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Fresh cordyceps sinensis extract with antitumor activity as well as preparation method and applications thereof

A technology of fresh Cordyceps sinensis and anti-tumor activity, which is applied in the field of traditional Chinese medicine and natural medicine, and can solve the problems of early recurrence and metastasis, high degree of malignancy, and poor prognosis, and achieve the effect of clear active ingredients, exerting drug effects, and simple operation

Active Publication Date: 2017-06-20
DONGGUAN HEC CORDYCEPS R&D CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of studies have shown that 30% of breast cancer patients overexpress the HER2 gene. These patients have high tumor malignancy, early recurrence and metastasis, poor prognosis, and resistance to certain chemotherapy drugs.

Method used

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  • Fresh cordyceps sinensis extract with antitumor activity as well as preparation method and applications thereof
  • Fresh cordyceps sinensis extract with antitumor activity as well as preparation method and applications thereof
  • Fresh cordyceps sinensis extract with antitumor activity as well as preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Take 10g of fresh Cordyceps sinensis, cut it into pieces, add 80mL of water, homogenate for 4 minutes, the homogenization speed is 14000rpm, and the temperature of homogenization is controlled at 15±2°C to obtain a suspension of fine particles of fresh Cordyceps sinensis. Ultrasonic extraction of the obtained homogenized Cordyceps sinensis was carried out for 20 minutes, the ultrasonic temperature was controlled at 15±2°C, the power was 400W, and the supernatant was obtained by centrifugation at 8000rmp for 10 minutes, and then the fresh residue of Cordyceps sinensis was frozen at -20°C Set for 20min. Homogenize again for 4 minutes at a homogenization speed of 14,000 rpm, and disperse the frozen fresh Cordyceps residue evenly, repeat ultrasonic extraction for 20 minutes, control the temperature at 15±2°C, and power 400W. The suspension after repeated ultrasonication was centrifuged for 10 min at a rotation speed of 8000 rpm, and the supernatants were combined twice. Ad...

Embodiment 2

[0049] Take 10g of fresh Cordyceps, cut them into pieces, add 100mL of water, homogenate for 6 minutes, the homogenization speed is 16000rpm, and the temperature of homogenization is controlled at 20±2°C, and the fresh Cordyceps is crushed into a suspension of fine particles. The homogenized fresh Cordyceps sinensis was subjected to ultrasonic extraction for 30 minutes, the ultrasonic temperature was controlled at 20±2°C, the power was 500W, and the supernatant was obtained by centrifugation at 9000rpm for 12 minutes, and then the fresh Cordyceps suspension was placed at -20°C Stand in the freezer for 40 minutes. Homogenize again for 6 minutes at a homogenization speed of 16,000 rpm, and disperse the frozen fresh Cordyceps suspension evenly, repeat ultrasonic extraction for 30 minutes, control the ultrasonic temperature to 20±2°C, and the power to 500W. The suspension after repeated ultrasonication was centrifuged for 12 min at a rotation speed of 9000 rpm, and the supernatant...

Embodiment 3

[0051] Take 10g of fresh Cordyceps sinensis, cut it into pieces, add 120mL of water, homogenate for 8min, rotate at 18000rpm, control the homogenization temperature at 25±2°C, and then obtain fresh Cordyceps sinensis and crush it into a suspension of fine particles. Ultrasonic extraction of the fresh fine particle suspension of Cordyceps sinensis was carried out for 40 minutes, the ultrasonic temperature was controlled at 25±2°C, the power was 600W, and the supernatant was obtained by centrifugation at 10,000 rpm for 15 minutes, and then the suspension of Cordyceps sinensis was placed at -20°C Freeze for 60 minutes. Homogenize and disperse the evenly frozen Cordyceps suspension again, repeat the ultrasonic extraction for 40min, the temperature is controlled at 25±2°C, and the power is 600W. The suspension after repeated ultrasonication was centrifuged for 15 min at a rotation speed of 10000 rpm, and the supernatants were combined twice. Add absolute ethanol to the supernatant...

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Abstract

The invention discloses fresh cordyceps sinensis extract with the antitumor activity as well as a preparation method and applications of the fresh cordyceps sinensis extract in the field of the medicines for resisting breast cancer and resisting melanoma. The preparation method of the fresh cordyceps sinensis extract comprises the following steps: grinding fresh cordyceps sinensis, adding with water, carrying out homogenizing, carrying out low-temperature ultrasonic auxiliary extraction on cordyceps sinensis, carrying out centrifugalizing, taking the supernatant, carrying out concentrating, carrying out alcohol precipitation, collecting the sediment, and carrying out freeze drying, thus obtaining the cordyceps sinensis extract. The obtained fresh cordyceps sinensis extract has the obvious effects of inhibiting breast cancer cell proliferation and resisting melanoma metastasis, meanwhile, the active ingredients are definite, the fresh cordyceps sinensis extract can be used for preventing and treating cancer, and thus the fresh cordyceps sinensis extract has the good medicinal prospect; during the preparation process, the poisonous and harmful organic solvents are not introduced, so that the use is safe; the preparation process is simple, and the large-scale production can be easily realized.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine and natural medicine, in particular to a fresh Cordyceps sinensis extract with anti-tumor activity, its preparation method and its application in the fields of anti-breast cancer and anti-melanoma metastasis drugs. Background technique [0002] Cordyceps sinensis is a composite of the subsealet and larvae corpse of Cordyceps sinensis (Berk.) Sacc., a fungus of the family Ergotaceae. and other effects. Modern medical research shows that Cordyceps sinensis has the functions of anti-tumor, regulating immunity, lowering blood pressure, regulating endocrine, calming, anti-myocardial ischemia and regulating heart rate. In recent years, a large number of literatures have reported that Cordyceps sinensis and its active ingredients can inhibit the growth and metastasis of gastric cancer, lung cancer, thyroid cancer and other tumor cells, but there are relatively few studies on breast cancer and...

Claims

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Application Information

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IPC IPC(8): A61K36/068A61P35/00A61P35/04
CPCA61K36/068A61K2236/15A61K2236/331A61K2236/39A61K2236/51A61K2236/53
Inventor 钱正明肖瑛甄达明李春红蔡宏伟温宝庆陈荣升唐新发
Owner DONGGUAN HEC CORDYCEPS R&D CO LTD
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