Unlock instant, AI-driven research and patent intelligence for your innovation.

Culture medium for producing flavones by cultivating eucommia bark cells and preparation method of culture medium

A technology for the production of flavonoids and culture medium by cells, which is applied in the direction of cell culture medium, biochemical equipment and methods, plant cells, etc. It can solve the problems of unstable yield and quality, slow growth of Eucommia ulmoides, and the scarcity of wild resources, so as to solve the problem of eucommia resources Shortage, clear ingredients, and the effect of protecting the ecological environment

Inactive Publication Date: 2017-05-31
安徽檀鑫科技有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its wild resources are becoming increasingly scarce. Due to the slow growth of Eucommia ulmoides, it usually takes 15 years to provide commercial products. However, the content of effective medicinal ingredients in commercial medicinal materials is extremely low, and the yield and quality are not stable.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture medium for producing flavones by cultivating eucommia bark cells and preparation method of culture medium
  • Culture medium for producing flavones by cultivating eucommia bark cells and preparation method of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, a kind of medium for cultivating eucommia cells to produce flavonoids

[0033] The medium for cultivating Eucommia ulmoides cells to produce flavonoids includes basal medium and additives added in the basal medium, and the ingredients of the additives include: glycerol 30mg / L, nicotinic acid 0.05mg / L in terms of final concentration , potassium iodide 2mg / L, aminoacetic acid 0.3mg / L, thiamine 3mg / L, activated carbon 0.02g / L, glutathione 20μg / L, luteolin 40μg / L, cytosine 4mg / L and naphthaleneacetic acid 9mg / L.

[0034] The basal medium is MS medium.

[0035] Preparation:

[0036] S1 Add niacin, glutathione, and luteolin to the basal medium, stir for 24 minutes, add potassium iodide, glycine, thiamine, activated carbon, cytosine, and naphthaleneacetic acid, continue stirring for 18 minutes, add glycerol, and stir 10 minutes to get the mixture;

[0037] S2 Adjust the pH of the mixture obtained in step S1 to 5.6, the sterilization temperature is 115° C., a...

Embodiment 2

[0038] Embodiment 2, a kind of medium for culturing Eucommia cells to produce flavonoids

[0039] The medium for cultivating Eucommia ulmoides cells to produce flavonoids includes basal medium and additives added in the basal medium, and the ingredients of the additives include: glycerol 50mg / L, nicotinic acid 0.1mg / L in terms of final concentration , potassium iodide 4mg / L, aminoacetic acid 0.8mg / L, thiamine 7mg / L, activated carbon 0.1g / L, glutathione 50μg / L, luteolin 80μg / L, cytosine 10mg / L and naphthaleneacetic acid 15mg / L.

[0040] The basal medium is White medium.

[0041] Preparation:

[0042] S1 Add niacin, glutathione, and luteolin to the basal medium, stir for 36 minutes, add potassium iodide, glycine, thiamine, activated carbon, cytosine, and naphthaleneacetic acid, continue stirring for 24 minutes, add glycerol, and stir 10 minutes to get the mixture;

[0043] S2 Adjust the pH of the mixture obtained in step S1 to 6.0, the sterilization temperature is 122° C., ...

Embodiment 3

[0044] Embodiment 3, a kind of medium for cultivating Eucommia ulmoides cells to produce flavonoids

[0045] The medium for culturing Eucommia cells to produce flavonoids includes basal medium and additives added in the basal medium, and the ingredients of the additives include: glycerol 38mg / L, nicotinic acid 0.08mg / L in terms of final concentration , potassium iodide 3mg / L, aminoacetic acid 0.6mg / L, thiamine 5mg / L, activated carbon 0.07g / L, glutathione 42μg / L, luteolin 60μg / L, cytosine 8mg / L and naphthaleneacetic acid 12mg / L.

[0046] The basal medium is White medium.

[0047] Preparation:

[0048] S1 Add niacin, glutathione, and luteolin to the basal medium, stir for 28 minutes, add potassium iodide, glycine, thiamine, activated carbon, cytosine, and naphthaleneacetic acid, continue stirring for 22 minutes, add glycerol, and stir 10 minutes to get the mixture;

[0049] S2 Adjust the pH of the mixture obtained in step S1 to 5.7, the sterilization temperature is 118° C.,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of plant cell culture media, and particularly relates to a culture medium for producing flavones by cultivating eucommia bark cells and a preparation method of the culture medium. The culture medium comprises a basal culture medium and an additive added into the basal culture medium. The component of the additive comprises, in final concentration, 30-50mg / L of glycerinum, 0.05-0.1mg / L of nicotinic acid, 2-4 mg / L of potassium iodide, 0.3-0.8mg / L of glycine, 3-7mg / L of thiamine, 0.02-0.1g / L of activated carbon, 20-50mug / L of glutathione, 40-80mug / L of luteolin, 4-10mg / L of cytosine and 9-15mg / L of naphthalene acetic acid. The culture medium is specific in component, low in cost, high in flavone content and stable in yield and quality, and daily application, wide popularization and industrialization culture are facilitated.

Description

technical field [0001] The invention belongs to the technical field of plant cell culture medium, in particular to a culture medium for cultivating eucommia cells to produce flavonoids and a preparation method thereof. Background technique [0002] Eucommia ulmoides is a perennial deciduous tree in the family Eucommiaceae. Eucommia ulmoides is a special ancient tree species in my country and belongs to the second-class national protected tree species. Eucommia ulmoides is a precious medicinal plant. Both bark and leaves can be used as medicine. It tastes sweet, slightly pungent, and warm in nature. , fatigue, dizziness, frequent urination, impotence, nocturnal emission, weak fetal element, high blood pressure, etc. The bark contains resin. Pharmacological studies have confirmed that bark extract has antihypertensive and diuretic effects, and can reduce the absorption of cholesterol. It is a good anti-aging natural medicine. Eucommia leaves also contain more than a dozen m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06C12N5/04
CPCC12P17/06C12N5/0025C12N2500/12C12N2500/30C12N2500/35
Inventor 夏国顺
Owner 安徽檀鑫科技有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com