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Method of purifying yeast wastewater by co-culture of chlorella and yeast

A technology of yeast wastewater and chlorella, applied in the field of cell culture

Active Publication Date: 2017-07-07
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned studies belong to indoor co-cultivation of microorganisms, small-scale treatment of wastewater

Method used

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  • Method of purifying yeast wastewater by co-culture of chlorella and yeast
  • Method of purifying yeast wastewater by co-culture of chlorella and yeast
  • Method of purifying yeast wastewater by co-culture of chlorella and yeast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: investigate the inoculation ratio of Chlorella pyrenoidosa and Rhodotorula viscosus

[0059] Experimental steps:

[0060] 1) Inoculate the cells of Chlorella pyrenoidosa and Rhodotorula viscosus into 250ml Erlenmeyer flasks equipped with improved basal medium and YM medium respectively, and place them in a constant temperature shaker at 28°C, 150r / m, and a light intensity of 4000lux , continuously cultivated for 6 days to obtain Chlorella pyrenoidosa seed liquid I and Rhodotorula viscosus seed liquid I.

[0061] Improved basal medium: NaNO 3 3750mg / L, KH 2 PO 4 1250mg / L, MgSO 4 ·7H 2 O 1000mg / L, EDTA500mg / L, H 3 BO 3 114.2mg / L, CaCl 2 2H 2 O 111mg / L, FeSO 4 ·7H 2 O49.8mg / L, ZnSO 4 ·7H 2 O88.2mg / L, MnCl 2 4H 2 O 14.2mg / L, Na2 M0O 4 2H 2 O 11.92mg / L, CuSO 4 ·5H 2 O 15.7mg / L, Co(NO 3 ) 2 ·6H 2 O 4.9mg / L, C 6 h 12 o 6 50g / L. Adjust the pH to 6.10.

[0062] YM medium: casein peptone 5.0g / L, malt extract powder 3.0g / L, glucose 10.0g / L,...

Embodiment 2

[0071] Embodiment 2: Step 3) Adopt preferred scheme one, Chlorella pyrenoidosa and Rhodotorula viscosus co-cultivate and process yeast wastewater in an outdoor 700L pipeline photobioreactor

[0072] Experimental steps:

[0073] 1) Inoculate the cells of Chlorella pyrenoidosa and Rhodotorula viscosus into 250ml Erlenmeyer flasks equipped with improved basal medium and YM medium respectively, and place them in a constant temperature shaker at 28°C, 150r / m, and a light intensity of 4000lux , continuously cultivated for 6 days to obtain Chlorella pyrenoidosa seed liquid I and Rhodotorula viscosus seed liquid I.

[0074] Improved basal medium: NaNO 3 3750mg / L, KH 2 PO 4 1250mg / L, MgSO 4 ·7H 2 O 1000mg / L, EDTA500mg / L, H 3 BO 3 114.2mg / L, CaCl 2 2H 2 O 111mg / L, FeSO 4 ·7H 2 O 49.8mg / L, ZnSO 4 ·7H 2 O88.2mg / L, MnCl 2 4H 2 O 14.2mg / L, Na 2 MoO 4 2H 2 O 11.92mg / L, CuSO 4 ·5H 2 O 15.7mg / L, Co(NO 3 ) 2 ·6H 2 O 4.9mg / L, C 6 h 12 o 6 50g / L. Adjust the pH to 6.10. ...

Embodiment 3

[0083] Embodiment 3: Step 3) Adopt preferred scheme two, Chlorella pyrenoidosa and Rhodotorula viscosus co-cultivate and process yeast wastewater in an outdoor 1300L pipeline photobioreactor

[0084] Experimental steps:

[0085] 1) Inoculate the cells of Chlorella pyrenoidosa and Rhodotorula viscosus into 250ml Erlenmeyer flasks equipped with improved basal medium and YM medium respectively, and place them in a constant temperature shaker at 28°C, 150r / m, and a light intensity of 4000lux , continuously cultivated for 6 days to obtain Chlorella pyrenoidosa seed liquid I and Rhodotorula viscosus seed liquid I.

[0086] Improved basal medium: NaNO 3 3750mg / L, KH 2 PO 4 1250mg / L, MgSO 4 ·7H 2 O 1000mg / L, EDTA500mg / L, H 3 BO 3 114.2mg / L, CaCl 2 2H 2 O 111mg / L, FeSO 4 ·7H 2 O 49.8mg / L, ZnSO 4 ·7H 2 O88.2mg / L, MnCl 2 4H 2 O 14.2mg / L, Na 2 MoO 42H 2 O 11.92mg / L, CuSO 4 ·5H 2 O 15.7mg / L, Co(NO 3 ) 2 ·6H 2 O 4.9mg / L, C 6 h 12 o 6 50g / L. Adjust the pH to 6.10. ...

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Abstract

The invention provides a method of purifying yeast wastewater by co-culture of chlorella and yeast. The method comprises the following steps: 1) performing activated culture on chlorella and yeast cells to obtain a chlorella seed liquid I and a yeast seed liquid I; 2) inoculating the chlorella seed liquid I into an improved basic culture medium, inoculating the yeast seed liquid I into a culture medium, and placing the media outdoors for culturing the seed liquids to obtain a chlorella seed liquid II and a yeast seed liquid II; and 3) dipping the chlorella seed liquid II to the yeast seed liquid II and inoculating the same into yeast wastewater needing to be treated to form a co-culture system for outdoor co-culture. Through three simple steps, the energy consumption and production cost of co-culture of chlorella and yeast can be effectively reduced by using a small amount of raw materials by means of light energy in a natural condition, and the yeast wastewater is treated on a large scale by means of microbial co-culture.

Description

technical field [0001] The invention belongs to cell culture technology, relates to microalgae and yeast co-cultivation technology, and mainly relates to a method for purifying yeast wastewater by co-culturing chlorella and yeast. Background technique [0002] Yeast wastewater is a kind of refractory, dark and high-concentration organic wastewater produced in the yeast production process, with high content of COD and BOD 5 , total nitrogen, total phosphorus, non-biodegradable organic pollutants and high chroma. With the expansion of the scale of biopharmaceutical factories, the amount of yeast fermentation wastewater produced has increased, causing great pollution to the environment. Research on efficient and environmentally friendly treatment methods is crucial to the sustainable development of the yeast industry. [0003] Chlorella is an algal species widely used in urban wastewater treatment. It has the advantages of fast growth rate, strong tolerance, and high protein ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/16C02F3/34C02F3/32C12R1/645C12R1/74
CPCC02F3/322C02F3/347C02F2209/06C12N1/12C12N1/16
Inventor 魏东张会贞
Owner SOUTH CHINA UNIV OF TECH
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