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Function and application of RGS10 (regulator of G-protein signaling 10) in treatment of fatty liver and type-II diabetes

A diabetes, 1. RGS10 technology, applied in gene therapy, metabolic diseases, biochemical equipment and methods, etc., can solve problems such as unclear effects

Active Publication Date: 2017-05-10
武汉惠康基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its role in fatty liver and type 2 diabetes is still unclear and further research is needed

Method used

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  • Function and application of RGS10 (regulator of G-protein signaling 10) in treatment of fatty liver and type-II diabetes
  • Function and application of RGS10 (regulator of G-protein signaling 10) in treatment of fatty liver and type-II diabetes
  • Function and application of RGS10 (regulator of G-protein signaling 10) in treatment of fatty liver and type-II diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] [Example 1] Obtaining mouse fatty liver and type II diabetes model (diet induced obesity, DIO)

[0057] (1) Grouping of experimental animals: 8-week-old, male, WT mice and RGS10-KO mice were selected and given two special diets, D12942 high-fat diet (High fat diet, HFD) and D12450B low-fat diet (Normal chow , NC) feeding, that is, WT NC group, KO NC group, WT HFD group, KO HFD group, a total of 4 groups.

[0058] (2) The model induces the operation process through high-fat feed:

[0059] Using WT and KO mice, the DIO model was established, and phenotype correlation analysis was performed to clarify the role of RGS10 gene on fatty liver and type II diabetes. 8-week-old, male, WT mice and RGS10-KO (TG) mice were selected and fed with two special diets, D12942 high-fat diet (Highfat diet, HFD) and D12450B low-fat diet (Normal chow, NC), respectively. That is WTNC group, KO NC group, WT HFD group, KO HFD group, a total of 4 groups. The food intake of the mice was recorde...

Embodiment 2

[0060] [Example 2] Determination of mouse body weight and blood sugar level

[0061] (1) Detection of fasting body weight of mice

[0062] 1) Weight detection.

[0063] ①Fasting: fast the mice to be tested at 8:00 am (without water), and start the experimental operation at 2:00 pm.

[0064] ② Weighing: Weigh at 0 week, 2 weeks, 4 weeks, 6 weeks, 8 weeks, 10 weeks, and 12 weeks respectively. In a small bucket, measure the weight and record the data. Feed amount detection: After the weighing operation is completed, add feed to the mice, and record the amount of feed for the mice on the dynamic electronic balance.

[0065] (2) Fasting blood glucose level detection experiment

[0066] All the mice to be tested were fasted from 8:00 am to 2:00 pm (without water), that is, the experimental operation was started after 6 hours of fasting.

[0067] ① Blood glucose meter preparation: Check the battery of the blood glucose meter (Johnson & Johnson, ONETOUCH), press the switch on the...

Embodiment 3

[0072] [Example 3] Glucose tolerance test (intraperitoneal glucose tolerance test, IPGTT)

[0073] In the 11th week of the experiment, the intraperitoneal injection of glucose test (IPGTT) was performed to evaluate the glucose tolerance of the mice.

[0074] (1) Before measuring blood glucose, measure the fasting body weight of the mice, and calculate the injection volume of glucose based on 10 μL / g.

[0075] (2) First detect the fasting blood glucose at 0 minutes before the glucose injection, and inject the glucose solution intraperitoneally quickly after the detection is completed.

[0076] (3) Operation method of intraperitoneal injection: ①Fix the mouse; grab the mouse, grab the tail of the mouse with the little finger and ring finger of the left hand, and grab the neck of the mouse with the other three fingers, so that the head of the mouse is downward, and the The abdomen of the mouse is fully exposed. ②Needle positioning and injection: insert the needle from the side ...

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Abstract

The invention discloses a function and an application of RGS10 (regulator of G-protein signaling 10) gene in fatty liver and diabetes. An RGS10 gene knockout mouse and a WT (wild type) C57 mouse re used as experimental subjects, the function of the RGS10 gene is studied by the aid of an HFD (high fat diet) induced obesity mouse model, and the result indicates that compared with the WT G57 mouse, the RGS10-KO mouse shows obesity and has the fasting blood glucose level higher than that of the WT mouse in a control group and the liver function obviously poorer than that of the WT mouse. An intraperitoneal injection glucose tolerance experiment finds that the tolerance capacity of the RGS10-KO mouse is obviously weakened. The liver weight, liver / weight ratio as well as lipid component and glycogen content pathological staining results indicate that fatty liver lesion of the RGS10-KO mouse in an HFD group is obviously aggravated and lipid accumulation is significantly increased. Therefore, the RGS10 can be used as a drug target for screening and treating the fatty liver and / or type-II diabetes. An RGS10 accelerator can be used for preparing drugs for treating the fatty liver and / or type-II diabetes.

Description

technical field [0001] The present invention belongs to the field of gene function and application, and particularly relates to the function and application of a G protein signal transduction regulatory protein 10 (Regulator of G-protein signaling 10, RGS10) in the treatment of fatty liver and type II diabetes, specifically in the It is used in the preparation of medicaments for preventing, alleviating and / or treating fatty liver and / or type II diabetes. Background technique [0002] In recent years, with the continuous development of my country's economy, the average life expectancy has increased year by year, and the incidence of chronic non-communicable diseases such as fatty liver and type 2 diabetes has increased significantly. According to statistics, among people over 20 years old in my country, the prevalence rate of type Ⅱ diabetes is 9.7%, which is the most common chronic endocrine system disease. It is caused by various factors such as genetic factors, immune dys...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K48/00A61P1/16A61P3/08A61P3/10C12Q1/02
CPCA61K48/005A61K49/0008G01N33/5008
Inventor 李红良王丕晓
Owner 武汉惠康基因科技有限公司
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