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Method for impinging stream assisted extraction of collagen

A collagen and auxiliary extraction technology, applied in the field of bioengineering of medical materials, can solve the problems of long production cycle, low purity and biological activity of collagen, and achieve the effect of maintaining biological activity, shortening the production cycle, and simplifying the process flow

Active Publication Date: 2017-06-20
枣庄明杨生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above defects or improvement needs of the prior art, the present invention provides a method for extracting collagen, the purpose of which is to extract collagen by combining acid extraction and enzyme extraction, and by means of the technology of impacting flow assisted extraction, This solves the technical problems of long production cycle and low purity and biological activity of the extracted collagen in the prior art collagen extraction method

Method used

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  • Method for impinging stream assisted extraction of collagen
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Examples

Experimental program
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Effect test

Embodiment 1

[0038] Remove fascia, fat and other sundries from 500g of bovine Achilles tendon, wash, cut into small pieces of 1mm×1mm, add 5000ml of NaOH solution with a concentration of 0.2mol / L, soak for 7h, filter and wash with water until neutral; Take 20 g of the treated beef Achilles tendon, add 500 ml of acetic acid with a concentration of 0.5 mol / L and 2 g of pepsin, and then spray the annular liquid through a double-nozzle impingement flow device with a nozzle diameter of 0.1 mm and a flow rate of 20 m / s Membrane phase, make the system impact, the temperature of the reaction system is controlled at 4 ° C, the impact reaction time is controlled at 2.5 h, the extract is obtained, filtered, the supernatant is retained, the pH value is adjusted to neutral, and then NaCl is added to make NaCl The concentration of the solution was 1.5 mol / L, and after standing for 12 hours, it was filtered to collect the precipitate; the collected precipitate was washed and freeze-dried to obtain the col...

Embodiment 2

[0040] Get the bovine Achilles tendon after alkali treatment in 100g embodiment 1, add acetic acid and 20g pepsin that 3000 milliliters of concentrations are 1.0mol / L, then be that 3mm, flow velocity are the double nozzle impingement flow device of 10m / s by jet outlet diameter Spray the annular liquid film phase to make the system collide, control the temperature of the reaction system at 15°C, and control the colliding reaction time at 5h, obtain the extract, filter, keep the supernatant, adjust its pH value to neutral, and then add NaCl, the concentration of NaCl was 3 mol / L, and after standing for 16 hours, it was filtered to collect the precipitate; the collected precipitate was washed and freeze-dried to obtain the collagen extract.

Embodiment 3

[0042] Get the bovine Achilles tendon after alkali treatment in 200g embodiment 1, add acetic acid and 10g pepsin that 8000 milliliters of concentrations are 1.5mol / L, then be that 5mm, flow velocity are the double nozzle impingement flow of 0.5m / s by jet outlet diameter The device sprays out the ring-shaped liquid film phase to make the system collide. The temperature of the reaction system is controlled at 30°C, and the collision reaction time is controlled at 8h. The extract is obtained, filtered, and the supernatant is retained, and its pH value is adjusted to neutral, and then NaCl was added to make the concentration of NaCl 5 mol / L, and after standing for 24 hours, the precipitate was collected by filtration; the collected precipitate was washed and freeze-dried to obtain the collagen extract.

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Abstract

The invention discloses a method for impinging stream assisted extraction of collagen. The method mainly comprises the following steps: (1) treatment of fresh cattle achilles tendon with alkali; (2) accelerated extraction by adopting an impinging stream assisted method in the process of enzyme extraction of the collagen; (3) precipitation and separation of the collagen in an extract; and (4) washing and freeze-drying of the collagen. According to the invention, the collagen is extracted in an acidic aqueous solution under the actions of pepsin and impinging stream assisted external force, and a stable triple-helical structure of a collagen molecule is accelerated to be unwound into a single-helical structure, so the process of extracting the collagen is accelerated; and the technical process is simple and easy, improves the extraction rate and the purity of the collagen, shortens the production period, and maintains the biological activity of the collagen.

Description

technical field [0001] The invention belongs to the field of bioengineering of medical materials, and more specifically relates to a method for assisted extraction of collagen by impinging flow. Background technique [0002] Collagen is the most abundant fibrous and macromolecular protein in mammals, accounting for 25% to 30% of the total protein in the body. It is mainly distributed in the connective tissue of mammals and plays an important role in the formation of animal and human cartilage, skin and tendons. very important. A typical collagen molecule is a long, tough triple helix. At present, 27 different types of collagen have been discovered, which are called type I collagen, type II collagen, type III collagen, etc. according to the sequence of discovery, and are named with uppercase Roman numerals. [0003] Collagen has many excellent biological properties: the repetitive unit in the molecular structure of collagen is large, the immunogenicity is very low, and ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/30C07K1/14C12P21/06
CPCC07K14/78C12P21/06
Inventor 马兆成刘石林王蒙召左静
Owner 枣庄明杨生物科技有限公司
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