Salt-tolerance denitrification microbial inoculum asynchronous domestication culture method

Abstract

The invention relates to a salt-tolerance denitrification microbial inoculum asynchronous domestication culture method. According to the method, salt-tolerance denitrification microbial inoculum is inoculated into an aerobic tank; by gradually improving the concentration of nutrient substances, after the salt-tolerance denitrification microbial inoculum gradually adapts to the ambient environment, the solid suspension concentration is increased through controlling the proper dissolved oxygen, temperature and pH; under the condition of controlling the system organic load and ammonia nitrogen load, a method of increasing the sewage concentration and reducing the nutrient substance concentration is used; according to the ammonia nitrogen removal rate of the system, the salinity load is gradually improved; the salt-tolerance denitrification microbial inoculum is domesticated. The salt-tolerance denitrification microbial inoculum obtained by the method has the advantages that the concentration is high; the activity is high; the salt-tolerance denitrification microbial inoculum per se does not have the toxic and deleterious effects on the environment; the rest silt generation quantity can be effectively reduced; the use of chemicals such as flocculating agents can be reduced. The method is applicable to salt-containing wastewater treatment industry such as chemical engineering, pharmacy, leather manufacturing, food, oil production and marine product processing; low-concentration ammonia-nitrogen wastewater can be treated; the removal effect on high-concentration ammonia nitrogen is very ideal; good application prospects are realized.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and relates to a method for asynchronous domestication and cultivation of a salt-tolerant denitrification bacterial agent. Background technique [0002] With the promulgation of the discharge standard for urban sewage treatment plants (GB18918-2002), total nitrogen was included in the discharge index for the first time. On December 15, 2011, the State Council issued the "National Environmental Protection "Twelfth Five-Year" Plan", for the first time listed ammonia nitrogen as the main binding control indicator for water pollution discharge in the country, and clearly pointed out that the total discharge of ammonia nitrogen by 2015 will be 2.38 million tons, 10% less than the same period in 2010. High-salt wastewater generally refers to wastewater with a total salt content (calculated by NaCl content) of at least 1%, which is widely found in chemical, pharmaceutical, leather, food, ...

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Problems solved by technology

However, the screening, isolation and cultivation of halophilic bacteria is a heavy workload, and it is difficult