Bacillus siamensis and application thereof to prevention and treatment of powdery mildew of plant

A technology for bacillus and powdery mildew, applied in the field of microorganisms, can solve the problems of chemical agents polluting agricultural products and the environment, endangering the health of humans and animals, and reducing the control effect of chemical agents. The effect of avoiding pesticide residues and environmental pollution

Active Publication Date: 2017-06-20
昆明市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term and continuous use of chemical pesticides not only makes powdery mildew bacteria resistant, but also leads to a decline in the control effect of chemical pesticides year by year, and the residues of chemical pesticides seriously pollute agricultural products and the environment, endangering the health of humans and animals

Method used

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  • Bacillus siamensis and application thereof to prevention and treatment of powdery mildew of plant
  • Bacillus siamensis and application thereof to prevention and treatment of powdery mildew of plant
  • Bacillus siamensis and application thereof to prevention and treatment of powdery mildew of plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Isolation, screening and identification of Bacillus siamese X

[0022] 1. Isolation and screening of strains

[0023] 1. Collect strawberry fruits infected with powdery mildew grade 9 in the strawberry greenhouse of the self-cultivating manor on Guanyinshan Road, Xishan District, Kunming City, and pack them in sterilized plastic trays and bring them back to the laboratory;

[0024] 2. Open the plastic plate near the alcohol flame on the aseptic operating table, hold the strawberry fruit with sterilized tweezers, and lightly press the denser side of the white powder layer on the basic agar medium plate, and take another two strawberries in the same way Powdery mildew fruits were lightly pressed on PDA medium and LB medium plates respectively, and cultivated at room temperature (about 20°C);

[0025] After 3 or 6 days, several similar fungi grew on different plates. It was found that while several fungi grew on one LB medium plate, there was a strain next t...

Embodiment 2

[0041] Embodiment 2: Control test of Bacillus siamese X to the strawberry powdery mildew of potted strawberry

[0042] 1. Inoculate Bacillus siamese X in the liquid basal medium, cultivate it at 30°C and 160rpm for about 3 days, obtain the fermentation broth, spread and count, and the result is 10 9 More than cfu / mL, spare.

[0043] 2. Pot experiment

[0044] In the strawberry greenhouse of the self-cultivating farmer’s manor, 15 strawberry plants (pots) with flowers and fruits with severe powdery mildew and 3 healthy plants (pots) were selected and put together, numbered 1-18 and labeled. At the same time, record the powdery mildew situation of each strawberry, including the powdery mildew level and quantity of leaves and fruits;

[0045] ① Strawberry: There are 7 large leaves, many powdery mildew spots on the back, 3 leaves with grade 9 powdery mildew, 3 leaves with grade 7 powdery mildew, 1 leaf with grade 5, one big tidbit, 2 small fruits, The fruit is free from powdery...

Embodiment 3

[0063] Example 3: Control test of bacterial strain X fermentation liquid on strawberry powdery mildew in greenhouse field test

[0064] 1. Inoculate the strain X in the liquid basal medium, cultivate it at 30°C and 160rpm for about 3 days, obtain the fermentation broth, spread and count, and the result is 10 9 For more than cfu / mL, use 6kg of fermentation broth to 54kg of water each time, that is, dilute the original fermentation broth by 10 times for the following tests.

[0065] 2. The experiment was carried out in three strawberry plastic greenhouses owned by Dong, a strawberry grower in Fushan Village, Xishan District, Kunming City. Each plastic greenhouse is 48m long and 6m wide. Strawberries are cultivated in 8 ridges, and each ridge has about 860 strawberries. The incidence of powdery mildew is compared Heavy, strawberries are in full fruit stage, powdery mildew mostly occurs on fruits and flowers, and the disease index is 35%; test treatment: No. 1 shed is evenly spray...

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Abstract

The invention discloses bacillus siamensis X. The collection number of the bacillus siamensis X in the China Center for Type Culture Collection is CCTCC NO: M 2017008; a strain of the bacillus siamensis X is applied to the prevention and the treatment of the powdery mildew of the plant, compared with the existing chemical preventing and treating agent, the bacillus siamensis X is obvious in prevention and treatment effects, can be used for effectively controlling and relieving the state of an illness, can be used for making a disease plant recuperate health, further, is stable in the prevention and treatment effects, makes the recrudescence difficult, is safe, nontoxic, harmless and environment-friendly, and can be used for effectively avoiding pesticide-residue and environmental-pollution problems brought by the prevention and the treatment of a chemical pesticide; further, a preparation method of a product provided by the invention is simple, and is easy to operate; the bacillus siamensis X is suitable for industrialized production and market popularization and application.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a bacillus siamese and its application in preventing and treating plant powdery mildew. Background technique [0002] Powdery mildew is caused by fungi of the Powdery mildew family. It is a common plant disease. It occurs frequently on fruits and vegetables. It mainly damages the aboveground parts of plants, including leaves, stems, flower stalks, flower buds, petals, and fruits. Powdery mildew layer, the mildew spot is dispersed separately in the early stage, and the powder layer is thin, and then combined into a large mildew spot, the white powder layer is thick and numerous, and can even cover the whole leaves, flowers and fruits or the whole plant. In severe cases, it becomes infected in patches, seriously affecting photosynthesis The normal metabolism is disturbed, resulting in premature aging and production reduction. Generally, the lower leaves are more than the u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 张振荣童江云魏世杰包涛
Owner 昆明市农业科学研究院
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