Magnetic stirring type drug sensitivity analysis apparatus and matched kit

An analytical instrument and magnetic stirring technology, which is applied in the field of magnetic stirring drug sensitivity analysis instruments and matching kits, can solve problems such as numerous operation steps, failure to meet the requirements of rapid detection, and full contact between unfavorable bacteria and drugs and nutrients

Pending Publication Date: 2017-07-04
朱红 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many steps
Moreover, the whole culture process has been in a static state, which is not conducive to the full contact of bacteria, drugs and nutrients, and is not conduc

Method used

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  • Magnetic stirring type drug sensitivity analysis apparatus and matched kit
  • Magnetic stirring type drug sensitivity analysis apparatus and matched kit
  • Magnetic stirring type drug sensitivity analysis apparatus and matched kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 provides the detection embodiment of final value method. Figure 6 A schematic diagram of the local structure of the supporting kit is given. Drug susceptibility testing by final value method is carried out according to the following steps.

[0034] Step 1, prepare the sample solution and inoculate the kit. Prepare a certain concentration of sample solution, and add the prepared sample solution to all wells.

[0035] Step two, cultivate. Put the prepared specimen kit into the incubator for 18-24 hours.

[0036] Step three, on-board testing. Figure 4 The detection flow chart of Example 1 is given. Step S11, start operation, enter step S12, the user selects the final value method detection mode through the input terminal of the microcomputer, the signal processing control unit receives the instruction from the microcomputer, sends a control signal, the stirring motor 105, and the motion motor 107 are in a static state , waiting for the next command. I...

Embodiment 2

[0038] Example 2 provides an example of monitoring during cultivation. First, prepare the sample solution and inoculate the kit, the steps are the same as in Example 1. Second, on-machine culture testing. Figure 5 The detection flow chart of Example 2 is given. Step S21, start running. Entering step S22, the user selects the monitoring mode through the input terminal of the microcomputer. The user enters the sample setting through the input terminal of the microcomputer, and enters step S23. The operation of setting and taking the sample is the same as the operation steps of setting and taking the sample in Embodiment 1. After the sample kit is set, go to step S24, the signal processing control unit controls the motion motor 107 to stop at the cultivation position, and the stirring motor 105 is located directly below the first row of drug-sensitive stirring holes. If the user takes out the sample kit, go to step S215. At this point, if the user inserts the specimen kit a...

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Abstract

The relates to the technical field of drug sensitivity analysis, and in particular to a magnetic stirring type drug sensitivity analysis apparatus and a matched kit. The magnetic stirring type drug sensitivity analysis apparatus provided by the invention, which is used in a mode of being matched with a microcomputer, is simple to operate, short in drug-sensitivity time and high in result accuracy. Drug resistance of microorganisms can be judged by virtue of a final value method, and in addition, growth status of the microorganisms can be monitored in a culture process; and furthermore, by adding a stirring function in the culture process, a propagation speed of the drug-resistant microorganisms can be accelerated, the time of an entire drug-sensitivity process can be shortened and a step of manual operation can be omitted. In the microorganism culture process, a stirring motor rotates so as to drive a magnet to rotate and a stirrer, under the action of a rotating magnetic field, becomes rotating, so that drugs, microorganism liquid and air in drug-sensitivity stirring pores are fully mixed. The propagation speed of the drug-resistant microorganisms is accelerated and the drug-sensitivity process is shortened. After the culture process is implemented for a period of time, the kit, which is driven by a motion device, is moved to a detection device; and through repetitive culture and detection, a growth curve of the microorganisms is finally obtained.

Description

technical field [0001] The invention relates to the technical field of drug sensitivity analysis, in particular to a magnetic stirring type drug sensitivity analysis instrument and a matching kit. Background technique [0002] Due to the increasing drug resistance of microorganisms, infectious diseases require accurate identification of pathogenic bacteria and testing their antimicrobial susceptibility (AST) in vitro, so as to provide reference for clinical use. At present, the clinic mainly relies on manual methods or commercial analysis systems for detection. Traditional manual methods and commercial analysis systems generally use the final value method to dilute the colonies to a certain multiple, add them to antibiotic kits coated with different types and concentrations, and put them in an incubator for 24 hours. Observe the kit with naked eyes The turbidity of the liquid in the small hole, or the way to judge the drug resistance of microorganisms by means of instrument...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/38C12M1/34C12M1/02
CPCC12Q1/18
Inventor 周成刚张敏张巍何芳陈奇斌
Owner 朱红
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