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Determination method of aflatoxin

A technology for the determination of aflatoxin and a method for measuring aflatoxin, which is applied in the field of determination of aflatoxin, and achieves the effects of easy promotion, accurate and reliable results, and simple operation

Inactive Publication Date: 2017-06-20
NANTONG BOTAI ART PATTERN DESIGN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current high-performance liquid chromatography analysis methods for aflatoxin all use immunoaffinity columns to enrich the toxin, and the shortcomings of the antibodies mentioned in the enzyme-linked immunoassay method are still unavoidable. Therefore, a new aflatoxin-rich method is studied. Set method to get rid of the shortcomings of antibodies

Method used

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  • Determination method of aflatoxin
  • Determination method of aflatoxin
  • Determination method of aflatoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Sample extraction: Weigh 10.0346g edible vegetable oil, add acetonitrile-water extract and stir for 45 minutes, wherein the volume ratio of acetonitrile and water is 20:12, and then filter with qualitative filter paper.

[0028] (2) Determination of standard solution: take aflatoxin B 1 , G 1 Standard stock solution 10μg / mL, dilute to 5mL with acetonitrile, mix well, store at 2-8°C for three months; use acetonitrile to prepare 0.025μg / mL, 0.05μg / mL, 0.1μg / mL, 0.15μg / mL , 0.2μg / mL, 0.25μg / mL series of standard solutions, absorb 500μL each of the standard series of solutions, blow dry with nitrogen in a water bath at 60°C, add 500mL of n-hexane and 100mL of trichloroacetic acid, mix well, and derivatize in an oven at 56°C for 15 minutes after 45 seconds , put it in a desiccator at room temperature for 1min, dissolve it with 500uL water-acetonitrile, put it into an auto-sampler bottle after mixing, and measure it with a liquid chromatograph. The curve of aflatoxin B1 ...

Embodiment 2

[0038] (1) Sample extraction: Weigh 10.1679g corn flour, add acetonitrile-water extract and stir for 45 minutes, wherein the volume ratio of acetonitrile and water is 20:12, and then filter with qualitative filter paper.

[0039] (2) Sample determination: detect aflatoxin in the sample with high performance liquid chromatography

[0040] Chromatographic conditions: chromatographic column: Agilent SB C-18 (25cm×4.6mm); mobile phase: 85% acetonitrile and 15% water by volume; column temperature: 45°C, injection volume: 25mL; flow rate: 1.0mL / min.

[0041] Take 8mL extract filtrate to Mycosep TM 228 multifunctional purification column, transfer 2mL of purification solution from the collection pool of the purification column to the derivatization bottle, blow with nitrogen at 60°C, add 500mL of n-hexane and 100mL of trichloroacetic acid to mix, after 45s, derivatize in an oven at 56°C for 15min, at room temperature After drying for 1min, dissolve it with 500uL water-acetonitrile...

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Abstract

The invention discloses a determination method of aflatoxin. The determination method is innovatively characterized by comprising the following steps: 1) weighing a sample and adding an acetonitrile-water extracting solution; stirring for 45min, wherein the volume ratio of acetonitrile to water is 20 to 12; then filtering with qualitative filter paper; 2) determining the sample, wherein chromatographic conditions are as follows: a liquid chromatographic column is Agilent SB C-18 (25cm*4.6mm), a mobile phase is prepared from 85 percent of the acetonitrile and 15 percent of the water according to a volume percent, column temperature is 45 DEG C, a sample feeding amount is 25mL and a flowing speed is 1.0mL / min; taking 8mL of an extracted filtering solution to a multifunctional purification column; transferring 2mL of a purified solution into a derivatization bottle from a collection tank of the purification column; after blowing nitrogen at 60 DEG C, adding 500mL of n-hexane and 100mL of trichloroacetic acid and uniformly mixing; after 45s, derivatizing for 15min in a drying oven at 56 DEG C; after drying at room temperature, dissolving with 500uL of acetonitrile-water; after uniformly mixing, conveying a mixed solution into an automatic sample feeding bottle and determining. The detection method disclosed by the invention is simple and rapid in operation, has an accurate and reliable result and has less pollution and high safety; pollution to operators and environment is reduced; the detection cost is relatively low and the determination method is easy to popularize.

Description

technical field [0001] The invention relates to a method for determining aflatoxin, and belongs to the technical field of food safety inspection. Background technique [0002] Aflatoxins (AF for short) are mainly metabolites of Aspergillus flavus and Aspergillus parasitica. Aflatoxins mainly exist in soil, animals and plants, and various nuts, especially peanuts and walnuts. Aflatoxins are also often found in soybeans, rice, corn, macaroni, condiments, milk, dairy products, cooking oil and other products. At present, 12 species have been isolated and identified, including B 1 , B 2 , G 1 , G2, M1, M2, P1, Q, H1, GM, B2a and toxic alcohol. [0003] There are three main methods for the analysis of aflatoxins: thin-layer chromatography, high-performance liquid chromatography, and enzyme-linked immunosorbent assay. Thin-layer chromatography is still used at home and abroad because of its simple equipment and easy popularization. However, due to the cumbersome sample pretre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 陈霞
Owner NANTONG BOTAI ART PATTERN DESIGN CO LTD