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Method for preparing polymer microspheres by taking ferric hydroxide colloid as emulsion-method water phase

A technology for the preparation of ferric hydroxide and water phase, applied in the direction of inorganic non-active ingredients, can solve the problems of carcinogenesis and immune response, and achieve the effect of promoting proliferation and good cell compatibility

Inactive Publication Date: 2017-05-17
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, studies have shown that the residual surfactant on the microspheres will have adverse effects on the human body, such as immune response or carcinogenicity (Advanced DrugDelivery Reviews, 2008, 60, 939-954)

Method used

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  • Method for preparing polymer microspheres by taking ferric hydroxide colloid as emulsion-method water phase
  • Method for preparing polymer microspheres by taking ferric hydroxide colloid as emulsion-method water phase
  • Method for preparing polymer microspheres by taking ferric hydroxide colloid as emulsion-method water phase

Examples

Experimental program
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Effect test

Embodiment 1

[0039] (1) Weigh 0.5 g PLGA (LA / GA =85 / 15, M w =100 kDa) into 5 ml of dichloromethane, stirred at 300 rpm for 30 min to obtain an oil phase solution of PLGA;

[0040] (2) Take 60 ml FeCl with a mass fraction of 3% 3 Add the solution to 240 ml of boiling deionized water and continue heating for 2 minutes to make a ferric hydroxide colloid solution as the water phase of the emulsion;

[0041] figure 1 Fe(OH) in water phase 3 The particle size distribution diagram of colloidal nanoparticles, by figure 1 It can be seen that most Fe(OH) 3 The particle size distribution of colloidal nanoparticles is between 0 and 200nm, with an average particle size of 38.05nm;

[0042] (3) Add the oil phase solution of PLGA to the colloidal solution of ferric hydroxide dropwise under the stirring condition of 300 rpm to obtain an oil-in-water single emulsion;

[0043](4) Continuously stirred the single emulsion for 5 h, volatilized and removed the organic solvent, and obtained PLGA microspher...

Embodiment 2

[0048] (1) Weigh 0.5 g PLGA (LA / GA =85 / 15, M w =100 kDa) into 5 ml of dichloromethane, stirred at 300 rpm for 30 min to obtain PLGA oil phase solution;

[0049] (2) Weigh 1.50 g of PVA and add it to 250 ml of deionized water at 90°C, continue heating for 60 minutes to fully dissolve the PVA, and obtain an aqueous solution of PVA after cooling;

[0050] (3) Add the oil phase solution of PLGA to the PVA water phase solution dropwise under the stirring condition of 300 rpm to obtain an oil-in-water single emulsion;

[0051] (4) Stir the single emulsion continuously for 5 h, volatilize and remove the organic solvent, and obtain PLGA microspheres without iron on the surface; collect the solidified PLGA microspheres, wash with deionized water for 3 times, freeze-dry, and mark as PVA-PLGA Microspheres.

[0052] Figure 5 The overall morphology of the prepared PVA-PLGA microspheres is shown by Figure 5 It can be seen that the surface of the PVA-PLGA microspheres prepared by the t...

Embodiment 3

[0055] (1) Weigh 0.5 g PLLA (M w =50 kDa) into 5 ml of dichloromethane, stirred at 300 rpm for 30 min to obtain an oil phase solution of PLLA;

[0056] (2) Take 60 ml FeCl with a mass fraction of 3% 3 Add the solution to 240 ml of boiling deionized water and continue heating for 2 minutes to make a ferric hydroxide colloid solution as the water phase of the emulsion;

[0057] (3) Add the oil phase solution of PLLA to the colloidal solution of ferric hydroxide dropwise under the stirring condition of 300 rpm to obtain an oil-in-water single emulsion;

[0058] (4) Stir the single emulsion continuously for 5 h, volatilize and remove the organic solvent, and obtain PLLA microspheres with iron elements on the surface; collect the solidified PLLA microspheres, wash with deionized water for 3 times, freeze-dry, and mark as Fe(OH) 3 - PLLA microspheres.

[0059] Figure 7 For the preparation of Fe(OH) 3 - Overall topography of PLLA microspheres, Figure 7 Shows successful prepar...

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Abstract

The invention discloses a method for preparing polymer microspheres by taking ferric hydroxide colloid as an emulsion-method water phase. The method comprises the following steps: (1) adding polymer materials into an organic solvent, and stirring, so as to obtain a polymer oil phase solution; (2) adding a FeCl3 solution into boiling deionized water, continuously heating, and carrying out hydrolysis reaction so as to obtain a ferric hydroxide colloid solution; (3) dispersing the polymer oil phase solution into the ferric hydroxide colloid solution, so as to obtain an oil-in-water single emulsion; and (4) continuously stirring the oil-in-water single emulsion to volatilize to remove the organic solvent in the polymer oil phase solution, so as to obtain solidified polymer microspheres, washing with deionized water, and drying, so as to obtain the polymer microspheres. The surfaces of the polymer microspheres prepared by virtue of the method contain iron element, and the polymer microspheres have good cytocompatibility; and compared with polymer microspheres prepared by virtue of a traditional PVA emulsion method, the polymer microspheres prepared by virtue of the method are capable of promoting the proliferation of mesenchymal stem cells of mice.

Description

technical field [0001] The invention relates to a method for preparing polymer microspheres, in particular to a method for preparing polymer microspheres by using ferric hydroxide colloid as the aqueous phase of an emulsion method. Background technique [0002] In the field of biomedical materials, biodegradable polymer microspheres have the functions of targeted drug delivery, sustained drug release, and mimicking extracellular matrix to stimulate cellular responses. The emulsion method is the most commonly used method to prepare polymer microspheres. The traditional emulsion method to prepare biomedical microspheres always needs to add a certain amount of surfactant to the external water phase to stabilize the oil phase emulsion droplets, such as polyvinyl alcohol (Biomaterials , 2002, 23, 1649-1656), methylcellulose (Journal of Biomedical Materials Research, 1992, 26, 467-479), etc. However, studies have shown that the surfactant remaining on the microspheres will have a...

Claims

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Application Information

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IPC IPC(8): A61K47/34A61K47/02
CPCA61K47/34A61K47/02
Inventor 曹晓东张奋侯杰
Owner SOUTH CHINA UNIV OF TECH
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