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Detection method and application of teratogenic compounds in tobacco compound fertilizer

A detection method and compound fertilizer technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of lack of research reports on technology

Inactive Publication Date: 2019-01-08
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this regard, the prior art lacks further research reports

Method used

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  • Detection method and application of teratogenic compounds in tobacco compound fertilizer
  • Detection method and application of teratogenic compounds in tobacco compound fertilizer
  • Detection method and application of teratogenic compounds in tobacco compound fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This embodiment provides a method for detecting teratogenic compounds in tobacco compound fertilizer, comprising the following steps:

[0029] S1. Extraction of teratogenic substances in tobacco compound fertilizer: adopt petroleum ether, ethyl acetate, ethanol to carry out ultrasonic extraction to compound fertilizer sample respectively, remove sample residue after extraction, add appropriate amount of anhydrous sodium sulfate in the gained filtrate, remove the teratogenic substance in the filtrate Moisture, filter, collect the filtrate to obtain the sample filtrate; the sample filtrate is concentrated by rotary evaporation, and the concentrated sample is respectively dissolved in petroleum ether, ethyl acetate, and ethanol and constant volume to obtain the sample liquid to be tested;

[0030] S2. The sample liquid to be detected is detected by gas chromatography-mass spectrometry (GC-MS);

[0031] The analysis conditions of the gas chromatography are: chromatographic ...

Embodiment 2

[0038] Embodiment 2 contains the application experiment of teratogenic compound fertilizer

[0039] (1) Test materials

[0040] Fertilizers to be tested: ①Stock fertilizer (888) Arhat N:P:K=12:12:24, the experiment code is S1; P:K=12:12:24, the experimental code is S2; ③Xingyi Pingdong Tobacco Station fertilizer N:P:K=11:15:22, the experimental code is S3; ④Puan Luohan Township Luohan Village fertilizer N: P:K=12:12:24, the experiment code is S4.

[0041] Test plants: Tobacco variety K326: from Guangdong Branch of China National Tobacco Corporation, identified hosts such as tobacco, amaranth, cucumber, zucchini, etc. are kept and provided by the Plant Virus Laboratory of South China Agricultural University.

[0042] Antiserum: Cucumber mosaic virus, tobacco mosaic virus, and tobacco leaf curl virus antiserum were provided by the Plant Virus Laboratory of South China Agricultural University.

[0043] Soil for testing: Soil of the affected field: Houcaozi Group, Nagu Village,...

Embodiment 3

[0104] Embodiment 3 does not contain the application experiment of teratogenic compound fertilizer

[0105] (1) Test materials

[0106] Fertilizers to be tested: three kinds of fertilizer samples, namely tobacco-specific compound fertilizer, potassium nitrate and potassium sulfate, were randomly selected from the fertilizers purchased in Guangdong tobacco-producing areas, and the test codes were G1, G2, and G3, respectively. The sample is detected by gas chromatography-mass spectrometry (GC-MS) of the present invention, confirming that it does not contain 2-(3-methylpyridazinyl) dimethyl malonate, 9-methoxymethylphenanthrene, 3,3 -Dimethyl-2-methine 1-propionylmethyl bicyclo[2,2,1]heptane and / or (E)(3-oxo-4-fluoro-1(3H)- (isobenzofurylidene) methyl acetate.

[0107] Test plants: tobacco variety K326.

[0108] Soil for the test: collect unpolluted red clay from the construction site (no herbicides or other pesticides have been used).

[0109] (2) Potted plant bioassay exper...

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Abstract

The invention discloses a detection method and application of a teratogenic compound in a tobacco compound fertilizer. The detection method comprises the following steps: extracting a teratogenic substance in the tobacco compound fertilizer by adopting a scientific method, detecting a sample solution obtained by extraction through gas chromatography-mass spectrometry (GC-MS), and determining whether a chemical component marked by a characteristic peak with the retention time of 33 minutes contains teratogenic components, such as 2-(3-dimethyridazine) dimethyl malonate, 9-methoxymethylphenanthrene, and 3,3-dimethyl-2-methyne 1-dimethyl propionate [2,2,1] heptanes and / or (E) (3-oxo-4-fluoro-1(3H)-isobenzofuran subunit) methyl acetate, by adopting a corresponding mass spectrum computer retrieval method according to an obtained mass spectrum. The detection method determines another key influence factor which causes growth abnormity of tobacco leaves in a tobacco field: the teratogenic compound in applied fertilizer results in the growth abnormity of the tobacco leaves; an important supplementary research achievement is supplied to quinclorac teratogenic tobaccos in the existing research achievements, and a scientific basis is supplied to comprehensive checking of teratogenesis causes of the tobacco leaves and comprehensive treatment of the teratogenesis of the tobacco leaves.

Description

technical field [0001] The invention relates to the technical field of tobacco planting, in particular to a detection method and application of teratogenic compounds in tobacco compound fertilizer. Background technique [0002] Tobacco compound fertilizer contains essential plant elements such as nitrogen, phosphorus, and potassium, and the application of compound fertilizer has a decisive impact on the yield and quality of tobacco. Because of this, there are various brands of tobacco-specific compound fertilizers on the market. But these compound fertilizers are a mixed bag, and the nutrients and fertilizer effects they contain vary greatly. More importantly, apart from the nutrients specified in the standards, other ingredients contained in compound fertilizers lack monitoring and research. [0003] There was a tobacco leaf producing area in Guangdong where quinclorac was used to control weeds in paddy fields, but the tobacco grew deformed in the second year of planting, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 陈泽鹏万树青林先丰
Owner GUANGDONG BRANCH OF CHINA TOBACCO GENERAL