Cooperative and efficient pig manure and rice straw anaerobic digestion method
An anaerobic digestion and rice straw technology, which is applied in the field of agricultural and animal husbandry waste treatment, can solve the problems of restricting efficient resource conversion of agricultural waste, waste of fibrous resources, etc., and achieves the effect of improving fermentation efficiency and methane production capacity.
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Embodiment 1
[0053] This embodiment provides a method for synergistic anaerobic digestion of pig manure and rice straw, comprising the following steps:
[0054] S201: Inoculate Paenibacillus thermophilus NCB (preservation number: CCTCC NO: M2017076) into the first culture medium, and culture it statically at 55°C for 20 hours to obtain a strain content of (1-2)×10 6 Paenibacillusthermophilus NCB strain liquid of cfu / mL; wherein, the pH value of the first medium is 7.2, and the composition of the first medium includes: sodium chloride 5.0g / L, peptone 5.0g / L and yeast extract 1.0g / L L, the rest is water;
[0055] S202: Inoculate Clostridium clariflavum NC-1 (preservation number: CCTCC NO: M2017075) into the second culture medium, and culture it under anaerobic conditions at 55°C for 48 hours to obtain a strain content of (1-2)×10 6Clostridium clariflavum NC-1 strain liquid of cfu / mL; wherein, the pH value of the second medium is 7.2, and the composition of the second medium includes: filter...
Embodiment 2
[0063] This embodiment provides a method for synergistic anaerobic digestion of pig manure and rice straw, comprising the following steps:
[0064] S201: Inoculate Paenibacillus thermophilus NCB (preservation number: CCTCC NO: M2017076) into the first medium, and culture it statically at 50°C for 20 hours to obtain a strain content of (1-2)×10 6 Paenibacillusthermophilus NCB strain liquid of cfu / mL; wherein, the pH value of the first medium is 7.2, and the composition of the first medium includes: sodium chloride 5.0g / L, peptone 5.0g / L and yeast extract 1.0g / L L, the rest is water;
[0065] S202: Inoculate Clostridium clariflavum NC-1 (preservation number: CCTCC NO: M2017075) into the second culture medium, and culture it under anaerobic conditions at 50°C for 48 hours to obtain a strain content of (1-2)×10 6 Clostridium clariflavum NC-1 strain liquid of cfu / mL; wherein, the pH value of the second medium is 7.2, and the composition of the second medium includes: filter paper ...
Embodiment 3
[0071] This embodiment provides a method for synergistic anaerobic digestion of pig manure and rice straw, comprising the following steps:
[0072] S201: Inoculate Paenibacillus thermophilus NCB (preservation number: CCTCC NO: M2017076) into the first culture medium, and culture it statically at 52°C for 20 hours to obtain a strain content of (1-2)×10 6 Paenibacillusthermophilus NCB strain liquid of cfu / mL; wherein, the pH value of the first medium is 7.2, and the composition of the first medium includes: sodium chloride 5.0g / L, peptone 5.0g / L and yeast extract 1.0g / L L, the rest is water;
[0073] S202: Inoculate Clostridium clariflavum NC-1 (preservation number: CCTCC NO: M2017075) into the second culture medium, and culture it under anaerobic conditions at 52°C for 48 hours to obtain a strain content of (1-2)×10 6 Clostridium clariflavum NC-1 strain liquid of cfu / mL; wherein, the pH value of the second medium is 7.2, and the composition of the second medium includes: filte...
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