Preparation method of chitin nano-fiber composite film immobilized enzyme

A chitosan nano-fiber composite technology, applied in the direction of being fixed on/in an organic carrier, can solve the problems of low enzyme load and catalytic efficiency, reduced enzyme activity, and difficulty in recycling the immobilized enzyme carrier material, etc. To achieve the effect of improving biological activity and stability, sufficient sources, and improving the utilization rate of enzymes

Inactive Publication Date: 2007-04-18
ZHEJIANG UNIV
View PDF5 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the non-biospecific interaction between some carriers used for immobilizing enzymes and enzymes, which easily leads to the reduction of enzyme activity, as well as the low enzyme loading and catalytic efficiency of immobilized enzymes, and the dif

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example

[0020] example 1

[0021] Immerse 1 gram of chitosan nanofiber composite membrane in a 2% glutaraldehyde aqueous solution, and oscillate for 3 hours at a constant temperature of 25°C after sealing, with an oscillation speed of 40 revolutions per minute; take the nanofiber composite membrane and use the pH to 7.0 Rinse the phosphate buffer solution sufficiently to remove the residual glutaraldehyde on the membrane; immerse the nanofiber composite membrane activated by glutaraldehyde in a lipase phosphate buffer solution with a concentration of 2 mg / ml, and seal it at 4 Shake for 2 hours at a constant temperature of ℃, and the shaking speed is 40 revolutions per minute; take out the membrane from the solution and wash it repeatedly with deionized water for a total of 3 times to obtain the lipase-immobilized chitosan nanofiber composite; The nanofiber composite membrane with immobilized enzyme is immersed in a phosphate buffer solution with a pH of 7.0, and stored at 4°C for later us...

Example

[0022] Example 2

[0023] Immerse 1 gram of chitosan nanofiber composite membrane in a 10% glutaraldehyde aqueous solution. After sealing, oscillate for 5 hours at a constant temperature of 25°C at a speed of 60 revolutions per minute; take the nanofiber composite membrane and use the pH to 7.0 Rinse the phosphate buffer solution sufficiently to remove the residual glutaraldehyde on the membrane; immerse the nanofiber composite membrane activated by glutaraldehyde in a lipase phosphate buffer solution with a concentration of 6 mg / ml, and seal it at 4 Vibrate for 4 hours at a constant temperature of ℃, and the vibration speed is 60 revolutions per minute; remove the membrane from the solution, wash it repeatedly with deionized water for a total of 4 times to obtain the lipase-immobilized chitosan nanofiber composite; The nanofiber composite membrane with immobilized enzyme is immersed in a phosphate buffer solution with a pH of 7.0, and stored at 4°C for later use.

Example

[0024] Example 3

[0025] Immerse 1 gram of chitosan nanofiber composite membrane in a 5% glutaraldehyde aqueous solution. After sealing, oscillate for 8 hours at a constant temperature of 25°C at a speed of 80 revolutions per minute; take the nanofiber composite membrane and use the pH to 7.0 Rinse the phosphate buffer solution sufficiently to remove the residual glutaraldehyde on the membrane; immerse the glutaraldehyde-activated nanofiber composite membrane in a catalase phosphate buffer solution with a concentration of 8 mg / ml, and seal it Shake for 6 hours at a constant temperature of 4°C at a shaking speed of 80 revolutions per minute; remove the membrane from the solution and wash it repeatedly with deionized water for a total of 5 times to obtain catalase-immobilized chitosan Nanofiber composite: Immerse the nanofiber composite membrane with immobilized enzyme in a phosphate buffer solution with a pH of 7.0, and store it at 4°C for use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

This invention relates to using chitosan nanometer fabric compound membrane for the immobilized enzyme. It soluts chitosan and polyvinyl alcohol in mixed solvents of acetic acid and water to form a transparent solution, through static electricity spinning to form chitosan nanometer fabric compound membrane, and take it as immobilized enzyme carriers, through chemical bonding in ways to fix enzyme on composite membrane. The invention uses nanometer composite membrane of high specific surface area and high porosity, and chitosan which is the reactive amino biocompatibility and can be set with high-volume, high specific activity, high catalytic efficiency of chitosan nanometer fabric compound membrane immobilized enzyme.

Description

technical field [0001] The invention relates to a technique for immobilizing enzymes with a chitosan nanofiber composite membrane. Background technique [0002] Enzyme is a special kind of protein produced by living cells with high-efficiency catalytic function, high specificity and high controllability. It can specifically promote a certain chemical reaction without participating in the reaction itself. The reaction product has the characteristics of less pollution, low energy consumption, and easy control of the reaction, so it has been widely used. However, it is prone to denaturation of the enzyme protein under conditions such as acid, alkali, heat and organic solution, thereby reducing or losing the enzyme activity, and the enzyme reaction is mostly carried out in the solution, which is not easy to recover after the reaction is completed, and the separation and purification of the reaction product is difficult. And it is difficult to achieve continuous and automatic pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N11/12
Inventor 黄小军徐志康
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap