A strain of Bacillus siamese and its application in the control of plant powdery mildew

A technology for bacillus and powdery mildew, applied in the field of microorganisms, can solve problems such as the decline in the control effect of chemical agents, endangering the health of humans and animals, and the pollution of agricultural products and the environment by chemical agents, and is suitable for industrial production and market promotion. The effect of avoiding pesticide residues and environmental pollution

Active Publication Date: 2019-11-05
昆明市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term and continuous use of chemical pesticides not only makes powdery mildew bacteria resistant, but also leads to a decline in the control effect of chemical pesticides year by year, and the residues of chemical pesticides seriously pollute agricultural products and the environment, endangering the health of humans and animals

Method used

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  • A strain of Bacillus siamese and its application in the control of plant powdery mildew
  • A strain of Bacillus siamese and its application in the control of plant powdery mildew
  • A strain of Bacillus siamese and its application in the control of plant powdery mildew

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Isolation, screening and identification of Bacillus siamese X

[0022] 1. Isolation and screening of strains

[0023] 1. Collect strawberry fruits infected with powdery mildew grade 9 in the strawberry greenhouse of the self-cultivating manor on Guanyinshan Road, Xishan District, Kunming City, and pack them in sterilized plastic trays and bring them back to the laboratory;

[0024] 2. Open the plastic plate near the alcohol flame on the aseptic operating table, hold the strawberry fruit with sterilized tweezers, and lightly press the denser side of the white powder layer on the basic agar medium plate, and take another two strawberries in the same way Powdery mildew fruits were lightly pressed on PDA medium and LB medium plates respectively, and cultivated at room temperature (about 20°C);

[0025] After 3 or 6 days, several similar fungi grew on different plates. It was found that while several fungi grew on one LB medium plate, there was a strain next t...

Embodiment 2

[0041] Embodiment 2: Control test of Bacillus siamese X to the strawberry powdery mildew of potted strawberry

[0042] 1. Inoculate Bacillus siamese X in the liquid basal medium, cultivate it at 30°C and 160rpm for about 3 days, obtain the fermentation broth, spread and count, and the result is 10 9 More than cfu / mL, spare.

[0043] 2. Pot experiment

[0044] In the strawberry greenhouse of the self-cultivating farmer’s manor, 15 strawberry plants (pots) with flowers and fruits with severe powdery mildew and 3 healthy plants (pots) were selected and put together, numbered 1-18 and labeled. At the same time, record the powdery mildew situation of each strawberry, including the powdery mildew level and quantity of leaves and fruits;

[0045] ① Strawberry: There are 7 large leaves, many powdery mildew spots on the back, 3 leaves with grade 9 powdery mildew, 3 leaves with grade 7 powdery mildew, 1 leaf with grade 5, one big tidbit, 2 small fruits, The fruit is free from powdery...

Embodiment 3

[0063] Example 3: Control test of bacterial strain X fermentation liquid on strawberry powdery mildew in greenhouse field test

[0064] 1. Inoculate the strain X in the liquid basal medium, cultivate it at 30°C and 160rpm for about 3 days, obtain the fermentation broth, spread and count, and the result is 10 9 For more than cfu / mL, use 6kg of fermentation broth to 54kg of water each time, that is, dilute the original fermentation broth by 10 times for the following tests.

[0065] 2. The experiment was carried out in three strawberry plastic greenhouses owned by Dong, a strawberry grower in Fushan Village, Xishan District, Kunming City. Each plastic greenhouse is 48m long and 6m wide. Strawberries are cultivated in 8 ridges, and each ridge has about 860 strawberries. The incidence of powdery mildew is compared Heavy, strawberries are in full fruit stage, powdery mildew mostly occurs on fruits and flowers, and the disease index is 35%; test treatment: No. 1 shed is evenly spray...

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Abstract

The invention discloses a bacillus siamese ( Bacillus siamensis ) X, its preservation number in the China Type Culture Collection Center is CCTCC NO: M 2017008, the strain of the present invention is used in the prevention and treatment of plant powdery mildew, compared with the existing chemical control agents, the control effect is remarkable, and it can quickly and effectively control And alleviate the disease, make the diseased plants recover quickly, and the control effect is stable, not easy to relapse, safe, non-toxic, harmless, environmental protection, effectively avoid the pesticide residue and environmental pollution problems caused by chemical pesticide control; and the product preparation method of the present invention Simple and easy to operate, it is suitable for industrial production and marketing application.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a bacillus siamese and its application in preventing and treating plant powdery mildew. Background technique [0002] Powdery mildew is caused by fungi of the Powdery mildew family. It is a common plant disease. It occurs frequently on fruits and vegetables. It mainly damages the aboveground parts of plants, including leaves, stems, flower stalks, flower buds, petals, and fruits. Powdery mildew layer, the mildew spot is dispersed separately in the early stage, and the powder layer is thin, and then combined into a large mildew spot, the white powder layer is thick and numerous, and can even cover the whole leaves, flowers and fruits or the whole plant. In severe cases, it becomes infected in patches, seriously affecting photosynthesis The normal metabolism is disturbed, resulting in premature aging and production reduction. Generally, the lower leaves are more than the u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 张振荣童江云魏世杰包涛
Owner 昆明市农业科学研究院
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