Chimeric tissue engineering bovine pericardium sheet

Abstract

A chimeric tissue engineered bovine pericardium slice. The middle layer of the bovine pericardium is composed of three parts: cells, matrix, and fibers. The fibers maintain the biomechanical properties of the bovine pericardium, cells exist in the fiber voids, and form connections with the fibers through matrix components. The reasons for calcification, thrombus formation and decay in bovine pericardium that are commonly treated with glutaraldehyde in clinical practice are as follows: 1. Glutaraldehyde fixation can reduce immunogenicity to a certain extent, but there are still heterogeneous cell components remaining, which are cytotoxic , can induce immune rejection, and further cause tissue calcification and decay; 2, the middle space is small, it is difficult for body cells to grow in, and the epitope that induces rejection is exposed; 3, the residual aldehyde group is cytotoxic, and cells are difficult to Survival, low regenerative capacity. In order to effectively solve the above problems, on the basis of decellularization, matrix-like filling and cross-linking were performed to form chimeric tissue-engineered bovine pericardium slices.

Description

technical field [0001] Medicine Tissue Engineering. Background technique [0002] Valve replacement is an effective method for the treatment of severe heart valve diseases. At present, most of the biological valves used in clinical practice are porcine biological valves and bovine pericardial valves treated with glutaraldehyde. Because of their good hemodynamic properties and good tissue compatibility, The short anticoagulant time can reduce thromboembolism or bleeding complications and the inconvenience caused by long-term oral anticoagulant drugs, and is increasingly widely used in clinical practice. However, it is accompanied by problems of aging, decay and calcification, which greatly restrict its long-term benefits. The rise of tissue engineering provides new ideas for the development of cardiovascular patches and artificial heart valves. [0003] The middle layer of normal bovine pericardium is composed of cells, matrix and fibers. Fiber is the main factor to mainta...

AI Technical Summary

Problems solved by technology

After an in-depth study on the bovine pericardial tissue structure, the results confirmed that the bovine pericardial valves commonly used in clinical treatment with glutaraldehyde have the following reasons for rejection, calcification, thrombus formation and unsatisfactory mechanical properties: 1. Although the dialdehyde fixation method can reduce the immunogenicity to a certain extent, there are still heterogeneous c...

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