Construction method of cell scaffold for repairing avascular area meniscus injuries

A technology of cell scaffold and construction method, which is applied in the field of tissue engineering technology to repair meniscus injury in bloodless area, can solve the problems such as poor treatment effect, and achieve the effect of easy adsorption of cells, shortening the culture period, and maintaining differentiation potential

Inactive Publication Date: 2017-07-18
安徽安龙基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the defect of poor therapeutic effect in the prior art, and to provi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The invention discloses a method for constructing a cell scaffold for repairing meniscus damage in an avascular area, and the specific steps are as follows:

[0051] (1) Under sterile conditions, use a 20ml syringe containing 1ml heparin sodium saline to draw out 9ml of the patient's autologous bone marrow. After removing the needle, add it to a culture bottle containing 10ml of DMEM high-glucose medium, and mix well to obtain the bone marrow. The suspension is ready for use, and then under aseptic conditions, take 180ml of peripheral blood from the patient, put it in a blood bag, and seal it for use;

[0052] (2) Put the peripheral blood in a 50ml centrifuge tube, centrifuge at 3000rpm for 30min, take the supernatant, then centrifuge the supernatant at 3000rpm for 30min, remove the precipitate, and filter through a pore size of 0.22μm to sterilize , prepare the autologous serum, and pack it for use;

[0053] (3) Slowly add the bone marrow suspension into the centrifug...

Embodiment 2

[0068] The invention discloses a method for constructing a cell scaffold for repairing meniscus damage in an avascular area, and the specific steps are as follows:

[0069] (1) Under sterile conditions, use a 20ml syringe containing 1ml heparin sodium saline to draw out 9ml of the patient's autologous bone marrow. After removing the needle, add it to a culture bottle containing 10ml of DMEM high-glucose medium, and mix well to obtain the bone marrow. The suspension is ready for use, and then under aseptic conditions, take 105ml of peripheral blood from the patient, put it in a blood bag, and seal it for use;

[0070] (2) Put the peripheral blood in a 50ml centrifuge tube, centrifuge at 3000rpm for 30min, take the supernatant, then centrifuge the supernatant at 3000rpm for 30min, remove the precipitate, and filter through a pore size of 0.22μm to sterilize , prepare the autologous serum, and pack it for use;

[0071] (3) Slowly add the bone marrow suspension into the centrifug...

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PUM

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Abstract

The invention discloses a construction method of a cell scaffold for repairing avascular area meniscus injuries. The construction method comprises the specific steps: taking a bone marrow suspension solution for later use; taking peripheral blood of a patient and sealing for later use; sub-packaging autoserum for later use; carrying out gradient centrifuging on the bone marrow suspension solution to obtain a cell suspension solution; culturing the cell suspension solution in a culture box; re-suspending cells by utilizing 0.5ml of a saline solution; taking one piece of a bovine derived type I collagen protein film; dripping the cell suspension solution into the bovine derived type I collagen protein film; immersing the bovine derived type I collagen protein film into the saline solution; and putting the bovine derived type I collagen protein film into a culture box which has a temperature of 37 DEG C and contains 5% of CO2 and incubating for 6h. The cell scaffold disclosed by the invention has the advantages of good treatment effect and the like.

Description

technical field [0001] The invention relates to the field of repairing meniscus damage in the avascular area by tissue engineering technology, in particular to a method for constructing a cell scaffold for repairing the meniscus damage in the avascular area. Background technique [0002] The meniscus of the knee joint is an important part of the structure and function of the knee joint. In addition to absorbing shocks, increasing the joint contact surface, and lubricating the joints, it also has the functions of bearing and distributing loads and maintaining joint stability. The removal of the meniscus will lead to joint instability. Stability and load transmission disorder, resulting in the occurrence of joint osteoarthritis. The meniscus is located between the femur and the tibia, one inside and one inside. It is fibrocartilage tissue, which is thick in the periphery and thin in the inner edge. It looks like a half moon on the plane. The outer 1 / 3 of the meniscus close to ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61L27/38A61L27/22
Inventor 韦玉军李航陆宝石苏军吴远航
Owner 安徽安龙基因科技有限公司
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