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Method for separating and determining multiple phenolic substances in saliva

A technology for simultaneous separation of phenolic substances, applied in the field of analytical chemistry, can solve the problems of affecting the accuracy of measurement results, easy oxidation of phenolic substances, complicated processing procedures, etc., and achieves improved measurement accuracy, high recovery rate, and simple method operation. Effect

Active Publication Date: 2017-05-31
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the determination of phenolic substances by gas chromatography-mass spectrometry is easy to be oxidized or decomposed by heat, and derivatization treatment is usually required, and the operation is very cumbersome, which affects the accuracy of the determination results.
When the conventional high performance liquid chromatography is used for determination, there are few targets to be determined at the same time, the separation time is long, and the pretreatment process is cumbersome

Method used

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  • Method for separating and determining multiple phenolic substances in saliva
  • Method for separating and determining multiple phenolic substances in saliva
  • Method for separating and determining multiple phenolic substances in saliva

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for separating and measuring multiple phenolic substances in the saliva of flue-cured tobacco smokers, comprising the following steps:

[0039] (1) Sample collection: put the cotton swab in the saliva collection tube in the mouth, chew it for 2 minutes, put it back into the collection tube, and centrifuge at 10,000 rpm for 15 minutes to obtain the saliva sample;

[0040] (2) Sample acidification: pipette 0.5mL saliva sample into the sample tube, add 0.01mL acetic acid, and add 4-fluorophenol internal standard to make the concentration 3mg / L, vortex at 2000rpm for 2min until mixed evenly ;

[0041] (3) Determination: The acidified solution obtained in step (2) is passed through a microporous membrane, measured by ultra-high performance liquid chromatography-multi-wavelength fluorescence method, quantified by the internal standard method, and the content of the target substance is calculated according to the standard curve. The ultra-high performance chromatogra...

Embodiment 2

[0056] A method for separating and measuring multiple phenolic substances in the saliva of cigar smokers, comprising the following steps:

[0057] (1) Sample collection: put the cotton swab in the saliva collection tube in the mouth, chew it for 1 minute, put it back into the collection tube, and centrifuge at 12000 rpm for 12 minutes to obtain the saliva sample;

[0058] (2) Sample acidification: pipette 2.0mL saliva sample into the sample tube, add 0.03mL acetic acid, and add 4-fluorophenol internal standard to make the concentration 1.5mg / L, vortex at 20000rpm for 0.5min to well mixed;

[0059] (3) Determination: The acidified solution obtained in step (2) is passed through a microporous membrane, measured by ultra-high performance liquid chromatography-multi-wavelength fluorescence method, quantified by the internal standard method, and the content of the target substance is calculated according to the standard curve.

[0060] The ultra-high performance chromatographic co...

Embodiment 3

[0074] A method for separating and measuring multiple phenolic substances in the saliva of fragrance formulators, comprising the following steps:

[0075] (1) Sample collection: put the cotton swab in the saliva collection tube in the mouth, chew it for 3 minutes, put it back into the collection tube, and centrifuge at 15000 rpm for 10 minutes to obtain the saliva sample;

[0076] (2) Sample acidification: pipette 5.0mL saliva sample into the sample tube, add 0.025mL acetic acid, and add 4-fluorophenol internal standard to make the concentration 10mg / L, vortex at 500rpm for 10min until mixed evenly ;

[0077] (3) Determination: The acidified solution obtained in step (2) is passed through a microporous membrane, measured by ultra-high performance liquid chromatography-multi-wavelength fluorescence method, quantified by the internal standard method, and the content of the target substance is calculated according to the standard curve.

[0078] The ultra-high performance chroma...

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Abstract

The invention relates to a method for separating and determining multiple phenolic substances in saliva, and belongs to the technical field of analytical chemistry. The method comprises the steps of collecting a saliva sample by adopting a saliva collection tube, carrying out high-speed centrifugation after collection and then taking a supernatant; carrying out acidification, adding an internal standard and carrying out vortex oscillation until mixing evenly in sequence; and filtering with a micropore filter membrane, separating and determining multiple phenolic substances in saliva from filtrate by adopting an ultra-high performance liquid chromatography-multiwavelength fluorescence method, fabricating a standard curve and quantifying through an internal standard method. The method can be effectively used for separating and determining the multiple phenolic substances in the saliva, and the method is good in separation effect, low in detection limit, good in precision, accurate and reliable, and has popularization and application values.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, in particular to a method for simultaneously separating and measuring multiple phenolic substances in saliva. Background technique [0002] Phenol, hydroquinone, methyl phenol, etc., not only have a bad smell, but also have a strong stimulating effect and carcinogenic effect on the skin and respiratory mucosa. Other phenols, such as eugenol and 2-methoxy-4-methyl-phenol, have a strong aroma and are one of the important ingredients in essence. These phenolic substances exist in large quantities in the mainstream smoke of cigarettes, and can be dissolved and absorbed through the saliva of tobacco smokers and passive smokers, and then have an impact on the human body. In addition, phenols may exist in the saliva of people exposed to phenols in large quantities, and phenols may also exist in the saliva of phenol poisoning caused by inhalation of phenols in large quantities. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 司晓喜刘志华张凤梅朱瑞芝朱琴杨光宇何沛刘春波申钦鹏尤俊衡王昆淼王晋
Owner CHINA TOBACCO YUNNAN IND
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