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Bovine mycoplasma Taqman fluorescence PCR kit and using method thereof

A technology of mycoplasma bovis and a kit, applied in the field of animal medicine, to achieve the effects of fast detection, low cost and high specificity

Inactive Publication Date: 2017-05-24
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The probe is labeled with a reporter fluorescent group at the 5' end and a quencher group at the 3' end. When the probe is intact, the fluorescence emitted by the reporter fluorescent group is absorbed by the quencher group, so no fluorescence occurs

Method used

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  • Bovine mycoplasma Taqman fluorescence PCR kit and using method thereof
  • Bovine mycoplasma Taqman fluorescence PCR kit and using method thereof
  • Bovine mycoplasma Taqman fluorescence PCR kit and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0058] Embodiment detects the specificity of the real-time fluorescent quantitative detection method of Mycoplasma bovis

[0059] Mycoplasma ovis pneumoniae, Mycoplasma goat subspecies, Salmonella, Pasteurella, and Listeria were all from the Animal Disease Research Institute of Guizhou University as a template, and 10 4 The copy / μL Mycoplasma bovis cloning plasmid standard product is used as a positive control, and sterile deionized water is used as a negative control to test the specificity of the real-time fluorescent quantitative detection method for Mycoplasma bovis of the present invention. The reaction system and reaction conditions of the real-time fluorescence quantitative detection are the same as those in the examples.

[0060] The specificity detection result is as shown in the figure (curve is the positive control amplification curve, curve one is the differential strain amplification curve, and the curve is the negative control). Amplification curves (positive) o...

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PUM

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Abstract

The invention discloses a bovine mycoplasma Taqman fluorescence PCR kit and a using method thereof. The kit comprises a TaqMan probe, a PCR detection primer or a derived sequence of the primer, PCR amplification reagent, positive control and negative control. Each reagent is added according to the using method to form a reaction system, and is used for detecting a sample according to the fluorescence PCR instrument reaction condition set according to instruction of the using method, and can be used for quickly detecting the bovine mycoplasma nucleic acid in a bovine mycoplasma culture or tissue sample. The kit can be used for quickly detecting a bovine mycoplasma pneumonia suspected case on a cattle farm, and has the characteristics of simplicity, accuracy, quickness and the like.

Description

technical field [0001] The invention belongs to the detection of bovis mycoplasma disease in the field of veterinary medicine, and particularly relates to a real-time fluorescent quantitative detection kit for detecting bovis mycoplasma pathogens, special primers, probes and their application in detecting bovis mycoplasma. Background technique [0002] Mycoplasma bovis is an important pathogenic microorganism that can cause calf pneumonia and adult bovine mastitis. In 1961, American scientist Hale first isolated Mycoplasma bovis from milk with mastitis. The research on Mycoplasma bovis is getting more and more in-depth. Mycoplasma bovis belongs to Mollusca class, Mycoplasma order, Mycoplasma genus, Mycoplasma agalactiae, Mycoplasma bovis subspecies. In addition to causing the aforementioned pneumonia and mastitis. Mycoplasma bovis has been isolated from samples of sick cattle suffering from otitis, arthritis, conjunctival keratitis, reproductive tract inflammation and inf...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/689C12Q1/686C12Q2561/113C12Q2563/107C12Q2545/114C12Q2561/101C12Q2531/113
Inventor 程振涛袁海文李涛文明周碧君王开功岳筠
Owner GUIZHOU UNIV
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