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Extraction method of rubusoside

An extraction method and rubaside technology, which are applied in the field of chemical extraction, can solve the problems of few reports on the industrial production of rubaside, and achieve the effects of being beneficial to leaching and separation, speeding up extraction and improving product purity.

Inactive Publication Date: 2017-05-10
广西金秀香料香精有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The separation, extraction, testing and pharmacology of rubusoside have been studied at home and abroad, and there are also reports on the small-scale extraction of rubusoside with higher purity, but there are not many reports on the industrial production of rubusoside with high content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Wash 500g of sweet tea, crush it to 50-100 mesh, add 4000g of hydrochloric acid and sodium dihydrogen phosphate buffer solution, the pH is 5.0, and add cellulase, ultrasonically extract at 40°C for 60min, after the extraction is completed 90 Enzyme inactivation at ℃ for 10 minutes, centrifuged, and the filter residue was extracted for the second time, extracted with 85°C pure water at 4500g, ultrasonically extracted for 70 minutes, centrifuged, the two extracts were combined and concentrated, and then placed in the microwave together with the filter residue In the extraction device, the microwave power is 300W, the temperature is 50°C, and the ethanol solution with a volume fraction of 90% is used to extract for 30 minutes, centrifugally filtered, the extract is concentrated under reduced pressure, and dissolved in an acetone solution with a volume fraction of 70%. Elution is carried out through AB-8 macroporous resin column, and the eluate is collected and concentrated ...

Embodiment 2

[0028] Wash 500g of sweet tea, crush it to 50-100 mesh, add 5000g of hydrochloric acid and sodium dihydrogen phosphate buffer solution, the pH is 5.0, and add cellulase, ultrasonically extract at 35°C for 70min, after the extraction is completed Perform enzyme inactivation at 90°C for 10 minutes, centrifuge, and extract the filter residue for the second time. The extract is 5000g of 70°C pure water, ultrasonically extract for 60 minutes, centrifuge, combine the two extracts and concentrate, and then put them together with the filter residue In the microwave extraction device, the microwave power is 350W, the temperature is 70°C, and the ethanol solution with a volume fraction of 80% is used for extraction for 30 minutes, centrifugally filtered, the extract is concentrated under reduced pressure, and dissolved in an acetone solution with a volume fraction of 70%. The solution is eluted through a HPD400 type macroporous resin column, and the eluate is collected and concentrated i...

Embodiment 3

[0030]Wash 500g of sweet tea, crush it to 50-100 mesh, add 4500g of hydrochloric acid and sodium dihydrogen phosphate buffer solution, the pH is 5.0, and add cellulase, ultrasonically extract at 30°C for 65min, after the extraction is completed 90 Enzyme inactivation at ℃ for 10 minutes, centrifuged, and the filter residue was extracted for the second time, the extract was 4000g of 85°C pure water, ultrasonically extracted for 70 minutes, centrifuged, the two extracts were combined and concentrated, and then placed together with the filter residue In the microwave extraction device, the microwave power is 400W, the temperature is 30°C, and the ethanol solution with a volume fraction of 70% is used to extract for 40 minutes, centrifugally filtered, and the extract is concentrated under reduced pressure, and then dissolved with an acetone solution with a volume fraction of 70%. The solution was eluted through an X-5 macroporous resin column, and the eluate was collected and conce...

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PUM

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Abstract

The invention discloses an extraction method of rubusoside. The method comprises the following steps of crushing leaves of strigose hydrangea; performing extraction by a buffer solution; performing cellulase hydrolysis; after the extraction at 30 to 40 DEG C, performing enzyme inactivation; then, performing water extraction at 70 to 90 DEG C; after the extraction liquid is concentrated, performing ethanol microwave extraction for filter slag; after the extraction liquid concentration, performing chromatography through macroporous resin; using acetone as an eluent; collecting the eluent to be concentrated into extract; performing polyamide Column purification; firstly performing water leaching impurity removal; then, performing ethanol leaching impurity removal by the ethanol with the volume percentage being 10 to 30 percent; finally, performing elution by ethanol with the volume percentage being 50 to 70 percent; collecting eluate; performing spraying drying to obtain the rubusoside extracts. The method provided by the invention has the advantages that the extraction time is shortened; the efficiency is improved; the extraction rate of the extracted rubusoside product is not lower than 80 percent; the purity is not lower than 99 percent; the crystals are pure white crystals, and belongs to high-content and high-quality extracts of leaves of strigose hydrangea; no bitter taste exists; the sweetness is high; the requirements on high-quality rubusoside products in the market can be met.

Description

technical field [0001] The invention relates to the technical field of chemical extraction, in particular to a rubusoside extraction method. Background technique [0002] Guangxi sweet tea Rubus Suatrssimus S. Lee It is a perennial rattan of the family Rosaceae. It is a perennial non-toxic, low-calorie, high-sweet and wild rare sweet plant with health care functions unique to Guangxi. It is mainly produced in Yaoshan, Jinxiu, Yongfu and Cenxi counties and cities in Guangxi. Because of its sweet taste, it is named sweet tea. Along with Mangosteen, Hepu pearls, and Guangxi spices, it is one of the four famous products in Guangxi. It has a long history of application in Guangxi folks. For a long time, the local people have been drinking it as tea, and also used it as a substitute for sugar to process food, and it can also be used as medicine. [0003] The main components of sweet tea are rubusoside, bioflavonoids, tea polyphenols, amino acids, inorganic salts, selenium, germ...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/08
CPCC07H15/256C07H1/08
Inventor 陈力健兰永凤毛丹翎
Owner 广西金秀香料香精有限责任公司
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