysfunction, reduce cerebral cortex neuron structural damage, and achieve excellent therapeutic effects

Inactive Publication Date: 2017-05-10
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At this stage, scholars at home and abroad have reported some patents (CN012125544.X) on the application of berberine II in the prevention and treatment of allergic inflammatory diseases, and the patent (CN200410098646.4) on the application of berberine II in the treatment of hepatitis B , but there is no patent report on the treatment of berberine II in the treatment of brain damage caused by ischemic stroke

Method used

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  • Applications of picroside II in treating cerebral arterial thrombosis
  • Applications of picroside II in treating cerebral arterial thrombosis
  • Applications of picroside II in treating cerebral arterial thrombosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The mNSS method was used to evaluate the effect of berberine II on the neurobehavioral function of cerebral ischemia-reperfusion rats, and the specific steps were as follows:

[0027] Step 1: The middle cerebral artery occlusion (MCAO) model was established by using the suture method through the left external carotid-internal carotid artery. Fix the laser Doppler blood flow detector (PE-5001, Swedan) probe on the dura mater through the bone hole, record the blood flow changes in the blood supply area of ​​the left middle cerebral artery, and when the blood flow becomes stable, pass through the left The external carotid-internal carotid artery thread is inserted, and the thread plug is pulled out after 2 hours of ischemia to achieve blood reperfusion, so that the peak value of regional cerebral blood flow (rCBF) after the thread plug is inserted is reduced to 30% or less of that before thread insertion, and the thread is pulled out After embolization, the rCBF recovered ...

Embodiment 2

[0040] TTC staining was used to detect the effect of berberine II on the volume of cerebral infarction in rats with cerebral ischemia-reperfusion. The specific steps are as follows:

[0041] TTC (2,3,5-triphenyltetrazolium chloride) staining was used to observe the effect of berberine II on cerebral infarct volume in MCAO rats. TTC itself can be used as a redox indicator, and dehydrogenases in living cells (especially succinate dehydrogenase in mitochondria) can reduce TTC to the red formazan compound TPF (1,3,5-triphenylformazan), The ischemic infarcted tissue is pale due to the loss of dehydrogenase activity due to tissue necrosis, while the normal tissue is dark red. After 22 hours of cerebral ischemia-reperfusion, rats were deeply anesthetized with 10% volume concentration of chloral hydrate, and five slices of 2mm thick brain coronal slices were taken. Incubate for 10 min in the dark in an incubator. TTC staining results as figure 1 As shown, the brain tissue of the ra...

Embodiment 3

[0043] Test the effect of berberine II on the blood-brain barrier (BBB) ​​of cerebral ischemia-reperfusion rats, the specific steps are as follows:

[0044] (1) Test the brain water content to evaluate the effect of pupagoside II on the degree of cerebral edema in cerebral ischemia-reperfusion rats:

[0045] Establish the MCAO model of rats. After 22 hours of cerebral ischemia and reperfusion, quickly decapitate and take the brain, separate the left and right hemispheres along the sagittal suture, take the left hemisphere, absorb the surface water with filter paper, weigh the wet weight, and bake in an oven at 110°C After 24 hours to constant weight, take it out and weigh the dry weight immediately, and calculate the brain water content: (wet weight-dry weight) / wet weight*100%. The results of brain water content are shown in Table 2. The brain water content of rats in the model group was significantly higher than In the sham operation group (P0.05);

[0046] Table 2 Brain wat...

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Abstract

The invention belongs to the field of medicine and particularly relates to applications of picroside II in treating cerebral injury caused by cerebral arterial thrombosis. Rats with middle cerebral artery ischemia as models, picroside II is used for performing intervention treatment and has significant effects of reducing cerebral infraction volume, improving the morphology and structure of brain tissue in ischemic areas and improving neurobehavioral dysfunction of the rats. The significant effects of the picroside II on oxidation resistance, brain tissue protection and blood brain barrier protection are equivalent to that of apocynin, and the neurobehavioral function improvement of the picroside II is also equivalent to that of apocynin. The applications of picroside II have a neuroprotective effect on cerebral ischemia reperfusion injury, and by regulating and controlling the Rac1 / Nox2 / ROS / ROCK / Claudin 5 signal pathways, cerebral injury and neurological dysfunction caused by cerebral ischemia reperfusion injury are improved, and therefore the picroside II is expected to be developed into a new drug for treating cerebral ischemia reperfusion injury.

Description

[0001] Technical field: [0002] The invention belongs to the field of drug research and development, and in particular relates to the application of berberine II in treating ischemic stroke. [0003] Background technique: [0004] Cerebral stroke is a common cerebrovascular accident, including ischemic and hemorrhagic stroke. There are 1.5 million to 1.8 million new stroke patients in my country every year, and cerebrovascular disease ranks first among the three major causes of death. It has the characteristics of high morbidity, high mortality, high disability rate, and high recurrence rate. Stroke refers to the stenosis, occlusion or rupture of cerebral arteries caused by various predisposing factors, resulting in acute cerebral blood circulation disorder, clinically manifested as symptoms and signs of transient or permanent brain dysfunction, acute ischemic cerebral Stroke is the most common type of stroke, accounting for about 60%-80% of all strokes. Ischemic stroke is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P9/10
CPCA61K31/7048
Inventor 郭云良翟丽王婷婷季亚清张红艳李珊赵丽徐颖婕郝翠张丽娟
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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