A targeted complement antibody microcapsule preparation for the treatment of alcoholic liver disease and its preparation method and application
A targeted technology for alcoholic liver disease, applied in biochemical equipment and methods, antibodies, microcapsules, etc., can solve the problems of immune regulation imbalance, low biological activity, and inability to localize, and achieve long-term action time and targeted strong effect
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Embodiment 1
[0030] Effect of complement antibody CR2-Crry on acute alcoholic liver injury (see image 3 ).
[0031] Eighteen C57BL / 6 wild-type mice were used and randomly divided into three groups with 6 mice in each group. In the alcohol treatment + complement antibody protein treatment group, each mouse was given intraperitoneal injection of 0.25mg CR2-Crry 30 minutes before the alcohol gavage; ) by intraperitoneal injection, while the control group was given intragastric administration of isocaloric dextrin solution, and the mice were sacrificed 6 hours later to obtain serum and liver samples. Serum samples were used to detect liver function, and liver tissue samples were tested for C3 deposition by immunofluorescence.
[0032] Test results such as image 3 As shown, the detection results confirmed that the complement antibody can significantly reduce liver damage while inhibiting complement activation.
Embodiment 2
[0033] Example 2: Effect of complement antibody CR2-Crry on chronic alcoholic liver injury.
[0034] Twenty-four C57BL / 6 wild-type mice were used and randomly divided into three groups with 8 mice in each group. The alcoholic fatty liver degeneration model (Alcoholic Fatty Liver, AFL) was fed with liquid alcohol feed (Nantong Trophy Feed Technology Co., Ltd., TP4030D) according to the NIAAA method (National Institute on Alcohol Abuse and Alcoholism), and the control group was fed with isocaloric control feed (Nantong Trofe Feed Technology Co., Ltd., TP4030C), the AFL+CR2-Crry group was given intraperitoneal injection of 0.25 mg CR2-Crry every day after the 10th day of alcohol feed feeding, and the AFL group was given the same amount of NS treatment, and the pups were killed after 6 consecutive days. Serum and liver samples were collected from mice. Serum samples were used to detect liver function and complement levels, and liver tissue samples were used to detect liver fat de...
Embodiment 3
[0037] Effect of complement antibody CR2-Crry on liver injury and liver regeneration after liver resection in the context of alcoholic liver disease.
[0038] The specific modeling process is shown in Example 2. On the basis of chronic alcoholic liver disease, we further adopted a 70% liver resection model, and collected serum and liver tissue samples for related testing 48 hours after the operation.
[0039] Test results such as Figure 5 As shown, the test results confirm that: in the background of chronic alcoholic liver disease, liver resection is performed, and complement antibodies can significantly reduce liver injury and improve liver regeneration after hepatectomy while inhibiting complement activation.
[0040] The application of a targeted complement antibody microcapsule oral liquid preparation is mainly used in the treatment of alcoholic liver disease. The method of use is: take orally, twice a day, 1ml (1.0g antibody) each time, and one month is a course of trea...
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