Method for treating domestic wastewater with silicon aluminum ceramic porous material immobilized microorganism
A technology for immobilizing microorganisms and porous materials, applied in biological water/sewage treatment, chemical instruments and methods, immobilized on or in inorganic carriers, etc., can solve problems such as unsatisfactory mass transfer performance, and achieve high concentration and High activity, low operating cost, enhanced treatment effect
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Embodiment 1
[0025] Step 1: Take the middle layer soil of a well-run and stable domestic sewage treatment underground infiltration system, dilute it with sterile water, shake to dissolve, let stand, and obtain bacterial suspension II;
[0026] Step 2: use the dilution plate coating method to isolate the strains, apply the second bacterial suspension on the common solid medium, observe the growth of the strains, and perform plate streaking and purification on a single colony, and obtain 11 different strains in total; The chemical composition of ordinary solid medium is: beef extract 5.0g, peptone 10.0g, sodium chloride 5.0g, agar 18g, distilled water 1L;
[0027] Step 3: Put the 11 different bacterial strains obtained in step 2 into artificially configured simulated domestic sewage with an access rate of 5%, treat the simulated domestic sewage, and draw the growth curve of the bacterial strain according to the OD600 of the bacterial liquid. When the growth rate is the fastest, the COD and N...
Embodiment 2
[0033] Step 1: Take the middle layer soil of a well-run and stable domestic sewage treatment underground infiltration system, dilute it with sterile water, shake to dissolve, let stand, and obtain bacterial suspension II;
[0034] Step 2: use the dilution plate coating method to isolate the strains, apply the second bacterial suspension on the common solid medium, observe the growth of the strains, and perform plate streaking and purification on a single colony, and obtain 11 different strains in total; The chemical composition of ordinary solid medium is: beef extract 5.0g, peptone 10.0g, sodium chloride 5.0g, agar 18g, distilled water 1L;
[0035] Step 3: Put the 11 different bacterial strains obtained in step 2 into the artificially configured simulated domestic sewage with an insertion amount of 5%, treat the simulated domestic sewage, and draw the growth curve of the bacterial strain according to the OD600 of the bacterial liquid. The growth rate is the fastest at ~48h, a...
Embodiment 3
[0041] Such as Figure 1a~1c As shown, the bacterial strain PM3 and 2g silicon-aluminum series ceramic porous material with 5% of the insertion amount are thrown into the ordinary liquid medium, and the bacterial strain PM3 adheres to and grows well; the chemical composition and concentration of the ordinary liquid medium are: beef Cream 5.0g, peptone 10.0g, sodium chloride 5.0g, distilled water 1L.
[0042] Set the culture temperature of the immobilized strain PM3 to 30°C, and at t=48h, the immobilized strain PM3 has a significant effect on COD, NH in the simulated domestic sewage 4 + The removal rates of -N and TP were 99.996%, 92.38%, 45.28%, respectively.
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