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Preparation method of ultralow-molecular-weight pectin rich in RG-I

A molecular weight, RG-I technology, applied in the field of polysaccharide degradation, can solve problems such as environmental pollution, limited degradation capacity, and reduced biological activity, and achieve the effects of environmental protection and pollution-free process conditions, improved comprehensive utilization, and simple degradation process.

Active Publication Date: 2017-06-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical degradation methods mainly include ultrasonic degradation, radiation degradation, microwave degradation, etc. The radiation method has the highest degradation efficiency, but the damage to the molecular structure is large, and the safety is controversial, so its application is limited; microwave degradation is easy to generate high temperature and affect the biological activity of degradation products ; Ultrasonic degradation has little damage to the active structure of the product, but its degradation ability is limited. It can only degrade pectin to about 30kDa, and it still cannot be absorbed by the human body.
Chemical degradation currently mainly includes acid degradation and alkali degradation, but due to the addition of strong acid, strong alkali, etc., it is easy to cause environmental pollution, the reaction rate is slow, and the reaction is generally carried out at high temperature, so the structure is greatly damaged and the biological activity is reduced.

Method used

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  • Preparation method of ultralow-molecular-weight pectin rich in RG-I
  • Preparation method of ultralow-molecular-weight pectin rich in RG-I
  • Preparation method of ultralow-molecular-weight pectin rich in RG-I

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Take 25mL distilled water, add 3mol / L HCl solution, adjust the pH to 3.0 with a pH meter, add FeCl 2 4H 2 O, configured as 0.5mmol / L Fe 2+ solution A;

[0024] (2) Dissolve 60 mg of pectin (Sigma company) in 25 ml of solution A, and after fully dissolving, transfer it to an ultrasonic tube (inner diameter 3 cm);

[0025] (3) Place it in a constant temperature bath at 30°C, place the ultrasonic probe at 1.5cm below the liquid surface for ultrasonic treatment, and add 50μL of 5mol / LH at the same time 2 o 2 solution, set the ultrasonic intensity to 1.5W / mL, and sonicate for 40 minutes;

[0026] (4) Add excess sodium bisulfite to the degradation solution to reduce excess hydrogen peroxide to terminate the reaction;

[0027] (5) passing the solution treated in step 4 through a cation exchange resin column, and controlling the flow rate to be 0.5mL / min, so that the cation exchange resin replaces the metal cations therein;

[0028] (6) Centrifuge the replaced solutio...

Embodiment 2

[0034] (1) Take 100mL distilled water, add 3mol / L HCl solution, adjust the pH to 4.0 with a pH meter, add FeCl 2 4H 2 O, configured as 2mmol / L Fe 2+ solution A;

[0035] (2) Dissolve 500 mg of pectin (Sigma company) in 100 ml of solution A, and after fully dissolving, transfer it to an ultrasonic tube (inner diameter 6 cm);

[0036] (3) Place it in a constant temperature bath at 30°C, place the ultrasonic probe at 1.5 cm below the liquid surface for ultrasonic treatment, and add 800 μL of high-concentration H 2 o 2 solution, set the ultrasonic intensity to 4.0W / mL, and sonicate for 30 minutes;

[0037] (4) Add excess sodium bisulfite to the degradation solution to reduce excess hydrogen peroxide to terminate the reaction;

[0038] (5) passing the solution treated in step 4 through a cation exchange resin column, and controlling the flow rate to be 1.0mL / min, so that the cation exchange resin replaces the metal cations therein;

[0039] (6) Centrifuge the replaced solutio...

Embodiment 3

[0046] (1) Take 1000mL distilled water, add 3mol / L HCl solution, adjust the pH to 5.0 with a pH meter, add FeCl 2 4H 2 O, configured as 5mmol / L Fe 2+ solution A;

[0047] (2) Get 10g pectin (Sigma company) and dissolve in 1000ml solution A, after fully dissolving, transfer to the ultrasonic tank;

[0048] (3) Place it in a constant temperature bath at 40°C, and add 20mL of high-concentration H 2 o 2 solution, set the ultrasonic intensity to 4.0W / mL, and sonicate for 30 minutes;

[0049] (4) Add excess sodium bisulfite to the degradation solution to reduce excess hydrogen peroxide to terminate the reaction;

[0050] (5) passing the solution treated in step 4 through a cation exchange resin column, and controlling the flow rate to be 2.0mL / min, so that the cation exchange resin replaces the metal cations therein;

[0051] (6) Centrifuge the replaced solution at 4000r / min, take the supernatant, and adjust the pH to 6.0;

[0052] (7) Transfer the above solution to a 500Da d...

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Abstract

The invention discloses a preparation method of an ultralow-molecular-weight pectin rich in RG-I. The preparation method comprises the steps that Fe<3+> generated in a reaction system is reduced into Fe<2+> through ultrasonic oxidation reaction, re-reaction between the Fe<2+> and hydrogen peroxide is strengthened to produce hydroxyl free radicals, oxidative cleavage of pectin carbohydrate chain is caused, and galacturonic acid in special attack pectin forms the low-molecular-weight pectin rich in RG-I structural domain. The weight-average molecular weight of the pectin is 5-10 kDa, esterification degree is reduced, the product is the ultralow-molecular-weight pectin rich in RG-I, the antioxidant activity is remarkably higher than that of original pectin, and the comprehensive utilization rate is improved.

Description

technical field [0001] The invention relates to a method for preparing ultra-low molecular weight pectin rich in RG-I, in particular to a method for rapidly preparing ultra-low molecular weight pectin which is green and rapid by ultrasonic-assisted oxidation, and belongs to the field of polysaccharide degradation. Background technique [0002] Pectin is mainly a kind of acidic heteropolysaccharide linked by D-galacturonic acid through α-1,4 glycosidic bonds, and its side chain contains a large amount of neutral sugars such as rhamnose, arabinose and galactose. Pectin mainly includes three structural domains: homogalacturonan (HG), rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II). It is widely found in plants, such as apples, citrus, guava, sunflower discs and sweet potato residues, and is considered to be a soluble dietary fiber, which has various functions such as lowering cholesterol, preventing diabetes, preventing obesity and maintaining intestinal health. ...

Claims

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Application Information

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IPC IPC(8): C08B37/06
CPCC08B37/0045
Inventor 陈士国支梓鉴叶兴乾陈健乐程焕刘东红丁甜
Owner ZHEJIANG UNIV
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