Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Application of MT (Metallothionein) gene serving as target gene for preventing and treating AFB1 (Aflatoxin B1) caused hepatic injury of duck

A liver injury and genetic technology, applied in the field of prevention and treatment of duck liver injury target genes caused by AFB1, can solve the problems of unclear liver toxicity and other issues

Inactive Publication Date: 2017-06-20
HUAZHONG AGRI UNIV
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Duck AFB 1 Liver damage caused by poisoning has no effective treatment or mitigation methods, mainly due to which key genes are involved in AFB 1 Induced hepatotoxicity in ducks is unknown

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of MT (Metallothionein) gene serving as target gene for preventing and treating AFB1 (Aflatoxin B1) caused hepatic injury of duck
  • Application of MT (Metallothionein) gene serving as target gene for preventing and treating AFB1 (Aflatoxin B1) caused hepatic injury of duck
  • Application of MT (Metallothionein) gene serving as target gene for preventing and treating AFB1 (Aflatoxin B1) caused hepatic injury of duck

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: AFB 1 Changes of cellular antioxidant enzyme activity after treatment of normal duck primary hepatocytes and MT overexpression duck primary hepatocytes

[0018] The six-well plate was rinsed twice with cold PBS solution, and the hepatocytes of each treatment group were collected in 1.5mL EP tubes and frozen at -80°C. For experiments, the cells were triturated with liquid nitrogen and lysed with saline at 4°C. The activities of SOD, GST, GPX and other enzymes and the content of MDA were all operated according to the relevant instructions of Nanjing Jiancheng, and measured with an ultraviolet spectrophotometer.

[0019] It can be seen from the results that 50ng / mL AFB 1 After treatment of hepatocytes (AFB 1 group), the activities of GPX and SOD in cells were significantly lower than those without AFB 1 The treatment group (control group) (P1 The activities of GPX and SOD in the cells treated with MT overexpression hepatocytes group (MT+A group) were signifi...

Embodiment 2

[0020] Example 2: AFB 1 Changes of intracellular AFBO-DNA content in normal duck primary hepatocytes and MT overexpressed duck primary hepatocytes

[0021] The determination of AFBO-DNA adduct was performed according to the instructions of Aflatoxin DNA Adduct Competitive ELISA Kit (Cell Biolabs, Inc.) (AKR-351). (1) Standard curve preparation: The AFBO-DNA standard was serially diluted to prepare solutions with concentrations of 0, 0.0625, 0.125, 0.25, 0.5, 1, 2, and 4 μg / mL. (2) Add 50 μL of sample and AFBO-DNA standard to each well of the coated plate, and shake on a shaker at room temperature for 10 minutes. (3) Antibody dilution: Dilute AFBO-DNA primary antibody with Assay Diluent 1:500, immediately add 50 μL of the diluted solution to each well of the coated plate, and shake at room temperature for 1 hour. (4) Wash 3 times with washing solution, and absorb the residual liquid with filter paper. (5) After diluting the AFBO-DNA secondary antibody at a ratio of 1:1000, i...

Embodiment 3

[0023] Embodiment 3: MTT test measures AFB 1 Changes of cell viability after treatment of normal duck primary hepatocytes and MT overexpression duck primary hepatocytes

[0024] The duck primary hepatocytes in the logarithmic growth phase were digested with trypsin, and the primary hepatocyte suspension was made with WME medium. After adjusting the cell concentration, they were inserted into a 96-well plate to ensure that the number of cells per well was 2000~ 4000, placed in CO2 After the cells adhered to the wall in the incubator, the medium was discarded, and the medium containing AFB was added 1 100 μL of medium, AFB in each medium 1 Concentrations were 25, 50, 75, 100, 125ng / mL, placed in CO 2 Cultivate in the incubator for 24h. After the culture is over, add 10 μL of 0.5% MTT solution to each well of the culture plate, place it in the incubator for 4 hours, discard the medium carefully, add 150 μL of DMSO, shake on the shaker for 10 minutes, and use a microplate reade...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of an MT (Metallothionein) gene serving as a target gene for preventing and treating the AFB1 (Aflatoxin B1) caused hepatic injury of a duck. The pollution of AFB1 in feed is wide; after an animal ingests the AFB1, the production performance of the animal is caused to decrease, even to die. The AFB1 is mainly enriched in a hepatic organ of the animal, damages a hepatic function, and even induces cancerization. Protein coded by the MT gene can further inhibit the AFB1 induced cancerization and apoptosis of a hepatic cell through enhancing the antioxidant ability of the hepatic cell and regulating and controlling the expressions of a cancer inhibiting factor and an apoptosis promotion related gene; the AFB1 induced injury of primary hepatocyte of the duck can be effectively inhibited. According to the invention, a novel target gene is provided for developing a medicine for preventing and treating the AFB1 caused hepatic injury of the duck and developing a novel feed additive.

Description

technical field [0001] The invention relates to a new application of the MT gene, in particular to the application of the MT gene as a target gene for preventing and treating duck liver damage caused by AFB1. Background technique [0002] Aflatoxin B in Feed 1 (aflatoxin B 1 ,AFB 1 ) pollution is ubiquitous, which seriously damages the health of animals, reduces the production performance of animals, and causes aflatoxin residues in animal products, which brings great hidden dangers to the edible safety of animal products. Ducklings are common among livestock and poultry against AFB 1 One of the most sensitive species. Oral ingestion of AFB by animals 1 After that, it mainly accumulates in the liver, and at the same time, the liver is also the bioactivator of AFB 1 highly toxic AFB 1 - Outer 8,9-epoxide (AFBO) site, is AFB 1 The main target organ of the damage, and the liver injury of ducklings has typical pathological changes. Therefore, primary hepatocytes from du...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K48/00A23K50/75A61P1/16
CPCA61K48/005
Inventor 孙铝辉张妮娅齐德生高鑫赵玲
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com