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Treatment method of riboflavin fermentation liquid

A processing method and riboflavin technology are applied in the processing field of riboflavin fermentation broth, can solve the problems of low filtration efficiency, inconvenient cleaning, low solid content of filter cake and the like, achieve simple operation, high separation efficiency and save production cost Effect

Active Publication Date: 2017-07-18
HUBEI GUANGJI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because the ordinary plate and frame filter press has extremely low filtration efficiency and low dehydration efficiency for highly viscous organic substances such as riboflavin fermentation liquid cells, the filter residue is easy to stick to the filter plate, and the solid content of the filter cake is not high. Inconvenient to wait

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  • Treatment method of riboflavin fermentation liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 40,000L riboflavin fermentation broth was subjected to solid-liquid separation by high-efficiency centrifugation. Before separation, the morphology of riboflavin crystals and the morphology of bacteria were observed under a microscope. The riboflavin content in the mature riboflavin fermentation broth was 10779mg / L. Before centrifugation, the viscosity of the riboflavin fermented liquid is adjusted to about 130mpa, and the centrifugal speed is set at room temperature to be 3600 rpm. After separation, about 450L of concentrated riboflavin heavy phase was obtained, and about 33550L of fermented liquid cell light phase containing 600ppm riboflavin was obtained. The light phase of the fermentation liquid cell was observed under a microscope, and the basic morphology of riboflavin cell was basically the same as that before separation. Keeping consistent, the enrichment state of riboflavin cells is very good, and the riboflavin cells did not cause decomposition, breakage, etc....

Embodiment 2

[0044] 60,000L riboflavin fermentation liquid was subjected to high-efficiency centrifugation for solid-liquid separation. Before separation, the morphology of riboflavin crystals and the morphology of bacteria were observed under a microscope. The riboflavin content in the mature riboflavin fermentation liquid was 10385mg / L. The centrifugation process is the same as in Example 1. After separation, the concentrated about 700L riboflavin heavy phase is obtained, and about 59300L of the fermented liquid thallus light phase containing 500ppm riboflavin is observed under a microscope. The basic shape of the cells was basically the same as that before separation, and the enrichment state of the riboflavin cells was very good, and no decomposition or breakage of the riboflavin cells was caused. The light phase of the 59300L fermented liquid thallus contains about 1500kg of thalline, and the COD content is 58000mg / L. Sodium hydroxide aqueous solution is added to the light phase of the...

Embodiment 3

[0049] This implementation example adopts a kind of common plate and frame press filter to filter the fermented liquid thalline after concentration, is the comparative example of implementation example 2,

[0050] 60000L riboflavin fermentation broth was subjected to high-efficiency centrifugation for solid-liquid separation, and the riboflavin content in the mature riboflavin fermentation broth was 10385mg / L. The centrifugation treatment is the same as in Example 1. After separation, the concentrated heavy phase of about 700L riboflavin is obtained, and about 59300L of the fermented liquid thallus light phase containing 500ppm riboflavin is obtained. , COD content is 58000mg / L, in the fermented liquid thalline of this 59300L, add sodium hydroxide aqueous solution to regulate this fermented liquid thalline aqueous pH value to be 8-9, in this implementation example concrete pH is 8.9, in the situation of stirring Next, add the flocculant polyacrylamide anion PAM3000L with a mas...

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Abstract

The invention discloses a treatment method of a riboflavin fermentation liquid, and belongs to the technical field of riboflavin production. The method comprises the following steps of carrying out solid-liquid separation on the riboflavin fermentation liquid by adopting a centrifugal mode to obtain wastewater containing 500-800ppm of riboflavin and 2-3wt% of thallus; adjusting the pH value of the wastewater obtained through solid-liquid separation to be 8-9, adding an anionic flocculant which is 0.005-0.01% of weight of the wastewater, stirring, dissolving, standing and precipitating, discharging a supernatant liquid to obtain a fermented bacterial concentrated solution; adjusting the pH value of the fermented bacterial concentrated solution obtained through precipitating and separation to be 5-7 and carrying out inner dehydration by adopting a stirring mode; carrying out filter pressing on the liquid obtained through inner dehydration under relatively low feed pressure and carrying out high-pressure squeezing under relatively high squeezing pressure after filter pressing is completed; and drying a filter cake obtained through high-pressure squeezing to obtain a by-product rich in an organic matter.

Description

technical field [0001] The invention belongs to the technical field of riboflavin production, in particular to a method for processing riboflavin fermentation liquid. Background technique [0002] Riboflavin, also known as vitamin B2, is an essential water-soluble vitamin for the human body. Riboflavin has a wide range of physiological functions and can be synthesized in microorganisms and higher plants, but animals and humans cannot and must be ingested from food. Therefore, it is listed by the World Health Organization (WHO) as the sixth element for evaluating human growth and nutritional status. It is one of the major indicators, and it has important value in the fields of clinical medicine, feed processing, food industry and cosmetics manufacturing. [0003] The preparation of riboflavin by microbial fermentation is a very economical and effective method. Compared with other methods for preparing riboflavin, the preparation of riboflavin by microbial fermentation has lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F9/14A23K10/12C10L5/48C05G3/00
CPCC02F1/38C02F1/56C02F1/66C02F3/02C02F3/28C02F9/00C05F7/005C05G3/00C10L5/48
Inventor 郭韶智陈志远伍文俊黎艳华柯伯雄
Owner HUBEI GUANGJI PHARMA
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