Reducing response magnetic drug-loaded nanoparticles with synergetic anti-cancer interaction and preparation method of reducing response magnetic drug-loaded nanoparticles

A drug-loaded nanometer and inorganic nanoparticle technology, applied in the field of medicine, can solve the problems of reduced drug availability, normal tissue damage, and large dosage, and achieve strong reduction-sensitive release, low toxic and side effects, and improved inhibition. Effect

Inactive Publication Date: 2017-06-13
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the weak interaction between the carrier and the drug, the drug is usually released early or violently in the systemic circulation, which reduces the availability of the drug and causes damage to normal tissues, resulting in greater toxic and side effects
In addition, the single-drug carrier has the disadvantages of large dosage and easy to cause drug resistance.

Method used

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  • Reducing response magnetic drug-loaded nanoparticles with synergetic anti-cancer interaction and preparation method of reducing response magnetic drug-loaded nanoparticles
  • Reducing response magnetic drug-loaded nanoparticles with synergetic anti-cancer interaction and preparation method of reducing response magnetic drug-loaded nanoparticles
  • Reducing response magnetic drug-loaded nanoparticles with synergetic anti-cancer interaction and preparation method of reducing response magnetic drug-loaded nanoparticles

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Experimental program
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Effect test

Embodiment 1

[0028] Preparation of magnetic selenium-containing disulfide bonded drug-loaded nanoparticles, comprising the following steps:

[0029] 1. Preparation of chitosan template nano-selenium (CS-SeNPs) stock solution:

[0030] 24 mg chitosan was dissolved in 10 mL acetic acid (w / v, 1%) to obtain a chitosan (CS) solution with a concentration of 2.4 mg / mL. Weigh 1.4g (8mmol) of ascorbic acid (VC) and dissolve it in 10mL of ultrapure water to obtain a VC solution. Weighing 346mg (2mmol) sodium selenite was dissolved in 10mL ultrapure water to obtain a sodium selenite solution with a concentration of 200mmol / L. Then put the prepared CS solution and sodium selenite solution into the beaker, and after mixing evenly by magnetic stirring, slowly add the VC solution dropwise, seal it with a plastic wrap and continue stirring. The color of the solution gradually changed from clear light yellow to thick dark red. After stirring for 12 h, the crude product was obtained. Then put it into a ...

Embodiment 2

[0046] 1. Preparation of polylysine template nano-selenium (PAM-Se NPs) dispersion:

[0047] First, 20 mg of polylysine was dissolved in 10 mL of deionized water to obtain a polylysine solution with a concentration of 2.0 mg / mL. Weigh 1.4 g of ascorbic acid (VC) and dissolve it in 10 mL of ultrapure water to obtain a VC solution. Weigh 346 mg of sodium selenite and dissolve it in 10 mL of ultrapure water to obtain a sodium selenite solution. Put the prepared polylysine solution and sodium selenite into a beaker, stir and mix evenly, slowly add the VC solution dropwise, seal it with a plastic wrap and continue stirring. The color of the solution gradually changed from clear light yellow to thick dark red. After stirring for 12 h, the crude product was obtained. Then put it into a dialysis bag (MWCO 14000) for dialysis in ultrapure water, put it in a high-speed centrifuge, and centrifuge it at room temperature at 1500rpm for 10 minutes, take out the supernatant and continue t...

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Abstract

The invention relates to reducing response magnetic drug-loaded nanoparticles with synergetic anti-cancer interaction and a preparation method of the reducing response magnetic drug-loaded nanoparticles. The drug-loaded nanoparticles are prepared from a polymer prodrug and inorganic nanoparticles, which are connected through a disulfide bond; and the inorganic nanoparticles include surface-aminated superparamagnetism ferroferric oxide nanoparticles (SPION-NH2) and aminated selenium nanoparticles (NH2-R-SeNPs) employing an amino-containing high polymer as a template. The preparation method specifically comprises the following steps: (1) mixing an SPION-NH2 solution with an NH2-R-SeNPs dispersing liquid and adjusting the pH value to be 4.5-5.5; (2) dissolving the polymer prodrug connected through the disulfide bond by using ultrapure water and adjusting the pH value to be 5; and (3) dropwise adding the solution obtained in step (1) to the solution obtained in step (2), adjusting the pH value of a system to be 7.4, stirring the solution at room temperature for 10-14h to obtain the reducing response magnetic drug-loaded nanoparticles with synergetic anti-cancer interaction. The drug-loaded nanoparticles have the advantages of specific responsiveness in a targeted area, synergetic interaction and a low toxic or side effect.

Description

technical field [0001] The invention relates to a reduction-responsive magnetic drug-loaded nanoparticle with synergistic anticancer and synergistic effects and a preparation method thereof, belonging to the technical field of medicine. Background technique [0002] In traditional anti-cancer chemotherapy, small molecule drugs often have high toxicity to normal tissues, are easily cleared by the reticuloendothelial system (RES), and cannot avoid the low drug utilization caused by the shielding effect in cells , adverse effects such as large toxic and side effects; and the defects of low water solubility, poor stability and easy drug resistance of small molecule drugs have become an insurmountable gap in chemical drug treatment. In anti-cancer research in recent years, in response to the above problems, nanoparticles, as a new type of carrier, have attracted extensive attention from the medical community. Well-encapsulated and selectively enriched in the lesion, it effective...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/06A61K47/61A61K47/59A61K47/52A61K33/04A61P35/00A61K31/704
CPCA61K45/06A61K31/704A61K33/04A61K2300/00
Inventor 喻发全喻波陈黎迪薛亚楠龙思会
Owner WUHAN INSTITUTE OF TECHNOLOGY
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