Mulberry silk protein extraction method

A protein extraction and mulberry silk technology, applied in the field of mulberry silk protein extraction, can solve the problems of material waste, inability to extract silk protein, incomplete extraction of silk protein, etc.

Inactive Publication Date: 2017-05-10
王玲燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction of silk protein by the above method is often not thorough, and the silk protein in the silkworm cocoon cannot be completely extracted, thereby causing a waste of mat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for extracting mulberry silk protein, the method comprising the following steps:

[0017] A. Select spring mulberry cocoons or summer and autumn mulberry cocoons, crush the cocoons into silkworm cocoon fragments with a pulverizer, put the fragments into a vacuum container with humidification and dehumidification device to balance the water, and measure the moisture regain of the fragments; weigh the fragments The dry weight of cocoons is calculated according to the moisture regain; deionized water is added to the cocoon fragments, and the silk in the fragments is fully wetted and swollen by soaking; wherein, the fineness of the crushed cocoon fragments is 400 mesh, and distilled water is added to the fragments to make them The mass percent concentration of the fragments in distilled water is 15%, and the soaking time in distilled water is 36 hours.

[0018] B, silkworm degumming bacteria are activated at least 24 hours with agar solid medium at 25 DEG C, the si...

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Abstract

The invention provides a method for preparing mulberry silk protein in the degumming process of mulberry silk protein. The method comprises the following steps: by using bombyx mori cocoons as raw materials, firstly breaking the bombyx mori cocoons into pieces by using a pulverizer, and adding deionized water to soak and carry out pretreatment; then mixing the bombyx mori cocoons with degumming bacteria to sufficiently remove gum in the bombyx mori cocoons by using the biological activity of the degumming bacteria, and finally separating the mulberry silk protein with water, and drying to obtain the mulberry silk protein.

Description

technical field [0001] The invention belongs to the field of silk processing, and in particular relates to a method for extracting mulberry silk protein. Background technique [0002] Mulberry silk is mainly composed of two proteins, sericin and silk fibroin, collectively called silk protein. Sericin surrounds the surface layer of silk fiber and acts as an adhesive. Sericin and silk fibroin have different structures and properties: sericin is a globular protein, its secondary structure is mainly random coils, and its stability is poor. It is soluble in solutions such as hot water, acid, alkali, protease and low-concentration sodium carbonate. ;Silk fibroin is a fibrous protein, containing a large number of crystal structures, stable performance, soluble in high concentration calcium chloride, calcium chloride ethanol water mixture and lithium bromide and other solutions. Therefore, according to the solubility difference of silk protein in different solutions and the actual...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/14
Inventor 王玲燕
Owner 王玲燕
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