Domestication cultivation method of bletilla striata tissue culture seedlings

A cultivation method and technology of the bletilla striata group, applied in the field of bletilla striata, can solve the problems of affecting the normal growth of bletilla striata, easy to accumulate, waterlogging of bletilla striata, and achieve the effect of ensuring normal growth, ensuring the growth environment, and convenient management in the later stage

Active Publication Date: 2017-05-31
XUANWEI NONGSHUO AGRI SPECIAL PROD DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the substrate used for the domestication of Bletilla striata seedlings is generally a mixture of perlite, peat soil, peat, river sand, etc. Since the growth of Bletilla striata requires a lot of water, the moisture content in the substrate should be ke

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The domestication cultivation method of a kind of Bletilla striata tissue culture seedling of the present invention comprises the following steps:

[0027] A. Matrix preparation:

[0028] A1, the pine nut shell is soaked 40min in the potassium permanganate solution of 0.3% with mass concentration, pulls out and dries;

[0029] A2. Mix humus and sheep manure evenly according to the mass ratio of 3:1, then sterilize at 120 degrees Celsius for 60 minutes, and finally add water and stir evenly so that the mass fraction of water is 60% to 70%;

[0030] A3. Evenly mix sawdust, chicken manure, bean cake, sesame oil residue and water according to the mass ratio of 8:3:1:1:5, put it in a sealed container, retting for 30 days at 30°C, take it out and knead crushed, then exposed to the sun for 3 days, and then sprayed with 0.3% potassium permanganate solution for disinfection, and finally added water and stirred evenly so that the mass fraction of water was 60% to 70%;

[0031] ...

Embodiment 2

[0038] The domestication cultivation method of a kind of Bletilla striata tissue culture seedling of the present invention comprises the following steps:

[0039] A. Matrix preparation:

[0040] A1, pine nut shell is soaked 50min in the potassium permanganate solution of 0.3% with mass concentration, pulls out and dries;

[0041] A2. Mix the humus and sheep manure evenly according to the mass ratio of 4:1, then sterilize for 75 minutes at a temperature above 140 degrees Celsius, and finally add water and stir evenly so that the mass fraction of water is 60% to 70%;

[0042] A3. Mix sawdust, chicken manure, bean cake, sesame oil residue and water according to the mass ratio of 9:4:1:1:5, put it in a sealed container, retting for 40 days at 40°C, take it out and knead crushed, then exposed to the sun for 4 days, and then sprayed with a mass concentration of 0.3% potassium permanganate solution for disinfection, and finally added water and stirred evenly so that the mass fractio...

Embodiment 3

[0050]The domestication cultivation method of a kind of Bletilla striata tissue culture seedling of the present invention comprises the following steps:

[0051] A. Matrix preparation:

[0052] A1, pine nut shell is soaked 65min in the potassium permanganate solution of 0.3% with mass concentration, pulls out and dries;

[0053] A2. Mix humus and sheep manure evenly according to the mass ratio of 5:1, then sterilize at 150 degrees Celsius for 90 minutes, and finally add water and stir evenly so that the mass fraction of water is 60% to 70%;

[0054] A3. Mix sawdust, chicken manure, bean cake, sesame oil residue and water according to the mass ratio of 10:5:1:1:5, put it in a sealed container, retting at 45°C for 45 days, take it out and knead crushed, then exposed to the sun for 5 days, and then sprayed with a mass concentration of 0.3% potassium permanganate solution for disinfection, and finally added water and stirred evenly so that the mass fraction of water was 60% to 70...

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Abstract

The invention discloses a domestication cultivation method of bletilla striata tissue culture seedlings. The domestication cultivation method comprises the following steps: A, preparing a substrate, wherein the substrate comprises mixtures of pine nut shells, humus soil and sheep manure and mixtures of sawdust, chicken manure, bean cakes and sesame oil residues; B, digging a culture pool in a greenhouse, C, successively spreading the mixtures of the pine nut shells, humus soil and sheep manure and the mixtures of the sawdust, chicken manure, bean cakes and sesame oil residues in the culture pool; D, selecting the bletilla striata tissue culture seedlings, and then cleaning, carrying out disinfection and drying; E, planting the tissue culture seedlings obtained in the step D on the substrate in the culture pool, wherein the row spacing is 2 cm to 4 cm, and the plant spacing is 1 cm to 2 cm; and F, managing: controlling the temperature in the greenhouse to be within 22 to 25 DEG C, the humidity to be within 60% to 70%, the illumination intensity to be within 2500 LX to 3000 LX, and the illumination time for 10 h to 13 h daily; regularly watering; and transplanting in fields after 3 months. The bletilla striata tissue culture seedlings are domesticated by using the method, so that good growth of the tissue culture seedlings can be ensured, the ratio for meeting requirements of field transplantation is greatly increased, and the qualified rate of the tissue culture seedlings is improved.

Description

technical field [0001] The invention relates to the technical field of bletilla striata planting, in particular to a method for domesticating and cultivating bletilla striata tissue culture seedlings. Background technique [0002] Bletilla striata is a species of Bletilla striata in the family Orchidaceae. Perennial herb, 20-50 cm high, with 4-5 leaves, the bases are nested into stems, and the scapes are drawn out from the center. Racemes with several flowers; flowers purple or light red, about 5 cm in diameter, composed of 3 sepals, 2 petals and 1 specialized lip; lip 3-lobed with longitudinal pleats; stamens and The styles are conjoined to form a zygote. There is an anther at the top of the gynocolum, and a stigma recess on the upper front. There are thick rhizomes underground, like a chicken head, rich in viscosity, containing white and gum, that is, white and mannan, which can be used for medicine. paste. [0003] The artificial cultivation of Bletilla striata genera...

Claims

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Application Information

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IPC IPC(8): A01G31/00
CPCA01G24/00A01G24/23A01G31/00
Inventor 陶春艳杨光早
Owner XUANWEI NONGSHUO AGRI SPECIAL PROD DEV CO LTD
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