Protoperidinuim culture medium and method
A culture medium and technology of peridinium, which is applied in the field of marine microalgae cultivation, can solve the problems of algae cells being easily polluted, and achieve the effects of promoting photosynthesis, comprehensive nutrition, and increasing yield
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0028]This embodiment provides a kind of proto-peridinium culture medium, and the formula of this proto-peridinium culture medium is as follows: NH 4 NO 3 , 30mg; CO(NH 2 ) 2 (urea), 40mg; KH 2 PO 4 , 5 mg; NaHCO 3 , 500mg; MnCl 2 4H 2 O 26g, FeC 6 h 5 o 7 ·5H 2 O (ferric citrate) 20g; VB12, 1×10 -4 mg; VB1, 100mg; KCl, 3mg; Na 2 EDTA 12.4g, chitosan oligosaccharide 1g (molecular weight 1500-2000Da), seawater 1000mL.
[0029] The preparation method of the original peridinium culture medium, comprises the steps:
[0030] 1) Sterilize the seawater for use;
[0031] 2) According to the order and dosage in the medium formula, add it into the sterilized seawater in turn, stir it fully for 5 minutes after adding it, then it can be used for inoculation.
Embodiment 2
[0033] The present embodiment provides a kind of culture method of protodinoflagellate, comprises the steps:
[0034] The first stage: Erlenmeyer flask cultivation; seawater is high-temperature sterilized seawater filtered through a 0.45μm fiber membrane, with a salinity of 30-35. After adding medium components, adjust the pH to 7.9±0.3; indoor cultivation, air-conditioned temperature control , 20±3℃; fluorescent tube lighting, light intensity 5000-7000lux, light-to-dark ratio 12h:12h;
[0035] Use a 3000-5000mL Erlenmeyer flask for culture, the working volume accounts for 3 / 5 of the volume of the flask, and inoculate the algae in the logarithmic growth phase; the inoculation density is 2×10 4 cell / mL-5×10 4 cell / mL; Shake the flask 6 times a day; algae cell density reaches 2×10 5 cell / mL-3×10 5 cell / mL, transferred to the next stage of culture;
[0036] The second stage: culture in mineral water buckets; use 18L mineral water buckets for cultivation, and use sodium hypoch...
Embodiment 3
[0041] Embodiment 3 comparative experiment
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com