Method for extracting nobiletin and tangeretin from citrus

A technology of tangerine peel and orange peel, which is applied in the field of plant extraction, can solve the problems of low extraction efficiency, single extraction components, and many elution times, and achieves the effects of improving extraction rate, simplifying technological process and reducing cost.

Inactive Publication Date: 2017-05-10
YAAN TAISHI BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a method for extracting nobiletin and tangeretin from citrus, which solves the problems of single extraction components and low extraction efficiency caused by many elution times in the prior art

Method used

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  • Method for extracting nobiletin and tangeretin from citrus
  • Method for extracting nobiletin and tangeretin from citrus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The raw orange peel is produced in Jintang County, Sichuan. The orange peel is dried until the water content is lower than 10%, and then crushed and passed through a 30-mesh sieve.

[0051] Take 200g orange peel powder, add 1200mL 70% ethanol aqueous solution, then adjust pH=8 with 5% NaoH solution; reflux extraction at 80°C for 2 hours, filter and collect the filtrate; Reflux extraction at 80°C for 2 times, 1 hour each time. The three filtrates were combined to a total of 3000 mL. Recover ethanol with a rotary evaporator to obtain 450 mL of citrus flavonoid concentrate.

[0052] The concentrated solution of citrus flavonoids was extracted by adding ethyl acetate at a ratio of 1:3 for 3 times, each time for one hour, and the aqueous phase and the organic phase were separated; after the ethyl acetate was recovered from the organic phase, there was about 30 mL of concentrated solution of methoxylated flavones. Use a constant flow pump to pump ethyl acetate into the medi...

Embodiment 2

[0055] The raw orange peel is produced in Jintang County, Sichuan. The orange peel is dried until the water content is lower than 10%, and then crushed and passed through a 30-mesh sieve.

[0056] Take 200g of orange peel powder and add 5g of quicklime and mix evenly, then add 1200mL of 50% ethanol aqueous solution, reflux extraction at 80°C for 2 hours, filter and collect the filtrate; 1 hour each time. The three filtrates were combined to a total of 2900 mL. Recover ethanol with a rotary evaporator to obtain 400 mL of citrus flavonoid concentrate.

[0057] The concentrated solution of citrus flavonoids was extracted by adding ethyl acetate at a ratio of 1:3 for 3 times, each time for one hour, and the aqueous phase and the organic phase were separated; after the ethyl acetate was recovered from the organic phase, there was about 30 mL of concentrated solution of methoxylated flavones. Use a constant flow pump to pump ethyl acetate into the medium-pressure chromatography co...

Embodiment 3

[0060] The raw orange peel is produced in Jintang County, Sichuan. The orange peel is dried until the water content is lower than 10%, and then crushed and passed through a 30-mesh sieve.

[0061] Take 200g orange peel powder, add 1g potassium hydroxide, then add 1200mL 80% methanol aqueous solution, reflux extraction at 75°C for 2 hours, filter and collect the filtrate; Reflux extraction 2 times, 1 hour each time. The combined filtrates totaled 2900 mL. Recover ethanol with a rotary evaporator to obtain 400 mL of citrus flavonoid concentrate.

[0062] The citrus flavonoids concentrate was extracted by adding n-hexane at a ratio of 1:3 for 3 times, each time for one hour, and the aqueous phase and the organic phase were separated; after recovering the n-hexane from the organic phase, there was about 30 mL of the concentrated methoxylated flavones. Use a constant flow pump to pump n-hexane into the medium-pressure chromatographic column until the solvent flows out, pump the p...

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Abstract

The invention provides a method for extracting nobiletin and tangeretin from citrus. The method for extracting the nobiletin and the tangeretin from the citrus includes following steps: step A, using a mixed solution of first organic solvent and water as an extracting agent, controlling pH (potential of hydrogen) value of the solution at 8-9 to perform reflux extraction on extracted material once or more than once, mixing and concentrating extraction solutions to obtain a tangeritin concentrated solution; step B, using second organic solvent for extracting the tangeritin concentrated solution, and separating an organic phase and a water phase out; step C, removing the extracting agent out of the organic phase, and then sequentially using first eluent and second eluent for elution in macroporous adsorption resin, and respectively collecting the nobiletin and the tangeretin. The method for extracting the nobiletin and the tangeretin from the citrus solves the problem that extraction efficiency is low caused by single extracted components and repeated elution in the prior art.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting nobiletin and tangeretin from oranges. Background technique [0002] Citrus (Citrus reticulata Blanco), a plant belonging to Rutaceae, citrus peel has high medicinal value, and orange peel also contains thiamine, riboflavin, volatile oil, and flavonoids. The volatile oils in orange peel can irritate the digestive tract. It can increase the secretion of gastric juice, promote gastrointestinal peristalsis, invigorate the stomach and expel wind. Flavonoids have the effect of dilating coronary arteries and increasing coronary blood flow. In addition, orange peel also has anti-inflammatory, anti-ulcer, antibacterial and choleretic effects. Vitamin P in orange peel has a significant effect on vascular sclerosis. [0003] Among them, nobiletin and tangeretin are the main parts of citrus polymethoxyflavonoids (PMFs), although their content in citrus p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 郭军伟梁林辉宋进博李海媚杨军陈彬王顺尧
Owner YAAN TAISHI BIOLOGICAL SCI & TECH CO LTD
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