Microbial-chemical composite oil displacement agent and preparation method and application thereof
A composite oil displacement agent and composite oil displacement technology, applied in the direction of chemical instruments and methods, drilling compositions, etc., can solve problems such as damage to formations, unsatisfactory oil recovery effects, and environmental pollution. Enhanced oil recovery and high recovery
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[0059] One, the preparation of Pseudomonas aeruginosa ATCC9027 fermentation broth and the content determination of rhamnolipid, the steps are as follows:
[0060] (1) Preparation of fermentation broth.
[0061] a. Prepare fermentation medium.
[0062] Weigh 30g of glycerol, NaNO 3 4g, K 2 HPO 4 1g, KH 2 PO 4 4g, NaCl 3g, MgSO 4 0.4g; K 2 SO 4 0.02g, 1000mL of purified water in a beaker, heating and stirring constantly, adjust the pH of the solution to 7.2 with 8% NaOH solution. Stop heating after the culture solution boils, cool to 60°C, divide into 5 parts and pour into the Erlenmeyer flask. Then the flask was transferred to an autoclave, and sterilized at 121° C. and 0.105 MPa for 28 minutes.
[0063] b. Vaccination. After the sterilization is completed, transfer the triangular flask to the ultra-clean workbench, put the sterilized inoculation loop into the strain tube and cool it to 30°C, then pick a loop and put it into the triangular flask immediately, and ...
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