A kind of degradable polymer magnetic nanoparticles and preparation method thereof

Abstract

The invention discloses a degradable polymer magnetic nanoparticle and a preparation method thereof. The preparation method utilizes a divinyl disulfide bond-containing compound as a coupling agent and comprises that dopamine, an antitumor drug group and a targeting functional group are connected to the main chain of the coupling agent through covalent bonds so that a degradable shell is prepared through polymerization, and the degradable shell chemically coats outer surfaces of ferroferric oxide nanoparticles so that dopamine on the degradable shell can be bonded to the surfaces of the ferroferric oxide nanoparticles through coordination bonds and the degradable polymer magnetic nanoparticle is formed. The polymer shell is degradable and can be stably bonded to the magnetic nanoparticles through coordination bonds. The degradable polymer magnetic nanoparticle has good drug loading stability and has a targeting function and good contrast effects.

Description

technical field [0001] The invention belongs to the technical field of degradable polymer magnetic nanoparticles and its preparation, and in particular relates to a method of wrapping a degradable polymer material in a magnetic nanoparticle shell as a drug carrier and a preparation method thereof, in particular to a method for wrapping a degradable polymer material in a magnetic nanoparticle shell. Degradable polymer material as drug carrier with targeting function and preparation method thereof. Background technique [0002] In recent years, based on Fe 3 o 4 The magnetic nanoparticles have the advantages of superparamagnetism and low cytotoxicity, and have been widely used in the integrated research of drug delivery (therapy) and magnetic resonance imaging (medical diagnosis). In order to improve the stability of magnetic nanoparticles, a technical solution generally adopted by those skilled in the art is to prepare polymer magnetic nanoparticles by wrapping a layer of p...

AI Technical Summary

Problems solved by technology

However, with the in-depth research on polymer magnetic nanoparticles, it is found that there are still some defects that restrict the large-scale clinical application of magnetic nanoparticles in the process of realizing diagnosis and treatment:

[0003] (1) The polymer shell cannot be degraded

The technology of using polymer materials as shells to wrap magnetic nanoparticles to form polymer magnetic nanoparticles has the advantages of simple method and clear nanoparticle structure, but the formed polymer shells generally have no degradation function and are easy to occur in the human body. Aggregation, thus causing toxicity to liver, kidney and other organs (Fang C., Kievit F.M., Veiseh O., Stephen Z.R., Wang T., Lee D., Ellenbogen R.G., Zhang M. Fabrication of magnetic nanoparticles with controllable drug loading and release through a simple assembly approach. Journal of Controlled Release. 2012,162:233–241)

[0004] (2) Insufficient stability of nanoparticles

Due to the weak interaction between the drug and the polymer formed by this technical means, the loading stability of the drug is also poor, and more drugs are often released in advance in normal tissues and cells. Particles generally have toxic and side effects on normal tissue cells (Chen J., Shi M., Liu P., Ko A., Zhong W., Liao W., Xing M., M.Q. Reducible polyamidoamine-magnetic iron oxide self-assembled nanoparticles fordoxorubicin delivery. Biomaterials, 2014, 35:1240–1248)

[0005] (3) No targeting function, so that the polymer magnetic nanoparticles cannot be released accurately after drug loading

Although this technology has the advantage of simple preparation method, the polymer magnetic nanoparticles prepared by it have no targeting function, and the polymer magnetic nanoparticles cannot be effectively delivered into the targeted cells, and released to kill tumor cells.

Therefore, drug-loaded polymer magnetic nanoparticles are prone to aggregation in normal tissues and cells, and there is a risk of side effects (Shang Le, Zhou Qinghan. Preparation and Characterization of Multifunctional Magnetic Nano-Drug Carriers. Journal of Southwest University for Nationalities (Natural Science Edition) ),2016,42(5):531-537)

[0006] (4) Poor imaging effect

The polymer magnetic nanoparticles prepared by traditional technology, because the interaction force between the polymer material and the magnetic nanoparticles is weak (such as non-covalent bond forces such as hydrophobic bonds and hydrogen bonds), the content of the magnetic nanoparticles wrapped is relatively small , leading to the transverse relaxation properties (r 2 ) is low, and the contrast effect is not good, which affects the clinical application of polymer magnetic nanoparticles as magnetic contrast (Liu Y., Yang K., Cheng L., Zhu J., Ma X., Xu H., Li Y. , Guo L., Gu H., Liu Z. PEGylated FePt@Fe 2 o 3 core-shell magnetic nanoparticles: Potential theranostic applications and in vivo toxicity studies. Nanomedicine: Nanotechnology, Biology, and Medicine, 2013, 9:1077–1088)

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