Method for Propagating Succulent Plants Using Bioreactor
A bioreactor, succulent plant technology, applied in the fields of botany equipment and methods, plant regeneration, gardening methods, etc., can solve the problems of easy vitrification growth rate of succulent plant materials, inability to establish a tissue culture system, and no technical advantages yet formed. , to achieve the effect of improving the normal bud yield, improving the slow growth rate and reducing labor costs
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Embodiment 1
[0033] The method for utilizing a bioreactor to propagate succulents comprises the steps of:
[0034] (1) Explant sterilization and induction
[0035] Put the mature seeds in a sterile Erlenmeyer flask, soak them in 75% ethanol for 30 seconds, rinse them with sterile water for 3-5 times, then soak them with 15% sodium hypochlorite solution for 20 minutes, and rinse them with sterile water for 3-5 times. The sterilized seeds were inoculated into induction medium.
[0036] The induction medium can be a conventional medium. In this example, the composition of the induction medium is: MS medium + BA0.5~2mg L -1 + agar 7g·L -1 + sucrose 30g·L -1 , PH value is 5.8. Wherein, BA is 6-benzylaminopurine.
[0037] After the sterilized seeds were cultured in the induction medium for 3 months, they gradually grew into complete plants, and the leaves of the plants were inoculated in the subculture medium, and after 1-3 months, embryogenic callus was induced. Most of the embryogenic ca...
Embodiment 2
[0054] Different from Example 1, in this embodiment, the leaves collected from the mother plant are used as explants, and the leaves are pretreated before sterilization. The specific method is as follows: put the leaves collected from the mother plant In a clean, ventilated, and dry environment, after 7 days, after the petiole wound has formed a protective layer, at noon on consecutive sunny days, soak the leaves in soapy water for 20 minutes, then rinse them with running water for 1 hour, and then sterilize them in an ultra-clean workbench. Bacteria, the sterilization method is the same as that of seeds. Finally, the sterilized leaves were inoculated in the induction medium, and after 1 to 3 months, embryogenic callus was formed at the wound of the leaves.
[0055] Before the leaves are sterilized, pretreatment is carried out to form a protective layer on the wounds of the petioles to prevent the leaves from rotting due to high water content. The leaves are used as induction...
Embodiment 3
[0057] The difference from Example 1 and Example 2 is that in this example, the induced embryogenic callus is propagated and cultured in the bioreactor for 3 to 4 cycles. The embryogenic material cultivated for 3 to 4 cycles is colorless and translucent, and the branches and leaves are swollen into lumps.
[0058] In this example, the time of proliferation culture was extended to 3~4 cycles, and the embryogenic callus achieved a large number of proliferation in the bioreactor, and due to the prolongation of the culture time, the embryogenic material was colorless and bright, and the branches and leaves swelled into adult cells. Lumpy.
[0059] The colorless and translucent embryogenic material that has been cultured for 3 to 4 cycles is operated in the following steps:
[0060] (3) drying and germination
[0061] Place the obtained colorless and translucent embryogenic material under sterile conditions, dehydrate quickly with dry, sterile air flow for 4-8 hours, then place u...
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