Chimeric tissue engineering bovine pericardium sheet
A tissue engineering and chimeric technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as immune rejection, tissue calcification, and epitope exposure
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[0006] Specific implementation methods Triton X-100 + deoxycholic acid sodium salt + EDTA decellularization method can completely remove the cells in the tissue and at the same time remove the matrix components in the tissue to the greatest extent, and only retain the collagen fibers and elastic fibers in the bovine pericardium slice , to maintain the integrity of the pulmonary artery stent, and then filled with matrix-like gelatin, and finally cross-linked with EDC / NHS to obtain a "chimeric" tissue-engineered bovine pericardium slice.
[0007] Basic steps: After the fresh bovine pericardium slices are fully trimmed, place them in a decellularized solution of 0.25% Triton X-100+0.25% deoxycholate sodium salt+0.02% EDTA, shake at a constant temperature and speed (37°C, 200r / min) 24-hour decellularization treatment; after decellularization, place in sterilized PBS with 1% penicillin and streptomycin + 2ug / ml amphotericin B, shake at constant temperature and speed (37°C, 200r / min)...
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