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Automatic micro-sampling device and control method for electrophoresis detector

A technology of micro-sampling and detectors, which is applied in the direction of measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problem of different sample injection methods of electrophoresis detectors, the inability to establish standardized tests, and the inability to horizontally compare the tests of different instruments Results and other problems to achieve the effect of eliminating test results, rapid on-site detection, and ensuring accuracy and repeatability

Active Publication Date: 2019-03-29
合肥中科方舟机器人技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, capillary electrophoresis detectors mostly use motorized sample injection, while microfluidic electrophoresis detectors mostly use pressure injection. Different types of electrophoresis detectors have different sample injection methods, which also makes it impossible to compare the test results of different instruments horizontally. Unable to establish standardized tests

Method used

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  • Automatic micro-sampling device and control method for electrophoresis detector
  • Automatic micro-sampling device and control method for electrophoresis detector
  • Automatic micro-sampling device and control method for electrophoresis detector

Examples

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example 1

[0082] Example 1, the detection of inorganic cations based on non-contact capillary electrophoresis instrument

[0083] The fused silica capillary was used as the electrophoresis pipeline, and the concentration of inorganic cations in the unknown aqueous solution was tested by a non-contact conductivity detector.

[0084] The specific operation process is as follows:

[0085] 1) Preparation of buffer solution: Weigh 2.8-3.2 g of Tris (hydroxymethyl) aminomethane, Tris, and 0.16-0.20 g of ethylenediaminetetraacetic acid (EDTA) into a clean beaker, add Fully dissolve in ultrapure water, then add 0.25-0.35mL of glacial acetic acid (0.2mol / L), transfer to a 50ml volumetric flask, and dilute to volume with ultrapure water. Weigh 0.50-0.70 g of Polyvinyl pyrrolidone (PVP) into a 50 mL clean beaker, transfer to a 100 mL volumetric flask, add 2 mL of Tris-EDTA solution, and dilute to volume with ultrapure water. Take 40mL of the prepared buffer solution to the buffer solution bottle...

example 2

[0093] Example 2, Antibiotic Detection Based on Microfluidic Electrophoresis Chip

[0094] When the microfluidic electrophoresis chip is used, the end of the sample pool of the microfluidic sampling module is connected with the microfluidic outlet module to form an integral manufacturing mold during design and manufacture. Fluorescence intensity of cephradine after electrophoresis separation.

[0095] 1) Prepare borax-sodium hydroxide buffer solution: wherein the concentration of borax is 5.75-6.50 mmol / L, pH=9.3. Take the prepared buffer solution to the buffer solution bottle of the automatic sampling device.

[0096] 2) prepare the cephradine aqueous solution, and inject the sample solution into the sample bottle.

[0097] 3) Insert the clean capillary and the chemically inert tube into the corresponding interfaces of the microfluidic sampling module and the microfluidic sampling module respectively, place them on the lower layer of the PMMA double-layer support, and fix t...

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Abstract

The invention relates to an automatic trace sample feeding device of an electrophoresis detector and a manufacturing method and control method of the automatic trace sample feeding device. The automatic trace sample feeding device comprises a microfluid sample feeding module, a microfluid sample discharging module, a first micro electromagnetic driving pump, a second micro electromagnetic driving pump, a first micro partitioning valve, a second micro partitioning valve, a high-voltage power supply, a sample bottle, a buffer liquid bottle, a sample waste liquid bottle, a waste buffer liquid bottle, a control circuit board, a control system, a storage battery and a double-layer bracket. The automatic trace sample feeding device is applicable to automatic trace sample feeding of a portable capillary electrophoresis detector and a microfluid electrophoresis chip, precise, automatic and repeated sample injection is achieved, operation errors are reduced, the operation efficiency is improved, artificial interference is avoided, a uniform sample feeding amount of each time is ensured, meanwhile a rapid capillary tube replacing interface is available, and sample pollution caused by the situation that a capillary tube is not easily cleaned on site is avoided.

Description

technical field [0001] The invention relates to the technical field of electrophoresis detectors, in particular to an automatic micro-sampling device for a portable electrophoresis detector and a control method thereof. Background technique [0002] Over the past few decades, electrophoresis detection has become a major analytical tool, with the advantages of fast detection speed, high precision, and less sample consumption. It has been widely used in many fields such as environmental monitoring, drug analysis, agricultural production, and food quarantine. Electrophoresis detectors are gradually developing towards miniaturization and portability. At present, portable electrophoresis detectors are mainly capillary electrophoresis instruments and microfluidic electrophoresis chips. Because the portable electrophoresis detector has the characteristics of small size, easy detection of trace samples, and shorter detection time, the application range of this powerful analysis tech...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447
CPCG01N27/44743G01N27/44791
Inventor 陈翔宇王儒敬高钧张俊卿汪六三郭红燕张正勇鲁翠萍宋良图魏圆圆刘洋朱利凯汪玉冰黄河
Owner 合肥中科方舟机器人技术有限公司
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