Prussian blue analog nanoparticles, and preparation method and application thereof

A Prussian blue-like and nanoparticle technology, which is applied in the field of Prussian blue-like nanoparticles and their preparation, can solve the problems that drugs cannot produce efficacy, cannot reach the interior of cells, radiotherapy fails, etc., and achieves uniform and stable preparation process and good clinical application prospects. , the effect of accelerating cell growth

Active Publication Date: 2017-05-31
SHANGHAI LINKCHEM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional surgery can be used to remove early benign and non-spread tumors, but it may not be able to completely remove the tumor, and the tumor may recur; radiotherapy uses high-energy rays to kill the tumor to achieve the purpose of treating the tumor, and early treatment can kill the tumor. A large number of tumor cells, but a small number of mutated tumor cells can continue to survive and proliferate, eventually leading to the failure of radiotherapy; chemotherapy is to kill or inhibit the growth and proliferation of tumor cells with chemical

Method used

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  • Prussian blue analog nanoparticles, and preparation method and application thereof
  • Prussian blue analog nanoparticles, and preparation method and application thereof
  • Prussian blue analog nanoparticles, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of Prussian blue-like nanoparticles with a molar ratio of iron and zinc of 3:1:

[0033] S1. Mix potassium ferricyanide, namely potassium hexacyanoferrate (0.2~0.4mol / L) and biocompatible polymer polyvinylpyrrolidone / hyaluronic acid mixed aqueous solution (0.05~0.2mol / L) evenly to obtain a mixed Liquid A; wherein, the molar ratio of polyvinylpyrrolidone / hyaluronic acid mixture to potassium hexacyanoferrate is 1: (30-50);

[0034] S2. Divalent Fe(II) and Zn(II) mixed salt (molar ratio is 3:1, concentration is 0.1~0.2mol / L) and polyvinylpyrrolidone / hyaluronic acid aqueous solution (0.05~0.2mol / L ) were mixed uniformly to obtain mixed solution B; wherein, the molar ratio of hyaluronic acid / polyvinylpyrrolidone mixture to Fe(II) and Zn(II) mixed salt was 1: (30-50); divalent Fe(II) and Zn (II) salt is preferred with sulfate and hydrochloride;

[0035] S3. Slowly add the mixed solution B to the mixed solution A, and heat to obtain an aqueous solution containing...

Embodiment 2

[0047] Preparation of Prussian blue-like nanoparticles with a molar ratio of iron and zinc of 2:1:

[0048] S1. Potassium ferricyanide, potassium hexacyanoferrate (0.2~0.4mol / L) and organic polymer surface protection agent polyvinylpyrrolidone / hyaluronic acid mixed aqueous solution (0.05~0.2mol / L) are mixed uniformly to obtain Mixture A; wherein, the molar ratio of polyvinylpyrrolidone / hyaluronic acid mixture to potassium hexacyanoferrate is 1: (30-50);

[0049] S2, mix divalent Fe(II) and Zn(II) mixed salt (molar ratio is 2:1, concentration is 0.1~0.2mol / L respectively) and polyvinylpyrrolidone / hyaluronic acid aqueous solution (0.05~0.2mol / L ) were mixed uniformly to obtain mixed solution B; wherein, the molar ratio of hyaluronic acid / polyvinylpyrrolidone mixture to Fe(II) and Zn(II) mixed salt was 1: (30-50); divalent Fe(II) and Zn (II) salt is preferred with sulfate and hydrochloride;

[0050] S3. Slowly add the mixed solution B to the mixed solution A, and heat to obtain...

Embodiment 3

[0055] Preparation of iron-zinc molar ratio of 1:1 targeted Prussian blue nanoparticles:

[0056] S1. Potassium ferricyanide, potassium hexacyanoferrate (0.2~0.4mol / L) and organic polymer surface protection agent polyvinylpyrrolidone / hyaluronic acid mixed aqueous solution (0.05~0.2mol / L) are mixed uniformly to obtain Mixture A; wherein, the molar ratio of polyvinylpyrrolidone / hyaluronic acid mixture to potassium hexacyanoferrate is 1: (30-50);

[0057] S2, the Fe 2+ / Zn 2+ Mixed salt (molar ratio 1:1, concentrations 0.1-0.2 mol / L) and polyvinylpyrrolidone / hyaluronic acid aqueous solution (0.05-0.2 mol / L) were uniformly mixed to obtain mixed solution B; among them, hyaluronic acid The molar ratio of polyvinylpyrrolidone mixture to Fe(II) and Zn(II) mixed salt is 1: (30-50); Fe 2+ / Zn 2+ Salt is preferred with sulfate and hydrochloride;

[0058] S3. Slowly add the mixed solution B to the mixed solution A, and heat to obtain an aqueous solution containing Prussian blue nanop...

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Abstract

The invention discloses Prussian blue analog nanoparticles. The nanoparticles are formed by using Prussian blue analog nanoparticles as the core and coating organic high-polymer surface protectants polyvinylpyrrolidone/chitosan and hyaluronic acid on the nanoparticle surface. The preparation method of the Prussian blue analog nanoparticles comprises the following steps: S1. preparing a mixed solution containing a zinc salt, ferrous sulfate, an iron salt and an organic high-polymer surface protectant; S2. stirring at room temperature for 30 minutes to 2 hours, putting the solution into a dialysis bag filled with distilled water, and dialyzing for two days; and S3. taking out the solid matter, and collecting the solid product by freeze-drying. The Prussian blue analog nanoparticles are used in cancer targeted therapy. The Zn-FePB NPH drug is very safe. The copper element in cancer cells can be replaced to achieve the goal of starving the cancer cells. PVP (or chitosan) doping is carried out on the basis of the high targeting action of the hyaluronic acid to better regulate the size of the nanoparticles.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a Prussian blue-like nanoparticle and a preparation method and application thereof. Background technique [0002] Cancer is a general term for various malignant tumors and a major disease that threatens human health. Cancer is generally difficult to cure. Not only the patient has a lot of mental stress, but also the family has brought great mental and material pressure. Traditional treatments include surgery, radiation and chemotherapy. Traditional surgery can be used to remove early benign and non-spread tumors, but it may not be able to completely remove the tumor, and the tumor may recur; radiotherapy uses high-energy rays to kill the tumor to achieve the purpose of treating the tumor, and early treatment can kill the tumor. A large number of tumor cells, but a small number of mutated tumor cells can continue to survive and proliferate, eventually le...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K33/30A61K33/26A61K47/36A61K47/32A61P35/00
CPCA61K9/5138A61K9/5161A61K33/26A61K33/30A61K2300/00
Inventor 陆茜
Owner SHANGHAI LINKCHEM TECH CO LTD
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