Application of cinnamaldehyde in preparing medicine for treating atherosclerosis

A technology of atherosclerosis and cinnamon aldehyde, which is applied in the preparation of cinnamon aldehyde in the preparation of drugs for the treatment of atherosclerosis and in the field of preparation of target organ protection drugs. Tolerance and other problems, to achieve the effect of reducing the content of macrophages

Active Publication Date: 2017-07-07
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the technical defects that the drugs for the treatment of atherosclerosis in the prior art have large toxic and side effects, patients cannot tolerate them, affect clinical curative effect and patients' confidence in treatment, and provide a new application of cinnamaldehyde in pharmacy, specifically related to Use of cinnamon aldehyde in preparation of medicine for treating atherosclerosis

Method used

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  • Application of cinnamaldehyde in preparing medicine for treating atherosclerosis
  • Application of cinnamaldehyde in preparing medicine for treating atherosclerosis
  • Application of cinnamaldehyde in preparing medicine for treating atherosclerosis

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1. Effect of cinnamaldehyde on aortic plaque

[0025] ApoE - / - Mice (purchased from Changzhou Cavens Experimental Animal Co., Ltd.) were fed with high-fat diet for 13 weeks. After 13 weeks, the mice without aortic plaque were removed by imaging screening, and the remaining mice were randomly divided into groups from the 13th week, and were given cinnamaldehyde (purchased from Shanghai Baoman Biotechnology Co., Ltd.) intervention, and continued to be fed for 13 weeks. The experiments were divided into: ① model group: fed with high-fat feed; ② high-dose cinnamon aldehyde group: fed with high-fat feed + 130 mg / kg / day cinnamaldehyde; ② low-dose cinnamon group: fed with high-fat feed + 32.5 mg / day kg / day. During the experiment, small animal ultrasound was used to regularly observe and record the formation of arterial plaques in mice, and blood was collected at 13, 17, 21, and 25 weeks, and heart tissue was collected at the end of the experiment. Then HE staining w...

Embodiment 2

[0027] Example 2. The effect of cinnamaldehyde on the content of macrophages in the plaque

[0028] Macrophages endocytose plaque lipid components through various scavenger receptors on the cell surface to form foam cells, release cholesterol crystals and inflammatory stimulators after necrosis, promote the formation of necrotic cores, and accelerate the death of adjacent cells and atherosclerosis Progress.

[0029] Immunohistochemistry was used to analyze the content of macrophages in the plaques after the hearts were collected. The methods were as follows: conventional xylene dewaxing and gradient hydration. 3%H 2 0 2Removal of endogenous peroxidase for high pressure repair. Normal goat serum was blocked, and the corresponding primary antibody was added overnight at 4°C. Add horseradish enzyme-labeled biotinylated secondary antibody and incubate at room temperature for 40 min. DAB color development (yellow for positive results), counterstaining with hematoxylin, transpa...

Embodiment 3

[0031] Example 3. Effect of cinnamaldehyde on smooth muscle cell content in plaque

[0032] Smooth muscle cells can secrete a variety of cell matrices, and are the only cell source of endocrine collagen in atherosclerotic plaques. Their main function is to repair the damaged cell matrix components and thus play a protective role.

[0033] After the heart was collected, the content of smooth muscle cells in the plaque was analyzed by immunohistochemistry, and the method was the same as in Example 2.

[0034] The result is as image 3 Shown, compared with model group, cinnamaldehyde 130mg / kg can significantly increase ApoE - / - The content of smooth muscle cells in mouse aortic plaques increased the source of collagen secretion and promoted the stability of plaques (p<0.05).

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Abstract

The invention relates to application of cinnamaldehyde in preparing a medicine for treating atherosclerosis and belongs to the technical field of traditional Chinese medicine. Pharmacodynamic experiments prove that cinnamaldehyde has no influence on the improvement of an aorta plaque area of an ApoE-/-mouse fed by high-fat feed, and can significantly reduce a macrophage content in a plaque, increase smooth muscle cell and collagen contents to significantly improve plaque stability, significantly reduce expression of M1 macrophage markers iNOS (inducible nitric oxide synthase) and TNF (tumor necrosis factor)-alpha, and significantly increase expression of M2 macrophage markers CD206 and FIZZ (found in inflammatory zone)-1; cinnamaldehyde is proven to inhibit inflammatory reaction of the atherosclerosis to stabilize the plaque through an immunomodulation approach, has a good effect of stabilizing the vulnerable plaque of the atherosclerosis; and the economical, effective, safe and reliable medicine with no toxic or side effects is provided for clinical treatment of the atherosclerosis.

Description

technical field [0001] The present invention relates to the use of cinnamon aldehyde in the preparation of target organ protection drugs, especially the use of cinnamon aldehyde in the preparation of drugs for treating atherosclerosis, which belongs to the technical field of traditional Chinese medicine. Background technique [0002] With the progress of society and the change of lifestyle, the incidence of cardiovascular diseases such as atherosclerosis (AS) is increasing year by year. According to incomplete statistics, the incidence of atherosclerosis in my country is close to 10%, and the incidence of people over 60 years old is close to 80%. Modern medical research shows that atherosclerosis is a systemic disease related to lipid metabolism disorders. The proliferation of fibrous matrix components gradually develops into atherosclerotic plaques, leading to arteriosclerosis. When atherosclerosis occurs in important organs such as the heart, brain, and kidneys, it will ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/11A61P9/10A61P29/00A61P37/06A61P39/00
CPCA61K31/11
Inventor 郭齐路永刚王志国苏兆亮许化溪张春娇
Owner JIANGSU UNIV
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