Lymphocyte culture medium

A technology of lymphocytes and culture medium, applied in the field of in vitro cell culture, can solve the problem that the quality of different batches cannot be unified, and achieve the effect of strong tumoricidal activity and strong expansion ability

Inactive Publication Date: 2017-05-31
叶宗耀
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The expansion ability and tumoricidal activity of the lymphocytes cultured in the culture medium of the present invention are enhanced, and the risk of spreading diseases existing in the existing lymphocyte culture medium and the problem that the quality of different batches cannot be unified at the same time are effectively solved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 a kind of lymphocyte culture medium

[0021] A lymphocyte culture medium, consisting of the following components and their concentrations: basal medium dry powder 10g / L, insulin 5mg / L, human transferrin 10mg / L, hydrocortisone 20mg / mL, casein phosphopeptide 25mg / L, epidermal growth factor 20ng / mL, L-glutamine 0.1g / L, ethanolamine 2mg / L, soybean polypeptide 10g / L, lecithin 1g / L, sodium selenite 0.1mg / L, iron citrate 1mg / L, zinc sulfate 1mg / L, vitamin E 1mg / L and gentamicin 1IU / mL.

[0022] The preparation method is divided into the following steps:

[0023] S1 Accurately weigh the dry powder of the basic medium, casein phosphopeptide, L-glutamine, ethanolamine, sodium selenite, ferric citrate and zinc sulfate, dissolve them in ultrapure water at 40-45°C, stir well, Obtain solution I;

[0024] S2 When solution I is cooled to 15-20°C, add corresponding weights of insulin, human transferrin, hydrocortisone, epidermal growth factor, lecithin, vitamin A and g...

Embodiment 2

[0026] Embodiment 2 a kind of lymphocyte culture medium

[0027] A lymphocyte culture medium, consisting of the following components and their concentrations: basal medium dry powder 20g / L, insulin 7mg / L, human transferrin 20mg / L, hydrocortisone 30mg / mL, casein phosphopeptide 35mg / L, epidermal growth factor 40ng / mL, L-glutamine 1.5g / L, ethanolamine 4mg / L, lecithin 3g / L, soybean polypeptide 20g / L, sodium selenite 1mg / L, iron citrate 5mg / L L, zinc sulfate 5mg / L, vitamin E 50mg / L and gentamicin 15IU / mL.

[0028] The preparation method is similar to Example 1.

Embodiment 3

[0029] Embodiment 3 a kind of lymphocyte culture medium

[0030] A lymphocyte culture medium, consisting of the following components and their concentrations: RPMI1640 dry powder 15g / L, insulin 6.5mg / L, human transferrin 13.75mg / L, hydrocortisone 18.25mg / mL, casein phosphopeptide 33.5mg / L, epidermal growth factor 27.5ng / mL, L-glutamine 1.32g / L, ethanolamine 3.8mg / L, soybean polypeptide 15g / L, lecithin 2.5g / L, sodium selenite 0.58mg / L , ferric citrate 4.5mg / L, zinc sulfate 3.25mg / L, vitamin E 12.5mg / L and gentamicin 7.5IU / mL.

[0031] The preparation method is similar to Example 1.

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Abstract

The invention belongs to the technical field of in-vitro cell culture, and relates to a lymphocyte culture medium. The lymphocyte culture medium is prepared from the following components: basal culture medium dry powder, insulin, human transferrin, hydrocortisone, casein phosphopeptides, an epidermal growth factor, L-glutamine, ethanol amine, soybean peptides, lecithin, sodium selenite, ferric citrate, zinc sulfate, vitamin E and gentamicin. By adopting the lymphocyte culture medium, the proliferation ability and the sterilizing activity of cultured lymphocytes are enhanced, and the problems that a disease transmission risk exists and that the quality of different batches cannot be unified in the conventional lymphocyte culture medium are solved effectively.

Description

technical field [0001] The invention belongs to the technical field of in vitro cell culture, and relates to a lymphocyte culture medium. Background technique [0002] Lymphocytes are an important carrier for cellular immunotherapy, and the in vitro culture, expansion and activation of lymphocytes are inseparable from excellent medium. The medium provides the basic environment for the growth of lymphocytes, including providing suitable osmotic pressure and pH value for the survival of lymphocytes, and providing all the nutritional factors required for the growth of lymphocytes, including inorganic salts, amino acids, functional Proteins, vitamins, etc., are also the discharge sites of various metabolites of lymphocytes. In order to provide these nutritional factors, traditional culture media mostly use animal or human serum and its components as additives. The more common ones include newborn bovine serum, fetal bovine serum, human AB serum, bovine serum albumin, and human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0781C12N5/0783
CPCC12N5/0635C12N5/0636C12N5/0646C12N2500/22C12N2500/25C12N2500/30C12N2500/32C12N2500/36C12N2500/38C12N2500/46C12N2501/11C12N2501/39C12N2501/998
Inventor 叶宗耀
Owner 叶宗耀
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