Method used for detecting mercury ions via DNA chain hybridization and surface enhanced Raman technology
A technology of surface-enhanced Raman and DNA strands, which is applied in the field of mercury ion detection, can solve the problems such as the inability to detect mercury ions, and achieve the effects of lower detection limit, high sensitivity and low interference
Inactive Publication Date: 2017-07-14
QINGDAO UNIV OF SCI & TECH
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[0005] Based on the shortcomings of the prior art, the present invention provides a method for detecting mercury ions through DNA strand hybridization and surface-enhanced Raman technology, which effectively solves the problem that low-concentration mercury ions cannot be detected, and ensures the accuracy of detection
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[0039] Such as Figure 1-2 As shown, the present invention is based on T-Hg 2+ -T's specificity and its stable binding ability, the introduction of mercury ions as target molecules, the adhesion of a large number of signal molecules through nanomaterial barcodes, the strong binding force of streptavidin and biotin, and the DNA hybridization of cycles The chain reaction amplifies the Raman signal, thereby reducing the detection limit and improving the sensitivity and accuracy. The specific detection method is as follows:
[0040] (1) Synthesis of gold nanoparticles
[0041] First, use aqua regia to soak the container used for the synthesis of gold nanoparticles for more than 24 hours to prevent pollution, then use deionized water to clean, dry and seal for storage for later use. When synthesizing gold nanoparticles, get 50ml concentration and take 1% sodium citrate microporous filtration to remove impurities for subsequent use; measure 500 μl concentration and add 1% chloroau...
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Abstract
The invention discloses a method used for detecting mercury ions via DNA chain hybridization and surface enhanced Raman technology. According to the method, capture chain DNA is connected with magnetic microspheres, stable connection of target chain DNA is realized by a T-Hg<2+>-T structure formed among the capture chain DNA, mercury ions, and target chain DNA; combination points of bio-barcodes are increased via trigger chain DNA fixing and hairpin DNA chain circular amplification hybridization chain reaction, and the bio-barcodes are connected with the magnetic microspheres via the relatively high combination capacity between biotin connected with hairpin DNA and streptavidin connected with the bio-barcodes, so that Raman single amplification is realized, and mercury ion detection limit is reduced. The interference of the method is low; selectivity is high; detection sensitivity on mercury ions is high; and accuracy is high.
Description
technical field [0001] The invention relates to the field of mercury ion detection, in particular to a method for detecting mercury ions through DNA chain hybridization and surface enhanced Raman technology. Background technique [0002] As a common metal, mercury is widely used in industry, but the pollution it causes is very harmful to human body and environment. Due to the volatility of mercury element, it can diffuse in water and air through various channels, and be oxidized into divalent mercury ions; mercury ions will cause serious neurotoxicity, genotoxicity and immunotoxicity when ingested by the human body; The environmental and health problems caused by mercury ions have also promoted the development of testing methods, and people have begun to try various methods for quantitative analysis of mercury ions in aqueous solutions. [0003] The traditional methods for detecting mercury ions mainly include chemical colorimetry and atomic absorption spectrometry, which h...
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IPC IPC(8): C12Q1/68G01N21/65
CPCG01N21/658C12Q1/682G01N2201/06113C12Q2563/143C12Q2563/149
Inventor 丁彩凤徐玉娟张瑞元
Owner QINGDAO UNIV OF SCI & TECH